ASC / POP1 (PYD)

Interactions between ASC in detail and POP1 in detail (PYD)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
PYD ASC Link POP1 GST fusion protein pull-down Not specified 1-100 E.coli Human Not specified In vitro translation 12656673
PYD ASC Link POP1 GST fusion protein pull-down Not specified 1-91 E.coli Not specified 1-89 In vitro translation 23066025
PYD ASC Link POP1 In vitro purification protein assembly(Surface plasmon resonance) Not specified 1-96 E.coli Not specified 1-89 E.coli 18362139
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
PYD ASC Link POP1 Co-Immunofluorescence staining COS-7 Not specified Not specified Not specified Not specified 12656673
PYD ASC Link POP1 Co-immunoprecipitation HEK293 Not specified 1-100 Human Not specified 12656673
PYD ASC Link POP1 Co-immunoprecipitation HEK293 Human Not specified Human Not specified 17178784
PYD ASC Link POP1 Co-immunoprecipitation HEK293 Not specified 1-91 Not specified 1-89 23066025
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
PYD ASC Link POP1 Co-immunoprecipitation E13A, K21A, R41A, D48A, D51A (ASC) 23066025
PYD ASC Link POP1 GST fusion protein pull-down R3A, D10A, E13A, K21A, R38A, R41A, D48A, D51A, D54A, Q84A (ASC) 23066025
PYD ASC Link POP1 Surface plasmon resonance 1:1 4.8μM D6A and L25A, E13A and L25A, K21A, Y36A, D48A and L25A, D54A and L25A (ASC) 18362139
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
PYD ASC Link POP1 Luciferase assay NF-kB activation block 12656673
(Link: click this icon to show interactions only between the two corresponding DDs)