FADD / Fas (DD)

Interactions between FADD in detail and Fas in detail (DD)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD FADD Link Fas Affinity purification-mass spectrometry Human 93-184 E.coli Mouse 210-310 E.coli 20935634
DD FADD Link Fas Affinity purification-mass spectrometry Human 93-191 E.coli Human 218-318 E.coli 20935634
DD FADD Link Fas GST fusion protein pull-down Not specified Not specified Not specified Human 175-319 Not specified 7536190
DD FADD Link Fas GST fusion protein pull-down Not specified Not specified Yeast Not specified Not specified Yeast 7538907
DD FADD Link Fas GST fusion protein pull-down Not specified Not specified In vitro translation Not specified Not specified E.coli 8875942
DD FADD Link Fas GST fusion protein pull-down Human 80-205 In vitro translation Murine 165-295 Not specified 9990010
DD FADD Link Fas GST fusion protein pull-down Not specified Not specified In vitro translation Human 174-319 E.coli 10200300
DD FADD Link Fas GST fusion protein pull-down Jurkat lysate Not specified 191-335 E.coli 12721308
DD FADD Link Fas GST fusion protein pull-down Human Full length In vitro translation Human Not specified Not specified 14573612
DD FADD Link Fas His fusion protein pull-down Human 93-208 E.coli Human 223-335 E.coli 19118384
DD FADD Link Fas His fusion protein pull-down Not specified Not specified E.coli Not specified Not specified E.coli 20935634
DD FADD Link Fas In vitro purification protein assembly(Pull down) Not specified Not specified HEK293 Not specified 1-304 E.coli 7538907
DD FADD Link Fas In vitro purification protein assembly(Size-exclusion chromatography) Human 93-208 E.coli Human 223-335 E.coli 19118384
DD FADD Link Fas In vitro purification protein assembly(Size-exclusion chromatography) Human 93-184 E.coli Human 210-310 E.coli 20935634
DD FADD Link Fas In vitro purification protein assembly(Surface plasmon resonance) Not specified 89-183 E.coli Human Not specified E.coli 10347191
DD FADD Link Fas Yeast two-hybrid Not specified 130-245 Yeast Human 175-319 Yeast 7536190
DD FADD Link Fas Yeast two-hybrid Not specified 130-245 Yeast Human 200-319 Yeast 7536190
DD FADD Link Fas Yeast two-hybrid Not specified Not specified In vitro translation Not specified 1-304 E.coli 7538907
DD FADD Link Fas Yeast two-hybrid Not specified 92-208 Yeast Not specified 210-319 Yeast 8642271
DD FADD Link Fas Yeast two-hybrid Human 80-208 Yeast Human 177-335 Yeast 11464214
DD FADD Link Fas Yeast two-hybrid Not specified Full length Yeast Human 191-313 Yeast 12107169
DD FADD Link Fas Yeast two-hybrid Not specified Not specified Yeast Not specified 177-335 Yeast 15173180
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD FADD Link Fas Jurkat 21557211
DD FADD Link Fas Co-Immunofluorescence staining Jurkat 12702723
DD FADD Link Fas Co-Immunofluorescence staining BJAB Not specified Not specified Not specified Not specified 16498403
DD FADD Link Fas Co-immunoprecipitation HeLa Not specified Not specified Not specified Not specified 7536190
DD FADD Link Fas Co-immunoprecipitation HEK293 Not specified Not specified Not specified 1-304 7538907
DD FADD Link Fas Co-immunoprecipitation HEK293 Not specified 80-208 Not specified Not specified 8617770
DD FADD Link Fas Co-immunoprecipitation HEK293 Not specified 2-208 Not specified Not specified 8617770
DD FADD Link Fas Co-immunoprecipitation A20 Murine B cell 8649383
DD FADD Link Fas Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 9082980
DD FADD Link Fas Co-immunoprecipitation BJAB 9325306
DD FADD Link Fas Co-immunoprecipitation HEK293 Human Not specified Human 19-335 9430227
