Caspase9 / Apaf1 (CARD)

Interactions between Caspase9 in detail and Apaf1 in detail (CARD)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
CARD Caspase9 Link Apaf1 GST fusion protein pull-down Not specified Not specified In vitro translation Not specified 1-97 E.coli 9390557
CARD Caspase9 Link Apaf1 GST fusion protein pull-down Not specified Not specified In vitro translation Not specified 1-97 E.coli 9922454
CARD Caspase9 Link Apaf1 GST fusion protein pull-down Not specified Not specified E.coli Not specified 1-420 In vitro translation 11408476
CARD Caspase9 Link Apaf1 GST fusion protein pull-down Not specified Not specified In vitro translation Not specified Not specified E.coli 11821383
CARD Caspase9 Link Apaf1 GST fusion protein pull-down Not specified 1-130 E.coli Human THP-1 lysate 14993223
CARD Caspase9 Link Apaf1 GST fusion protein pull-down Not specified D32cell lysate Not specified Not specified E.coli 15542838
CARD Caspase9 Link Apaf1 GST fusion protein pull-down Not specified Not specified E.coli Not specified D32cell lysate 15542838
CARD Caspase9 Link Apaf1 In vitro purification protein assembly(Size-exclusion chromatography) Not specified Full length HeLa Human Full length HeLa 10206961
CARD Caspase9 Link Apaf1 In vitro purification protein assembly(Size-exclusion chromatography) Human 1-112 E.coli Human 1-97 E.coli 10376594
CARD Caspase9 Link Apaf1 In vitro purification protein assembly(Size-exclusion chromatography) Not specified THP-1 lysate Not specified THP-1 lysate 11230124
CARD Caspase9 Link Apaf1 In vitro purification protein assembly(Size-exclusion chromatography) Not specified Full length E.coli Not specified 1-97 E.coli 11904389
CARD Caspase9 Link Apaf1 In vitro purification protein assembly(Size-exclusion chromatography) Not specified 1-132 E.coli Not specified 1-97 E.coli 11904389
CARD Caspase9 Link Apaf1 In vitro purification protein assembly(Size-exclusion chromatography) Not specified Not specified E.coli Not specified Not specified E.coli 19494828
CARD Caspase9 Link Apaf1 Yeast two-hybrid Human 1-97 Yeast Human 1-92 Yeast 19337385
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD Caspase9 Link Apaf1 Co-immunoprecipitation HeLa S-100 9390557
CARD Caspase9 Link Apaf1 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 9488720
CARD Caspase9 Link Apaf1 Co-immunoprecipitation HEK293 Not specified Full length Not specified 1-412 9488720
CARD Caspase9 Link Apaf1 Co-immunoprecipitation HEK293 Not specified 1-168 Not specified 1-412 9488720
CARD Caspase9 Link Apaf1 Co-immunoprecipitation HEK293 Human Not specified Not specified Full length 9539746
CARD Caspase9 Link Apaf1 Co-immunoprecipitation HEK293 Human Not specified Not specified 1-559 9539746
CARD Caspase9 Link Apaf1 Co-immunoprecipitation Rat cardiomyocyte 15253891
CARD Caspase9 Link Apaf1 Size-exclusion chromatography HEK293 10617566
CARD Caspase9 Link Apaf1 Size-exclusion chromatography Human monocytic tumor cell 10692394
CARD Caspase9 Link Apaf1 Size-exclusion chromatography THP-1 11550094
CARD Caspase9 Link Apaf1 Size-exclusion chromatography HeLa 12460989
CARD Caspase9 Link Apaf1 Size-exclusion chromatography Rat cortex 14602082
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
CARD Caspase9 Link Apaf1 Structure 1:1 D27A, E40A (Apaf1) R13A, R56A (Caspase9) 3YGS 10376594
CARD Caspase9 Link Apaf1 Gel filtration 1:1 11904389
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
CARD Caspase9 Link Apaf1 β-galactosidase staining Apoptosis (Apoptosome) 9390557
CARD Caspase9 Link Apaf1 DAPI staining Apoptosis (Apoptosome) 9488720
CARD Caspase9 Link Apaf1 β-galactosidase staining Apoptosis (Apoptosome) 9539746
CARD Caspase9 Link Apaf1 DAPI staining Apoptosis (Apoptosome) 11408476
CARD Caspase9 Link Apaf1 Caspase cleaved assay Caspase9 activation 10206961
CARD Caspase9 Link Apaf1 Caspase cleaved assay Caspase9 activation (Apoptosome) 11904389
CARD Caspase9 Link Apaf1 Caspase cleaved assay Caspase9 activation 10617566
(Link: click this icon to show interactions only between the two corresponding DDs)