Apaf1 / Caspase9 (CARD)

Interactions between Apaf1 in detail and Caspase9 in detail (CARD)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
CARD Apaf1 Link Caspase9 GST fusion protein pull-down Not specified 1-97 E.coli Not specified Not specified In vitro translation 9390557
CARD Apaf1 Link Caspase9 GST fusion protein pull-down Not specified 1-97 E.coli Not specified Not specified In vitro translation 9922454
CARD Apaf1 Link Caspase9 GST fusion protein pull-down Not specified 1-420 In vitro translation Not specified Not specified E.coli 11408476
CARD Apaf1 Link Caspase9 GST fusion protein pull-down Not specified Not specified E.coli Not specified Not specified In vitro translation 11821383
CARD Apaf1 Link Caspase9 GST fusion protein pull-down Human THP-1 lysate Not specified 1-130 E.coli 14993223
CARD Apaf1 Link Caspase9 GST fusion protein pull-down Not specified Not specified E.coli Not specified D32cell lysate 15542838
CARD Apaf1 Link Caspase9 GST fusion protein pull-down Not specified D32cell lysate Not specified Not specified E.coli 15542838
CARD Apaf1 Link Caspase9 In vitro purification protein assembly(Size-exclusion chromatography) Human Full length HeLa Not specified Full length HeLa 10206961
CARD Apaf1 Link Caspase9 In vitro purification protein assembly(Size-exclusion chromatography) Human 1-97 E.coli Human 1-112 E.coli 10376594
CARD Apaf1 Link Caspase9 In vitro purification protein assembly(Size-exclusion chromatography) Not specified THP-1 lysate Not specified THP-1 lysate 11230124
CARD Apaf1 Link Caspase9 In vitro purification protein assembly(Size-exclusion chromatography) Not specified 1-97 E.coli Not specified Full length E.coli 11904389
CARD Apaf1 Link Caspase9 In vitro purification protein assembly(Size-exclusion chromatography) Not specified 1-97 E.coli Not specified 1-132 E.coli 11904389
CARD Apaf1 Link Caspase9 In vitro purification protein assembly(Size-exclusion chromatography) Not specified Not specified E.coli Not specified Not specified E.coli 19494828
CARD Apaf1 Link Caspase9 Yeast two-hybrid Human 1-92 Yeast Human 1-97 Yeast 19337385
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Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD Apaf1 Link Caspase9 Co-immunoprecipitation HeLa S-100 9390557
CARD Apaf1 Link Caspase9 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 9488720
CARD Apaf1 Link Caspase9 Co-immunoprecipitation HEK293 Not specified 1-412 Not specified Full length 9488720
CARD Apaf1 Link Caspase9 Co-immunoprecipitation HEK293 Not specified 1-412 Not specified 1-168 9488720
CARD Apaf1 Link Caspase9 Co-immunoprecipitation HEK293 Not specified Full length Human Not specified 9539746
CARD Apaf1 Link Caspase9 Co-immunoprecipitation HEK293 Not specified 1-559 Human Not specified 9539746
CARD Apaf1 Link Caspase9 Co-immunoprecipitation Rat cardiomyocyte 15253891
CARD Apaf1 Link Caspase9 Size-exclusion chromatography HEK293 10617566
CARD Apaf1 Link Caspase9 Size-exclusion chromatography Human monocytic tumor cell 10692394
CARD Apaf1 Link Caspase9 Size-exclusion chromatography THP-1 11550094
CARD Apaf1 Link Caspase9 Size-exclusion chromatography HeLa 12460989
CARD Apaf1 Link Caspase9 Size-exclusion chromatography Rat cortex 14602082
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Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
CARD Apaf1 Link Caspase9 Structure 1:1 D27A, E40A (Apaf1) R13A, R56A (Caspase9) 3YGS 10376594
CARD Apaf1 Link Caspase9 Gel filtration 1:1 11904389
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Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
CARD Apaf1 Link Caspase9 β-galactosidase staining Apoptosis (Apoptosome) 9390557
CARD Apaf1 Link Caspase9 DAPI staining Apoptosis (Apoptosome) 9488720
CARD Apaf1 Link Caspase9 β-galactosidase staining Apoptosis (Apoptosome) 9539746
CARD Apaf1 Link Caspase9 DAPI staining Apoptosis (Apoptosome) 11408476
CARD Apaf1 Link Caspase9 Caspase cleaved assay Caspase9 activation 10206961
CARD Apaf1 Link Caspase9 Caspase cleaved assay Caspase9 activation (Apoptosome) 11904389
CARD Apaf1 Link Caspase9 Caspase cleaved assay Caspase9 activation 10617566
(Link: click this icon to show interactions only between the two corresponding DDs)