Caspase8 / FADD (DED)
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In vitro interaction | |||||||||||||
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DD1 | DD2 | Reference | |||||||||||
Family | DD1 | DD2 | Method | Species | Region | Expression | Species | Region | Expression | ||||
DED | Caspase8 |
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FADD | Fluorescence resonance energy transfer | ` | 11717445 | |||||||
DED | Caspase8 |
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FADD | GST fusion protein pull-down | HuH-7 lysate | Not specified | Full length | E.coli | 12407100 | ||||
DED | Caspase8 |
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FADD | His fusion protein pull-down | Not specified | 1-479 | In vitro translation | Not specified | Full length | Not specified | 8681377 | ||
DED | Caspase8 |
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FADD | His fusion protein pull-down | Human | Not specified | In vitro translation | Not specified | Not specified | Not specified | 9045686 | ||
DED | Caspase8 |
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FADD | His fusion protein pull-down | Human | 1-479 | In vitro translation | Not specified | Not specified | In vitro translation | 12010809 | ||
DED | Caspase8 |
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FADD | In vitro purification protein assembly(2D gel electrophoresis) | Not specified | 1-479 | In vitro translation | Not specified | Full length | HEK293 | 8681377 | ||
DED | Caspase8 |
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FADD | Yeast two-hybrid | Human | Not specified | Yeast | Human | Full length | Yeast | 8681376 | ||
DED | Caspase8 |
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FADD | Yeast two-hybrid | Human | Not specified | Yeast | Human | 1-79 | Yeast | 8681376 | ||
DED | Caspase8 |
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FADD | Yeast two-hybrid | Mouse | 1-202 | Yeast | Mouse | 1-96 | Yeast | 10526240 | ||
DED | Caspase8 |
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FADD | Yeast two-hybrid | Human | Full length | Yeast | Not specified | Not specified | Yeast | 15782135 |
(
: click this icon to show interactions only between the two corresponding DDs)

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In vivo interaction | |||||||||||||
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Endogenous expression |
Overexpression | DD1 | DD2 | Reference | |||||||||
Family | DD1 | DD2 | Method | Species | Region | Species | Region | ||||||
DED | Caspase8 |
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FADD | HSC3 | 21525013 | ||||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | HEK293 | Human | Not specified | Human | Not specified | 8681376 | |||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | T47D | Not specified | Not specified | Human | Not specified | 9582077 | |||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | Jurkat A3 | 11432804 | |||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | MCF-7 | Human | 1-276 | Not used | 12010809 | ||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | HEK293 | Human | 2-177 | Human | 2-208 | 12220669 | |||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | HT1080 | 12887920 | |||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | HEK293 | Not specified | Not specified | Human | Full length | 15701651 | |||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | SKW6.4 | 17159907 | |||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | H2009 | 18485876 | |||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | Panc-1 | 18485876 | |||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | HT-1080 | 19060883 | |||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | HT-29 | 19524512 | |||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | H2009 | 20547836 | |||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | SH-EP | 22890322 | |||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | LAN-5 | 22890322 | |||||||
DED | Caspase8 |
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FADD | Fluorescence resonance energy transfer | HEK293 | Not specified | Full length | Not specified | Not specified | 11717445 |
(
: click this icon to show interactions only between the two corresponding DDs)

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Characterization | |||||||||||
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Stoichiometry | Affinity | Protein-Protein interface | Reference | ||||||||
Family | DD1 | DD2 | Method | Mutation | Complex structure | ||||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | F25G, F35V (FADD) | 9582077 | |||||
DED | Caspase8 |
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FADD | Co-immunoprecipitation | K33Q and K35Q, F25G (FADD) | 12220669 |
(
: click this icon to show interactions only between the two corresponding DDs)

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Functional Role | |||||||||||
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Family | DD1 | DD2 | Method | Death-related | Death-unrelated | Reference | |||||
DED | Caspase8 |
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FADD | β-galactosidase staining | Apoptosis (DISC) | 8681376 | |||||
DED | Caspase8 |
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FADD | DNA fragmentation assay | Apoptosis (DISC) | 11432804 | |||||
DED | Caspase8 |
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FADD | Propidium iodide staining | Apoptosis (DISC) | 12010809 | |||||
DED | Caspase8 |
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FADD | Hoechst staining | Apoptosis (DISC) | 12887920 | |||||
DED | Caspase8 |
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FADD | PARP cleaved assay | Apoptosis (DISC) | 18485876 | |||||
DED | Caspase8 |
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FADD | MTT assay | Apoptosis (DISC) | 22890322 | |||||
DED | Caspase8 |
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FADD | DAPI staining | Apoptosis (DISC) | 9045686 |
(
: click this icon to show interactions only between the two corresponding DDs)
