PYD

Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
PYD AIM2 Link AIM2 In vitro purification protein assembly(chemical crosslinking) Human Full length Not specified Human Full length Not specified 19158676
PYD AIM2 Link ASC GST fusion protein pull-down Human Full length Not specified Human Full length Not specified 19158676
PYD AIM2 Link ASC GST fusion protein pull-down Human Full length Not specified Human Not specified Not specified 19158676
PYD ASC Link AIM2 GST fusion protein pull-down Human Full length Not specified Human Full length Not specified 19158676
PYD ASC Link AIM2 GST fusion protein pull-down Human Not specified Not specified Human Full length Not specified 19158676
PYD ASC Link ASC Co-immunoprecipitation Not specified Not specified HEK293 THP-1 lysate 15030775
PYD ASC Link ASC GST fusion protein pull-down Human 1-100 E.coli Human 1-100 In vitro translation 12656673
PYD ASC Link ASC GST fusion protein pull-down Not specified 1-91 E.coli Not specified 1-96 In vitro translation 23066025
PYD ASC Link ASC Yeast two-hybrid Human 1-94 Yeast Human 1-94 Yeast 19337385
PYD ASC Link NLRP2 Co-immunoprecipitation THP-1 lysate Human 1-93 HEK293 15030775
PYD ASC Link NLRP3 Co-immunoprecipitation THP-1 lysate Not specified 1-112 HEK293 15030775
PYD ASC Link NLRP3 GST fusion protein pull-down Not specified 1-91 E.coli Not specified Full length In vitro translation 23066025
PYD ASC Link NLRP3 Two-hybrid Not specified 1-110 HEK293 Not specified 1-116 HEK293 11786556
PYD ASC Link NLRP3 Yeast two-hybrid Human Full length Yeast Human 1-101 Yeast 19337385
PYD ASC Link POP1 GST fusion protein pull-down Not specified 1-100 E.coli Human Not specified In vitro translation 12656673
PYD ASC Link POP1 GST fusion protein pull-down Not specified 1-91 E.coli Not specified 1-89 In vitro translation 23066025
PYD ASC Link POP1 In vitro purification protein assembly(Surface plasmon resonance) Not specified 1-96 E.coli Not specified 1-89 E.coli 18362139
PYD ASC Link POP2 GST fusion protein pull-down Human Not specified In vitro translation Not specified Human E.coli 17178784
PYD ASC Link POP2 GST fusion protein pull-down Not specified Human In vitro translation Human Not specified E.coli 17178784
PYD ASC Link POP2 Yeast two-hybrid Human Not specified Yeast Human Not specified Yeast 17178784
PYD ASC Link Pyrin His fusion protein pull-down Human Full length HEK293 Not specified Full length Sf-9 16037825
PYD NLRP1 Link POP2 Yeast two-hybrid Not specified Not specified Yeast Human Not specified Yeast 17178784
PYD NLRP2 Link ASC Co-immunoprecipitation Human 1-93 HEK293 THP-1 lysate 15030775
PYD NLRP2 Link POP2 GST fusion protein pull-down Not specified Not specified In vitro translation Not specified Human E.coli 17178784
PYD NLRP2 Link POP2 GST fusion protein pull-down Not specified Not specified In vitro translation Human Not specified E.coli 17178784
PYD NLRP2 Link POP2 Yeast two-hybrid Not specified Not specified Yeast Human Not specified Yeast 17178784
PYD NLRP3 Link ASC Co-immunoprecipitation Not specified 1-112 HEK293 THP-1 lysate 15030775
PYD NLRP3 Link ASC GST fusion protein pull-down Not specified Full length In vitro translation Not specified 1-91 E.coli 23066025
PYD NLRP3 Link ASC Two-hybrid Not specified 1-116 HEK293 Not specified 1-110 HEK293 11786556
PYD NLRP3 Link ASC Yeast two-hybrid Human 1-101 Yeast Human Full length Yeast 19337385
PYD POP1 Link ASC GST fusion protein pull-down Human Not specified In vitro translation Not specified 1-100 E.coli 12656673
PYD POP1 Link ASC GST fusion protein pull-down Not specified 1-89 In vitro translation Not specified 1-91 E.coli 23066025
PYD POP1 Link ASC In vitro purification protein assembly(Surface plasmon resonance) Not specified 1-89 E.coli Not specified 1-96 E.coli 18362139
PYD POP2 Link ASC GST fusion protein pull-down Not specified Human E.coli Human Not specified In vitro translation 17178784
