ASC / NLRP3 (PYD)

Interactions between ASC in detail and NLRP3 in detail (PYD)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
PYD ASC Link NLRP3 Co-immunoprecipitation THP-1 lysate Not specified 1-112 HEK293 15030775
PYD ASC Link NLRP3 GST fusion protein pull-down Not specified 1-91 E.coli Not specified Full length In vitro translation 23066025
PYD ASC Link NLRP3 Two-hybrid Not specified 1-110 HEK293 Not specified 1-116 HEK293 11786556
PYD ASC Link NLRP3 Yeast two-hybrid Human Full length Yeast Human 1-101 Yeast 19337385
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
PYD ASC Link NLRP3 Co-Immunofluorescence staining HEK293 Not specified Not specified Not specified Full length 11786556
PYD ASC Link NLRP3 Co-Immunofluorescence staining HEK293 Not specified Not specified Not specified 1-739 11786556
PYD ASC Link NLRP3 Co-Immunofluorescence staining HEK293 Human Full length Not specified Full length 16037825
PYD ASC Link NLRP3 Co-immunoprecipitation HEK293 Human Not specified Human 1-96 12615073
PYD ASC Link NLRP3 Co-immunoprecipitation HEK293 Not specified Not specified Not specified 1-112 15030775
PYD ASC Link NLRP3 Co-immunoprecipitation THP-1 15030775
PYD ASC Link NLRP3 Co-immunoprecipitation HEK293 Not specified Not specified Human 1-112 15030775
PYD ASC Link NLRP3 Co-immunoprecipitation HEK293 Human Not specified Not specified 1-87 19158675
PYD ASC Link NLRP3 Co-immunoprecipitation HMVEC-d 21575908
PYD ASC Link NLRP3 Co-immunoprecipitation HuH-7.5 21994322
PYD ASC Link NLRP3 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 22361007
PYD ASC Link NLRP3 Co-immunoprecipitation HEK293 Not specified 1-91 Not specified Full length 23066025
PYD ASC Link NLRP3 Fluorescence resonance energy transfer HEK293 Human Not specified Not specified 1-87 19158675
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
PYD ASC Link NLRP3 Co-immunoprecipitation E13A, K21A, R41A, D48A, D51A (ASC) 23066025
PYD ASC Link NLRP3 GST fusion protein pull-down E13A, K21A, R41A, D48A, D51A (ASC) 23066025
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
PYD ASC Link NLRP3 Luciferase assay NF-kB activation (Inflammasome) 11786556
PYD ASC Link NLRP3 Caspase cleaved assay Caspase1 activation (Inflammasome) 16037825
PYD ASC Link NLRP3 Luciferase assay NF-kB activation (Inflammasome) 12615073
PYD ASC Link NLRP3 Caspase cleaved assay Caspase1 activation (Inflammasome) 15030775
(Link: click this icon to show interactions only between the two corresponding DDs)