FLIP / FADD (DED)

Interactions between FLIP in detail and FADD in detail (DED)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DED FLIP Link FADD GST fusion protein pull-down Human Full length Not specified Not specified 1-79 In vitro translation 9228018
DED FLIP Link FADD GST fusion protein pull-down HuH-7 lysate Not specified Full length E.coli 12407100
DED FLIP Link FADD Yeast two-hybrid Human 1-215 Yeast Human Full length Yeast 9208847
DED FLIP Link FADD Yeast two-hybrid Human 1-160 Yeast Not specified 1-79 Yeast 9228018
DED FLIP Link FADD Yeast two-hybrid Human Full length Yeast Not specified Not specified Yeast 9289491
DED FLIP Link FADD Yeast two-hybrid Human 1-180 Not specified Not specified 1-208 In vitro translation 9326610
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DED FLIP Link FADD Co-immunoprecipitation HEK293 Human 1-195 Not specified Not specified 9208847
DED FLIP Link FADD Co-immunoprecipitation HEK293 Human 78-480 Not specified Not specified 9208847
DED FLIP Link FADD Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 9228018
DED FLIP Link FADD Co-immunoprecipitation U937 11859150
DED FLIP Link FADD Co-immunoprecipitation Raji cell Not specified Not specified Not used 12215447
DED FLIP Link FADD Co-immunoprecipitation HEK293 Human 1-171 Human 2-208 12220669
DED FLIP Link FADD Co-immunoprecipitation HEK293 Human Full length Human 2-208 12220669
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DED FLIP Link FADD Co-immunoprecipitation K33Q and K35Q, F25G, F25Y, K33N and K35N (FADD) 12220669
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DED FLIP Link FADD β-galactosidase staining Apoptosis inhibition 9208847
DED FLIP Link FADD Annexin V staining Apoptosis inhibition 11859150
DED FLIP Link FADD Confocal microscopy Apoptosis inhibition 12215447
(Link: click this icon to show interactions only between the two corresponding DDs)