ASC / Caspase1 (CARD)

Interactions between ASC in detail and Caspase1 in detail (CARD)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
CARD ASC Link Caspase1 GST fusion protein pull-down Human 105-195 In vitro translation Not specified Not specified E.coli 11967258
CARD ASC Link Caspase1 GST fusion protein pull-down Human Full length In vitro translation Not specified Not specified E.coli 11967258
CARD ASC Link Caspase1 GST fusion protein pull-down Not specified Not specified E.coli Not specified Not specified In vitro translation 14634131
CARD ASC Link Caspase1 Yeast two-hybrid Human 105-195 Yeast Human 1-91 Yeast 19337385
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD ASC Link Caspase1 Co-immunoprecipitation HEK293 Human 105-195 Not specified Not specified 11967258
CARD ASC Link Caspase1 Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 11967258
CARD ASC Link Caspase1 Co-immunoprecipitation HEK293 Not specified 92-195 Not specified Not specified 12019269
CARD ASC Link caspase1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 12191486
CARD ASC Link Caspase1 Co-immunoprecipitation PT67 Mouse Full length Mouse Not specified 12667444
CARD ASC Link Caspase1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 14634131
CARD ASC Link Caspase1 Co-immunoprecipitation THP-1 14634131
CARD ASC Link Caspase1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 16585594
CARD ASC Link Caspase1 Co-immunoprecipitation THP-1 16585594
CARD ASC Link Caspase1 Co-immunoprecipitation Rat cortical lysate 19401709
CARD ASC Link Caspase1 Co-immunoprecipitation HEK239 Human Not specified Human Not specified 21862576
CARD ASC Link Caspase1 GST fusion protein pull-down PT67 Mouse Full length Mouse Not specified 12667444
CARD ASC Link Caspase1 Size-exclusion chromatography Mice cerebral cortex 19066616
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
CARD ASC Link Caspase1 Co-immunoprecipitation L122Q(ASC) 14634131
CARD ASC Link Caspase1 Co-immunoprecipitation D27G, R45D (Caspase1) 21862576
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
CARD ASC Link Caspase1 ELISA IL-1 secretion (Inflammasome) 11967258
CARD ASC Link Caspase1 ELISA IL-1b secretion (Inflammasome) 12019269
CARD ASC Link Caspase1 ELISA IL-1b secretion decrease 14634131
CARD ASC Link Caspase1 ELISA IL-1b secretion 16585594
CARD ASC Link Caspase1 Luciferase assay NF-kB activation 21862576
(Link: click this icon to show interactions only between the two corresponding DDs)