Caspase1 / ASC (CARD)

Interactions between Caspase1 in detail and ASC in detail (CARD)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
CARD Caspase1 Link ASC GST fusion protein pull-down Not specified Not specified E.coli Human 105-195 In vitro translation 11967258
CARD Caspase1 Link ASC GST fusion protein pull-down Not specified Not specified E.coli Human Full length In vitro translation 11967258
CARD Caspase1 Link ASC GST fusion protein pull-down Not specified Not specified In vitro translation Not specified Not specified E.coli 14634131
CARD Caspase1 Link ASC Yeast two-hybrid Human 1-91 Yeast Human 105-195 Yeast 19337385
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD Caspase1 Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Human 105-195 11967258
CARD Caspase1 Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 11967258
CARD Caspase1 Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Not specified 92-195 12019269
CARD caspase1 Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 12191486
CARD Caspase1 Link ASC Co-immunoprecipitation PT67 Mouse Not specified Mouse Full length 12667444
CARD Caspase1 Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 14634131
CARD Caspase1 Link ASC Co-immunoprecipitation THP-1 14634131
CARD Caspase1 Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 16585594
CARD Caspase1 Link ASC Co-immunoprecipitation THP-1 16585594
CARD Caspase1 Link ASC Co-immunoprecipitation Rat cortical lysate 19401709
CARD Caspase1 Link ASC Co-immunoprecipitation HEK239 Human Not specified Human Not specified 21862576
CARD Caspase1 Link ASC GST fusion protein pull-down PT67 Mouse Not specified Mouse Full length 12667444
CARD Caspase1 Link ASC Size-exclusion chromatography Mice cerebral cortex 19066616
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
CARD Caspase1 Link ASC Co-immunoprecipitation L122Q(ASC) 14634131
CARD Caspase1 Link ASC Co-immunoprecipitation D27G, R45D (Caspase1) 21862576
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
CARD Caspase1 Link ASC ELISA IL-1 secretion (Inflammasome) 11967258
CARD Caspase1 Link ASC ELISA IL-1b secretion (Inflammasome) 12019269
CARD Caspase1 Link ASC ELISA IL-1b secretion decrease 14634131
CARD Caspase1 Link ASC ELISA IL-1b secretion 16585594
CARD Caspase1 Link ASC Luciferase assay NF-kB activation 21862576
(Link: click this icon to show interactions only between the two corresponding DDs)