Fas / FADD (DD)

Interactions between Fas in detail and FADD in detail (DD)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD Fas Link FADD Affinity purification-mass spectrometry Mouse 210-310 E.coli Human 93-184 E.coli 20935634
DD Fas Link FADD Affinity purification-mass spectrometry Human 218-318 E.coli Human 93-191 E.coli 20935634
DD Fas Link FADD GST fusion protein pull-down Human 175-319 Not specified Not specified Not specified Not specified 7536190
DD Fas Link FADD GST fusion protein pull-down Not specified Not specified Yeast Not specified Not specified Yeast 7538907
DD Fas Link FADD GST fusion protein pull-down Not specified Not specified E.coli Not specified Not specified In vitro translation 8875942
DD Fas Link FADD GST fusion protein pull-down Murine 165-295 Not specified Human 80-205 In vitro translation 9990010
DD Fas Link FADD GST fusion protein pull-down Human 174-319 E.coli Not specified Not specified In vitro translation 10200300
DD Fas Link FADD GST fusion protein pull-down Not specified 191-335 E.coli Jurkat lysate 12721308
DD Fas Link FADD GST fusion protein pull-down Human Not specified Not specified Human Full length In vitro translation 14573612
DD Fas Link FADD His fusion protein pull-down Human 223-335 E.coli Human 93-208 E.coli 19118384
DD Fas Link FADD His fusion protein pull-down Not specified Not specified E.coli Not specified Not specified E.coli 20935634
DD Fas Link FADD In vitro purification protein assembly(Pull down) Not specified 1-304 E.coli Not specified Not specified HEK293 7538907
DD Fas Link FADD In vitro purification protein assembly(Size-exclusion chromatography) Human 223-335 E.coli Human 93-208 E.coli 19118384
DD Fas Link FADD In vitro purification protein assembly(Size-exclusion chromatography) Human 210-310 E.coli Human 93-184 E.coli 20935634
DD Fas Link FADD In vitro purification protein assembly(Surface plasmon resonance) Human Not specified E.coli Not specified 89-183 E.coli 10347191
DD Fas Link FADD Yeast two-hybrid Human 175-319 Yeast Not specified 130-245 Yeast 7536190
DD Fas Link FADD Yeast two-hybrid Human 200-319 Yeast Not specified 130-245 Yeast 7536190
DD Fas Link FADD Yeast two-hybrid Not specified 1-304 E.coli Not specified Not specified In vitro translation 7538907
DD Fas Link FADD Yeast two-hybrid Not specified 210-319 Yeast Not specified 92-208 Yeast 8642271
DD Fas Link FADD Yeast two-hybrid Human 177-335 Yeast Human 80-208 Yeast 11464214
DD Fas Link FADD Yeast two-hybrid Human 191-313 Yeast Not specified Full length Yeast 12107169
DD Fas Link FADD Yeast two-hybrid Not specified 177-335 Yeast Not specified Not specified Yeast 15173180
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD Fas Link FADD Jurkat 21557211
DD Fas Link FADD Co-Immunofluorescence staining Jurkat 12702723
DD Fas Link FADD Co-Immunofluorescence staining BJAB Not specified Not specified Not specified Not specified 16498403
DD Fas Link FADD Co-immunoprecipitation HeLa Not specified Not specified Not specified Not specified 7536190
DD Fas Link FADD Co-immunoprecipitation HEK293 Not specified 1-304 Not specified Not specified 7538907
DD Fas Link FADD Co-immunoprecipitation HEK293 Not specified Not specified Not specified 80-208 8617770
DD Fas Link FADD Co-immunoprecipitation HEK293 Not specified Not specified Not specified 2-208 8617770
DD Fas Link FADD Co-immunoprecipitation A20 Murine B cell 8649383
DD Fas Link FADD Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 9082980
DD Fas Link FADD Co-immunoprecipitation BJAB 9325306
DD Fas Link FADD Co-immunoprecipitation HEK293 Human 19-335 Human Not specified 9430227
