NLRP3 / ASC (PYD)

Interactions between NLRP3 in detail and ASC in detail (PYD)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
PYD NLRP3 Link ASC Co-immunoprecipitation Not specified 1-112 HEK293 THP-1 lysate 15030775
PYD NLRP3 Link ASC GST fusion protein pull-down Not specified Full length In vitro translation Not specified 1-91 E.coli 23066025
PYD NLRP3 Link ASC Two-hybrid Not specified 1-116 HEK293 Not specified 1-110 HEK293 11786556
PYD NLRP3 Link ASC Yeast two-hybrid Human 1-101 Yeast Human Full length Yeast 19337385
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
PYD NLRP3 Link ASC Co-Immunofluorescence staining HEK293 Not specified Full length Not specified Not specified 11786556
PYD NLRP3 Link ASC Co-Immunofluorescence staining HEK293 Not specified 1-739 Not specified Not specified 11786556
PYD NLRP3 Link ASC Co-Immunofluorescence staining HEK293 Not specified Full length Human Full length 16037825
PYD NLRP3 Link ASC Co-immunoprecipitation HEK293 Human 1-96 Human Not specified 12615073
PYD NLRP3 Link ASC Co-immunoprecipitation HEK293 Not specified 1-112 Not specified Not specified 15030775
PYD NLRP3 Link ASC Co-immunoprecipitation THP-1 15030775
PYD NLRP3 Link ASC Co-immunoprecipitation HEK293 Human 1-112 Not specified Not specified 15030775
PYD NLRP3 Link ASC Co-immunoprecipitation HEK293 Not specified 1-87 Human Not specified 19158675
PYD NLRP3 Link ASC Co-immunoprecipitation HMVEC-d 21575908
PYD NLRP3 Link ASC Co-immunoprecipitation HuH-7.5 21994322
PYD NLRP3 Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 22361007
PYD NLRP3 Link ASC Co-immunoprecipitation HEK293 Not specified Full length Not specified 1-91 23066025
PYD NLRP3 Link ASC Fluorescence resonance energy transfer HEK293 Not specified 1-87 Human Not specified 19158675
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
PYD NLRP3 Link ASC Co-immunoprecipitation E13A, K21A, R41A, D48A, D51A (ASC) 23066025
PYD NLRP3 Link ASC GST fusion protein pull-down E13A, K21A, R41A, D48A, D51A (ASC) 23066025
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
PYD NLRP3 Link ASC Luciferase assay NF-kB activation (Inflammasome) 11786556
PYD NLRP3 Link ASC Caspase cleaved assay Caspase1 activation (Inflammasome) 16037825
PYD NLRP3 Link ASC Luciferase assay NF-kB activation (Inflammasome) 12615073
PYD NLRP3 Link ASC Caspase cleaved assay Caspase1 activation (Inflammasome) 15030775
(Link: click this icon to show interactions only between the two corresponding DDs)