IRAK4 / Myd88 (DD)

Interactions between IRAK4 in detail and Myd88 in detail (DD)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD IRAK4 Link Myd88 His fusion protein pull-down Human 1-185 E.coli Human 1-157 E.coli 19167362
DD IRAK4 Link Myd88 In vitro purification protein assembly(chemical crosslinking) Human 1-152 E.coli Human 1-110 E.coli 19592493
DD IRAK4 Link Myd88 In vitro purification protein assembly(Size-exclusion chromatography) Human 1-152 E.coli Human 1-110 E.coli 19592493
DD IRAK4 Link Myd88 In vitro purification protein assembly(Size-exclusion chromatography) Human 1-117 E.coli Human 1-157 E.coli 20966070
DD IRAK4 Link Myd88 Yeast two-hybrid Not specified Full length Yeast Not specified Full length Yeast 12538665
DD IRAK4 Link Myd88 Yeast two-hybrid Not specified Full length Yeast Not specified 1-172 Yeast 12538665
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD IRAK4 Link Myd88 Co-immunoprecipitation HEK293 Human 1-215 Human Full length 11960013
DD IRAK4 Link Myd88 Co-immunoprecipitation HEK293 Human Not specified Not specified Not specified 15905496
DD IRAK4 Link Myd88 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 19679662
DD IRAK4 Link Myd88 Co-immunoprecipitation HEK293 Human 1-117 Human 1-157 20966070
DD IRAK4 Link Myd88 Co-immunoprecipitation GCB DLBCL line BAJB Not used Not specified Not specified 21179087
DD IRAK4 Link Myd88 His fusion protein pull-down E.coli Human 4-106 Human 20-117 20485341
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DD IRAK4 Link Myd88 Co-immunoprecipitation E52A and E53A, Y58A, K95A (Myd88) 19679662
DD IRAK4 Link Myd88 Co-immunoprecipitation S34Y, R98C (Myd88) 20966070
DD IRAK4 Link Myd88 Structure 6:4:4 N16E and R20E, E69K, T76E, N78E, R12A (IRAK4) V43D, E52K, I61A, Y58A and R62A, R62E, A44E (Myd88) 3MOP 20485341
DD IRAK4 Link Myd88 Gel filtration 4:7, 4:8 19592493
DD IRAK4 Link Myd88 Gel filtration 7:4, 8:4 19592493
DD IRAK4 Link Myd88 In vitro purification protein assembly(Size-exclusion chromatography) S34Y, R98C (Myd88) 20966070
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DD IRAK4 Link Myd88 Luciferase assay NF-kB activation (Myddosome) 20966070
DD IRAK4 Link Myd88 Kinase assay IRAK4 mediated IRAK1 phosphorylation 12538665
(Link: click this icon to show interactions only between the two corresponding DDs)