DD Fas Link FADD Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 9714541
DD Fas Link FADD Co-immunoprecipitation DT-40 chicken lymphoma B cell 9880544
DD Fas Link FADD Co-immunoprecipitation HE29 10075697
DD Fas Link FADD Co-immunoprecipitation HEK293 Human 174-319 Not used 10200300
DD Fas Link FADD Co-immunoprecipitation A20 Murine B cell 10452970
DD Fas Link FADD Co-immunoprecipitation H9 10607716
DD Fas Link FADD Co-immunoprecipitation SHEP neuroblastoma cell 10919673
DD Fas Link FADD Co-immunoprecipitation CD40L-induced B blast 10975811
DD Fas Link FADD Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 11032752
DD Fas Link FADD Co-immunoprecipitation Tonsillar germinal center B cell 11181697
DD Fas Link FADD Co-immunoprecipitation HT29 11313933
DD Fas Link FADD Co-immunoprecipitation Jurkat 11729181
DD Fas Link FADD Co-immunoprecipitation SKW6.4 11729181
DD Fas Link FADD Co-immunoprecipitation SKW6.4 11739735
DD Fas Link FADD Co-immunoprecipitation H9 11739735
DD Fas Link FADD Co-immunoprecipitation Mouse brain cell 11978827
DD Fas Link FADD Co-immunoprecipitation SKW6.4 12110583
DD Fas Link FADD Co-immunoprecipitation H9 12110583
DD Fas Link FADD Co-immunoprecipitation Human Jurkat cell 12878044
DD Fas Link FADD Co-immunoprecipitation HuT-78 12878044
DD Fas Link FADD Co-immunoprecipitation MCF-7 Not used Human Full length 14573612
DD Fas Link FADD Co-immunoprecipitation HEK293 15383280
DD Fas Link FADD Co-immunoprecipitation Human neuronal progenitor cell 15545353
DD Fas Link FADD Co-immunoprecipitation SKW6.4 16498403
DD Fas Link FADD Co-immunoprecipitation SKW6.4 17159907
DD Fas Link FADD Co-immunoprecipitation T98G 18328427
DD Fas Link FADD Co-immunoprecipitation LN-18 18328427
DD Fas Link FADD Fluorescence resonance energy transfer HEK293 Human Full length Human Not specified 20935634
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DD Fas Link FADD Mass spectrometry, MALS 5:5, 5:6, 5:7 20935634
DD Fas Link FADD Co-immunoprecipitation V238N, D244V, Q257X, A241D, I294S (Fas) 10200300
DD Fas Link FADD Co-immunoprecipitation R113E, D123R, R166E, Q169A, D175R, D106A, K125A, R142A (FADD) 14573612
DD Fas Link FADD Fluorescence resonance energy transfer D260V, D260Y, A257D, E261K, T270K, E272K, Q283K (Fas) R117E, D123R, R135E, R172E (FADD) 20935634
DD Fas Link FADD GST fusion protein pull-down T225P, Q257X, A241D, I294S, D244V, R234Q, L278X, R234P, Q260X, I238N (Fas) 10200300
DD Fas Link FADD GST fusion protein pull-down R113E, R117E, D123R, R166E, L172E, D175R, D106A, K125A, R142A, R146A (FADD) 14573612
DD Fas Link FADD Structure 4:4 L172A (FADD) 3EZQ 19118384
DD Fas Link FADD His fusion protein pull-down E261K, E272K, T270K, R250E, Q283K, K287D, R250Q, D260Y, A257D, E272K, T270K (fas) 20935634
DD Fas Link FADD Structure 5:5 3OQ9 20935634
DD Fas Link FADD Surface plasmon resonance 1.0E+07M-1 R110A, R113A, R117A, E118A, V121N, E123A (FADD) 10347191
DD Fas Link FADD Yeast two-hybrid R117A (FADD) 12107169
DD Fas Link FADD Yeast two-hybrid V108E(FADD) 15173180
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DD Fas Link FADD MTT assay Apoptosis (DISC) 21557211
DD Fas Link FADD MTT assay Apoptosis (DISC) 8617770
DD Fas Link FADD Propidium iodide staining Apoptosis (DISC) 8649383
DD Fas Link FADD Propidium iodide staining Apoptosis (DISC) 9325306
DD Fas Link FADD Propidium iodide staining Apoptosis (DISC) 10607716
DD Fas Link FADD Propidium iodide staining Apoptosis (DISC) 10919673
(Link: click this icon to show interactions only between the two corresponding DDs)