Caspase8 / FADD (DED)

Interactions between Caspase8 in detail and FADD in detail (DED)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DED Caspase8 Link FADD Fluorescence resonance energy transfer ` 11717445
DED Caspase8 Link FADD GST fusion protein pull-down HuH-7 lysate Not specified Full length E.coli 12407100
DED Caspase8 Link FADD His fusion protein pull-down Not specified 1-479 In vitro translation Not specified Full length Not specified 8681377
DED Caspase8 Link FADD His fusion protein pull-down Human Not specified In vitro translation Not specified Not specified Not specified 9045686
DED Caspase8 Link FADD His fusion protein pull-down Human 1-479 In vitro translation Not specified Not specified In vitro translation 12010809
DED Caspase8 Link FADD In vitro purification protein assembly(2D gel electrophoresis) Not specified 1-479 In vitro translation Not specified Full length HEK293 8681377
DED Caspase8 Link FADD Yeast two-hybrid Human Not specified Yeast Human Full length Yeast 8681376
DED Caspase8 Link FADD Yeast two-hybrid Human Not specified Yeast Human 1-79 Yeast 8681376
DED Caspase8 Link FADD Yeast two-hybrid Mouse 1-202 Yeast Mouse 1-96 Yeast 10526240
DED Caspase8 Link FADD Yeast two-hybrid Human Full length Yeast Not specified Not specified Yeast 15782135
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DED Caspase8 Link FADD HSC3 21525013
DED Caspase8 Link FADD Co-immunoprecipitation HEK293 Human Not specified Human Not specified 8681376
DED Caspase8 Link FADD Co-immunoprecipitation T47D Not specified Not specified Human Not specified 9582077
DED Caspase8 Link FADD Co-immunoprecipitation Jurkat A3 11432804
DED Caspase8 Link FADD Co-immunoprecipitation MCF-7 Human 1-276 Not used 12010809
DED Caspase8 Link FADD Co-immunoprecipitation HEK293 Human 2-177 Human 2-208 12220669
DED Caspase8 Link FADD Co-immunoprecipitation HT1080 12887920
DED Caspase8 Link FADD Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 15701651
DED Caspase8 Link FADD Co-immunoprecipitation SKW6.4 17159907
DED Caspase8 Link FADD Co-immunoprecipitation H2009 18485876
DED Caspase8 Link FADD Co-immunoprecipitation Panc-1 18485876
DED Caspase8 Link FADD Co-immunoprecipitation HT-1080 19060883
DED Caspase8 Link FADD Co-immunoprecipitation HT-29 19524512
DED Caspase8 Link FADD Co-immunoprecipitation H2009 20547836
DED Caspase8 Link FADD Co-immunoprecipitation SH-EP 22890322
DED Caspase8 Link FADD Co-immunoprecipitation LAN-5 22890322
DED Caspase8 Link FADD Fluorescence resonance energy transfer HEK293 Not specified Full length Not specified Not specified 11717445
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DED Caspase8 Link FADD Co-immunoprecipitation F25G, F35V (FADD) 9582077
DED Caspase8 Link FADD Co-immunoprecipitation K33Q and K35Q, F25G (FADD) 12220669
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DED Caspase8 Link FADD β-galactosidase staining Apoptosis (DISC) 8681376
DED Caspase8 Link FADD DNA fragmentation assay Apoptosis (DISC) 11432804
DED Caspase8 Link FADD Propidium iodide staining Apoptosis (DISC) 12010809
DED Caspase8 Link FADD Hoechst staining Apoptosis (DISC) 12887920
DED Caspase8 Link FADD PARP cleaved assay Apoptosis (DISC) 18485876
DED Caspase8 Link FADD MTT assay Apoptosis (DISC) 22890322
DED Caspase8 Link FADD DAPI staining Apoptosis (DISC) 9045686
(Link: click this icon to show interactions only between the two corresponding DDs)