FADD / Caspase8 (DED)

Interactions between FADD in detail and Caspase8 in detail (DED)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DED FADD Link Caspase8 Fluorescence resonance energy transfer ` 11717445
DED FADD Link Caspase8 GST fusion protein pull-down Not specified Full length E.coli HuH-7 lysate 12407100
DED FADD Link Caspase8 His fusion protein pull-down Not specified Full length Not specified Not specified 1-479 In vitro translation 8681377
DED FADD Link Caspase8 His fusion protein pull-down Not specified Not specified Not specified Human Not specified In vitro translation 9045686
DED FADD Link Caspase8 His fusion protein pull-down Not specified Not specified In vitro translation Human 1-479 In vitro translation 12010809
DED FADD Link Caspase8 In vitro purification protein assembly(2D gel electrophoresis) Not specified Full length HEK293 Not specified 1-479 In vitro translation 8681377
DED FADD Link Caspase8 Yeast two-hybrid Human Full length Yeast Human Not specified Yeast 8681376
DED FADD Link Caspase8 Yeast two-hybrid Human 1-79 Yeast Human Not specified Yeast 8681376
DED FADD Link Caspase8 Yeast two-hybrid Mouse 1-96 Yeast Mouse 1-202 Yeast 10526240
DED FADD Link Caspase8 Yeast two-hybrid Not specified Not specified Yeast Human Full length Yeast 15782135
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DED FADD Link Caspase8 HSC3 21525013
DED FADD Link Caspase8 Co-immunoprecipitation HEK293 Human Not specified Human Not specified 8681376
DED FADD Link Caspase8 Co-immunoprecipitation T47D Human Not specified Not specified Not specified 9582077
DED FADD Link Caspase8 Co-immunoprecipitation Jurkat A3 11432804
DED FADD Link Caspase8 Co-immunoprecipitation MCF-7 Not used Human 1-276 12010809
DED FADD Link Caspase8 Co-immunoprecipitation HEK293 Human 2-208 Human 2-177 12220669
DED FADD Link Caspase8 Co-immunoprecipitation HT1080 12887920
DED FADD Link Caspase8 Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 15701651
DED FADD Link Caspase8 Co-immunoprecipitation SKW6.4 17159907
DED FADD Link Caspase8 Co-immunoprecipitation H2009 18485876
DED FADD Link Caspase8 Co-immunoprecipitation Panc-1 18485876
DED FADD Link Caspase8 Co-immunoprecipitation HT-1080 19060883
DED FADD Link Caspase8 Co-immunoprecipitation HT-29 19524512
DED FADD Link Caspase8 Co-immunoprecipitation H2009 20547836
DED FADD Link Caspase8 Co-immunoprecipitation SH-EP 22890322
DED FADD Link Caspase8 Co-immunoprecipitation LAN-5 22890322
DED FADD Link Caspase8 Fluorescence resonance energy transfer HEK293 Not specified Not specified Not specified Full length 11717445
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DED FADD Link Caspase8 Co-immunoprecipitation F25G, F35V (FADD) 9582077
DED FADD Link Caspase8 Co-immunoprecipitation K33Q and K35Q, F25G (FADD) 12220669
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DED FADD Link Caspase8 β-galactosidase staining Apoptosis (DISC) 8681376
DED FADD Link Caspase8 DNA fragmentation assay Apoptosis (DISC) 11432804
DED FADD Link Caspase8 Propidium iodide staining Apoptosis (DISC) 12010809
DED FADD Link Caspase8 Hoechst staining Apoptosis (DISC) 12887920
DED FADD Link Caspase8 PARP cleaved assay Apoptosis (DISC) 18485876
DED FADD Link Caspase8 MTT assay Apoptosis (DISC) 22890322
DED FADD Link Caspase8 DAPI staining Apoptosis (DISC) 9045686
(Link: click this icon to show interactions only between the two corresponding DDs)