DD FADD Link Fas Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 9714541
DD FADD Link Fas Co-immunoprecipitation DT-40 chicken lymphoma B cell 9880544
DD FADD Link Fas Co-immunoprecipitation HE29 10075697
DD FADD Link Fas Co-immunoprecipitation HEK293 Not used Human 174-319 10200300
DD FADD Link Fas Co-immunoprecipitation A20 Murine B cell 10452970
DD FADD Link Fas Co-immunoprecipitation H9 10607716
DD FADD Link Fas Co-immunoprecipitation SHEP neuroblastoma cell 10919673
DD FADD Link Fas Co-immunoprecipitation CD40L-induced B blast 10975811
DD FADD Link Fas Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 11032752
DD FADD Link Fas Co-immunoprecipitation Tonsillar germinal center B cell 11181697
DD FADD Link Fas Co-immunoprecipitation HT29 11313933
DD FADD Link Fas Co-immunoprecipitation Jurkat 11729181
DD FADD Link Fas Co-immunoprecipitation SKW6.4 11729181
DD FADD Link Fas Co-immunoprecipitation SKW6.4 11739735
DD FADD Link Fas Co-immunoprecipitation H9 11739735
DD FADD Link Fas Co-immunoprecipitation Mouse brain cell 11978827
DD FADD Link Fas Co-immunoprecipitation SKW6.4 12110583
DD FADD Link Fas Co-immunoprecipitation H9 12110583
DD FADD Link Fas Co-immunoprecipitation Human Jurkat cell 12878044
DD FADD Link Fas Co-immunoprecipitation HuT-78 12878044
DD FADD Link Fas Co-immunoprecipitation MCF-7 Human Full length Not used 14573612
DD FADD Link Fas Co-immunoprecipitation HEK293 15383280
DD FADD Link Fas Co-immunoprecipitation Human neuronal progenitor cell 15545353
DD FADD Link Fas Co-immunoprecipitation SKW6.4 16498403
DD FADD Link Fas Co-immunoprecipitation SKW6.4 17159907
DD FADD Link Fas Co-immunoprecipitation T98G 18328427
DD FADD Link Fas Co-immunoprecipitation LN-18 18328427
DD FADD Link Fas Fluorescence resonance energy transfer HEK293 Human Not specified Human Full length 20935634
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DD FADD Link Fas Mass spectrometry, MALS 5:5, 5:6, 5:7 20935634
DD FADD Link Fas Co-immunoprecipitation V238N, D244V, Q257X, A241D, I294S (Fas) 10200300
DD FADD Link Fas Co-immunoprecipitation R113E, D123R, R166E, Q169A, D175R, D106A, K125A, R142A (FADD) 14573612
DD FADD Link Fas Fluorescence resonance energy transfer D260V, D260Y, A257D, E261K, T270K, E272K, Q283K (Fas) R117E, D123R, R135E, R172E (FADD) 20935634
DD FADD Link Fas GST fusion protein pull-down T225P, Q257X, A241D, I294S, D244V, R234Q, L278X, R234P, Q260X, I238N (Fas) 10200300
DD FADD Link Fas GST fusion protein pull-down R113E, R117E, D123R, R166E, L172E, D175R, D106A, K125A, R142A, R146A (FADD) 14573612
DD FADD Link Fas Structure 4:4 L172A (FADD) 3EZQ 19118384
DD FADD Link Fas His fusion protein pull-down E261K, E272K, T270K, R250E, Q283K, K287D, R250Q, D260Y, A257D, E272K, T270K (fas) 20935634
DD FADD Link Fas Structure 5:5 3OQ9 20935634
DD FADD Link Fas Surface plasmon resonance 1.0E+07M-1 R110A, R113A, R117A, E118A, V121N, E123A (FADD) 10347191
DD FADD Link Fas Yeast two-hybrid R117A (FADD) 12107169
DD FADD Link Fas Yeast two-hybrid V108E(FADD) 15173180
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DD FADD Link Fas MTT assay Apoptosis (DISC) 21557211
DD FADD Link Fas MTT assay Apoptosis (DISC) 8617770
DD FADD Link Fas Propidium iodide staining Apoptosis (DISC) 8649383
DD FADD Link Fas Propidium iodide staining Apoptosis (DISC) 9325306
DD FADD Link Fas Propidium iodide staining Apoptosis (DISC) 10607716
DD FADD Link Fas Propidium iodide staining Apoptosis (DISC) 10919673
(Link: click this icon to show interactions only between the two corresponding DDs)