FADD / FLIP (DED)

Interactions between FADD in detail and FLIP in detail (DED)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DED FADD Link FLIP GST fusion protein pull-down Not specified 1-79 In vitro translation Human Full length Not specified 9228018
DED FADD Link FLIP GST fusion protein pull-down Not specified Full length E.coli HuH-7 lysate 12407100
DED FADD Link FLIP Yeast two-hybrid Human Full length Yeast Human 1-215 Yeast 9208847
DED FADD Link FLIP Yeast two-hybrid Not specified 1-79 Yeast Human 1-160 Yeast 9228018
DED FADD Link FLIP Yeast two-hybrid Not specified Not specified Yeast Human Full length Yeast 9289491
DED FADD Link FLIP Yeast two-hybrid Not specified 1-208 In vitro translation Human 1-180 Not specified 9326610
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DED FADD Link FLIP Co-immunoprecipitation HEK293 Not specified Not specified Human 1-195 9208847
DED FADD Link FLIP Co-immunoprecipitation HEK293 Not specified Not specified Human 78-480 9208847
DED FADD Link FLIP Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 9228018
DED FADD Link FLIP Co-immunoprecipitation U937 11859150
DED FADD Link FLIP Co-immunoprecipitation Raji cell Not used Not specified Not specified 12215447
DED FADD Link FLIP Co-immunoprecipitation HEK293 Human 2-208 Human 1-171 12220669
DED FADD Link FLIP Co-immunoprecipitation HEK293 Human 2-208 Human Full length 12220669
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DED FADD Link FLIP Co-immunoprecipitation K33Q and K35Q, F25G, F25Y, K33N and K35N (FADD) 12220669
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DED FADD Link FLIP β-galactosidase staining Apoptosis inhibition 9208847
DED FADD Link FLIP Annexin V staining Apoptosis inhibition 11859150
DED FADD Link FLIP Confocal microscopy Apoptosis inhibition 12215447
(Link: click this icon to show interactions only between the two corresponding DDs)