TRADD / TNFR1 (DD)

Interactions between TRADD in detail and TNFR1 in detail (DD)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD TRADD Link TNFR1 GST fusion protein pull-down Human Full length In vitro translation Human Not specified Not specified 7758105
DD TRADD Link TNFR1 GST fusion protein pull-down Human 1-305 In vitro translation Human Not specified Not specified 7758105
DD TRADD Link TNFR1 GST fusion protein pull-down Human 195-312 In vitro translation Human Not specified Not specified 7758105
DD TRADD Link TNFR1 GST fusion protein pull-down Human 196-312 In vitro translation Human 214-426 Not specified 8621670
DD TRADD Link TNFR1 GST fusion protein pull-down Not specified Not specified In vitro translation Not specified Not specified E.coli 8875942
DD TRADD Link TNFR1 GST fusion protein pull-down Not specified Not specified E.coli Not specified 326-426 E.coli 11231290
DD TRADD Link TNFR1 GST fusion protein pull-down Jurkat lysate Not specified 237-455 E.coli 12721308
DD TRADD Link TNFR1 GST fusion protein pull-down Not specified 243-455 In vitro translation Not specified 195-312 E.coli 16006552
DD TRADD Link TNFR1 Yeast two-hybrid Human Full length Yeast Human 214-426 Yeast 7758105
DD TRADD Link TNFR1 Yeast two-hybrid Not specified 195-312 Yeast Not specified 326-426 Yeast 8642271
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD TRADD Link TNFR1 Rat primary hepatocyte 21741934
DD TRADD Link TNFR1 Co-immunoprecipitation HEK293 Human Not specified Human Not specified 7758105
DD TRADD Link TNFR1 Co-immunoprecipitation U937 8565075
DD TRADD Link TNFR1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 8612133
DD TRADD Link TNFR1 Co-immunoprecipitation HEK293 Human 112-312 Not specified Not specified 8621670
DD TRADD Link TNFR1 Co-immunoprecipitation U937 8943045
DD TRADD Link TNFR1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified 37-205 9082980
DD TRADD Link TNFR1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 9915703
DD TRADD Link TNFR1 Co-immunoprecipitation U937 9916731
DD TRADD Link TNFR1 Co-immunoprecipitation HEK293 Not specified Not specified Not used 10187805
DD TRADD Link TNFR1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 10358032
DD TRADD Link TNFR1 Co-immunoprecipitation L929 10463949
DD TRADD Link TNFR1 Co-immunoprecipitation Fibroblast 10795740
DD TRADD Link TNFR1 Co-immunoprecipitation Jurkat Not specified Not specified Not specified Not specified 11002422
DD TRADD Link TNFR1 Co-immunoprecipitation Jurkat 12167698
DD TRADD Link TNFR1 Co-immunoprecipitation Rat brain hippocampus 12786973
DD TRADD Link TNFR1 Co-immunoprecipitation Jurkat 16611992
DD TRADD Link TNFR1 Co-immunoprecipitation L929 16798936
DD TRADD Link TNFR1 Co-immunoprecipitation Human TLE hammacampus lysate 16919273
DD TRADD Link TNFR1 Co-immunoprecipitation HT1080 18939944
DD TRADD Link TNFR1 Co-immunoprecipitation Mouse seizure hippocampal 21760970
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DD TRADD Link TNFR1 GST fusion protein pull-down 196-312 substituted A(TRADD) 8621670
DD TRADD Link TNFR1 GST fusion protein pull-down Q220E, R224E, D246R, F266E, Q267A, A275D (TRADD) R341E, R437A, G403E, E406R (TNFR1) 16006552
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DD TRADD Link TNFR1 IKK assay NF-kB activation (DISC) 10795740
DD TRADD Link TNFR1 Propidium iodide staining Apoptosis (DISC) NF-kB activation (DISC) 16611992
(Link: click this icon to show interactions only between the two corresponding DDs)