TNFR1 / TRADD (DD)

Interactions between TNFR1 in detail and TRADD in detail (DD)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD TNFR1 Link TRADD GST fusion protein pull-down Human Not specified Not specified Human Full length In vitro translation 7758105
DD TNFR1 Link TRADD GST fusion protein pull-down Human Not specified Not specified Human 1-305 In vitro translation 7758105
DD TNFR1 Link TRADD GST fusion protein pull-down Human Not specified Not specified Human 195-312 In vitro translation 7758105
DD TNFR1 Link TRADD GST fusion protein pull-down Human 214-426 Not specified Human 196-312 In vitro translation 8621670
DD TNFR1 Link TRADD GST fusion protein pull-down Not specified Not specified E.coli Not specified Not specified In vitro translation 8875942
DD TNFR1 Link TRADD GST fusion protein pull-down Not specified 326-426 E.coli Not specified Not specified E.coli 11231290
DD TNFR1 Link TRADD GST fusion protein pull-down Not specified 237-455 E.coli Jurkat lysate 12721308
DD TNFR1 Link TRADD GST fusion protein pull-down Not specified 195-312 E.coli Not specified 243-455 In vitro translation 16006552
DD TNFR1 Link TRADD Yeast two-hybrid Human 214-426 Yeast Human Full length Yeast 7758105
DD TNFR1 Link TRADD Yeast two-hybrid Not specified 326-426 Yeast Not specified 195-312 Yeast 8642271
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD TNFR1 Link TRADD Rat primary hepatocyte 21741934
DD TNFR1 Link TRADD Co-immunoprecipitation HEK293 Human Not specified Human Not specified 7758105
DD TNFR1 Link TRADD Co-immunoprecipitation U937 8565075
DD TNFR1 Link TRADD Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 8612133
DD TNFR1 Link TRADD Co-immunoprecipitation HEK293 Not specified Not specified Human 112-312 8621670
DD TNFR1 Link TRADD Co-immunoprecipitation U937 8943045
DD TNFR1 Link TRADD Co-immunoprecipitation HEK293 Not specified 37-205 Not specified Not specified 9082980
DD TNFR1 Link TRADD Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 9915703
DD TNFR1 Link TRADD Co-immunoprecipitation U937 9916731
DD TNFR1 Link TRADD Co-immunoprecipitation HEK293 Not used Not specified Not specified 10187805
DD TNFR1 Link TRADD Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 10358032
DD TNFR1 Link TRADD Co-immunoprecipitation L929 10463949
DD TNFR1 Link TRADD Co-immunoprecipitation Fibroblast 10795740
DD TNFR1 Link TRADD Co-immunoprecipitation Jurkat Not specified Not specified Not specified Not specified 11002422
DD TNFR1 Link TRADD Co-immunoprecipitation Jurkat 12167698
DD TNFR1 Link TRADD Co-immunoprecipitation Rat brain hippocampus 12786973
DD TNFR1 Link TRADD Co-immunoprecipitation Jurkat 16611992
DD TNFR1 Link TRADD Co-immunoprecipitation L929 16798936
DD TNFR1 Link TRADD Co-immunoprecipitation Human TLE hammacampus lysate 16919273
DD TNFR1 Link TRADD Co-immunoprecipitation HT1080 18939944
DD TNFR1 Link TRADD Co-immunoprecipitation Mouse seizure hippocampal 21760970
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DD TNFR1 Link TRADD GST fusion protein pull-down 196-312 substituted A(TRADD) 8621670
DD TNFR1 Link TRADD GST fusion protein pull-down Q220E, R224E, D246R, F266E, Q267A, A275D (TRADD) R341E, R437A, G403E, E406R (TNFR1) 16006552
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DD TNFR1 Link TRADD IKK assay NF-kB activation (DISC) 10795740
DD TNFR1 Link TRADD Propidium iodide staining Apoptosis (DISC) NF-kB activation (DISC) 16611992
(Link: click this icon to show interactions only between the two corresponding DDs)