Myd88 / IRAK4 (DD)

Interactions between Myd88 in detail and IRAK4 in detail (DD)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD Myd88 Link IRAK4 His fusion protein pull-down Human 1-157 E.coli Human 1-185 E.coli 19167362
DD Myd88 Link IRAK4 In vitro purification protein assembly(chemical crosslinking) Human 1-110 E.coli Human 1-152 E.coli 19592493
DD Myd88 Link IRAK4 In vitro purification protein assembly(Size-exclusion chromatography) Human 1-110 E.coli Human 1-152 E.coli 19592493
DD Myd88 Link IRAK4 In vitro purification protein assembly(Size-exclusion chromatography) Human 1-157 E.coli Human 1-117 E.coli 20966070
DD Myd88 Link IRAK4 Yeast two-hybrid Not specified Full length Yeast Not specified Full length Yeast 12538665
DD Myd88 Link IRAK4 Yeast two-hybrid Not specified 1-172 Yeast Not specified Full length Yeast 12538665
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD Myd88 Link IRAK4 Co-immunoprecipitation HEK293 Human Full length Human 1-215 11960013
DD Myd88 Link IRAK4 Co-immunoprecipitation HEK293 Not specified Not specified Human Not specified 15905496
DD Myd88 Link IRAK4 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 19679662
DD Myd88 Link IRAK4 Co-immunoprecipitation HEK293 Human 1-157 Human 1-117 20966070
DD Myd88 Link IRAK4 Co-immunoprecipitation GCB DLBCL line BAJB Not specified Not specified Not used 21179087
DD Myd88 Link IRAK4 His fusion protein pull-down E.coli Human 20-117 Human 4-106 20485341
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DD Myd88 Link IRAK4 Co-immunoprecipitation E52A and E53A, Y58A, K95A (Myd88) 19679662
DD Myd88 Link IRAK4 Co-immunoprecipitation S34Y, R98C (Myd88) 20966070
DD Myd88 Link IRAK4 Structure 6:4:4 N16E and R20E, E69K, T76E, N78E, R12A (IRAK4) V43D, E52K, I61A, Y58A and R62A, R62E, A44E (Myd88) 3MOP 20485341
DD Myd88 Link IRAK4 Gel filtration 4:7, 4:8 19592493
DD Myd88 Link IRAK4 Gel filtration 7:4, 8:4 19592493
DD Myd88 Link IRAK4 In vitro purification protein assembly(Size-exclusion chromatography) S34Y, R98C (Myd88) 20966070
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DD Myd88 Link IRAK4 Luciferase assay NF-kB activation (Myddosome) 20966070
DD Myd88 Link IRAK4 Kinase assay IRAK4 mediated IRAK1 phosphorylation 12538665
(Link: click this icon to show interactions only between the two corresponding DDs)