PYD POP2 Link ASC GST fusion protein pull-down Human Not specified E.coli Not specified Human In vitro translation 17178784
PYD POP2 Link ASC Yeast two-hybrid Human Not specified Yeast Human Not specified Yeast 17178784
PYD POP2 Link NLRP1 Yeast two-hybrid Human Not specified Yeast Not specified Not specified Yeast 17178784
PYD POP2 Link NLRP2 GST fusion protein pull-down Not specified Human E.coli Not specified Not specified In vitro translation 17178784
PYD POP2 Link NLRP2 GST fusion protein pull-down Human Not specified E.coli Not specified Not specified In vitro translation 17178784
PYD POP2 Link NLRP2 Yeast two-hybrid Human Not specified Yeast Not specified Not specified Yeast 17178784
PYD Pyrin Link ASC His fusion protein pull-down Not specified Full length Sf-9 Human Full length HEK293 16037825
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
PYD AIM2 Link ASC Co-immunoprecipitation HEK293 Human 1-83 Human Not specified 19158675
PYD AIM2 Link ASC Co-immunoprecipitation THP-1 19158676
PYD AIM2 Link ASC Co-immunoprecipitation HMVEC-d 21575908
PYD AIM2 Link ASC Co-immunoprecipitation NPC TW02 Human Full length Human Full length 22869553
PYD AIM2 Link ASC Fluorescence resonance energy transfer HEK293 Human Full length Human Not specified 19158675
PYD AIM2 Link ASC Fluorescence resonance energy transfer HEK293 Human 1-83 Human Not specified 19158675
PYD ASC Link AIM2 Co-immunoprecipitation HEK293 Human Not specified Human 1-83 19158675
PYD ASC Link AIM2 Co-immunoprecipitation THP-1 19158676
PYD ASC Link AIM2 Co-immunoprecipitation HMVEC-d 21575908
PYD ASC Link AIM2 Co-immunoprecipitation NPC TW02 Human Full length Human Full length 22869553
PYD ASC Link AIM2 Fluorescence resonance energy transfer HEK293 Human Not specified Human Full length 19158675
PYD ASC Link AIM2 Fluorescence resonance energy transfer HEK293 Human Not specified Human 1-83 19158675
PYD ASC Link ASC Co-immunoprecipitation COS-7 Not specified 101-195 Not specified 101-195 11162571
PYD ASC Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 15030775
PYD ASC Link ASC Co-immunoprecipitation HEK293 Not specified 1-91 Not specified Full length 23066025
PYD ASC Link ASC Co-immunoprecipitation HEK293 Not specified 1-91 Not specified 1-96 23066025
PYD ASC Link IFI16 Co-Immunofluorescence staining HMVEC-d 21575908
PYD ASC Link IFI16 Co-immunoprecipitation HMVEC-d 21575908
PYD ASC Link NLRP1 Co-immunoprecipitation THP-1 17418785
PYD ASC Link NLRP1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 17418785
PYD ASC Link NLRP1 Co-immunoprecipitation Rat cortical lysate 19401709
PYD ASC Link NLRP1 Size-exclusion chromatography Mice cerebral cortex 19066616
PYD ASC Link NLRP10 Co-Immunofluorescence staining HEK293 20393137
PYD ASC Link NLRP10 Co-immunoprecipitation HEK293 Human Not specified Human Not specified 15096476
PYD ASC Link NLRP12 Co-Immunofluorescence staining HEK293 Not specified Not specified Not specified Full length 12019269
PYD ASC Link NLRP2 Co-immunoprecipitation HEK293 Not specified Not specified Human 1-93 15030775
PYD ASC Link NLRP2 Co-immunoprecipitation THP-1 Not used Human Full length 15030775
PYD ASC Link NLRP2 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 15456791
PYD ASC Link NLRP2 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Full length 15456791
PYD ASC Link NLRP3 Co-Immunofluorescence staining HEK293 Not specified Not specified Not specified Full length 11786556
PYD ASC Link NLRP3 Co-Immunofluorescence staining HEK293 Not specified Not specified Not specified 1-739 11786556
PYD ASC Link NLRP3 Co-Immunofluorescence staining HEK293 Human Full length Not specified Full length 16037825
PYD ASC Link NLRP3 Co-immunoprecipitation HEK293 Human Not specified Human 1-96 12615073
PYD ASC Link NLRP3 Co-immunoprecipitation HEK293 Not specified Not specified Not specified 1-112 15030775
PYD ASC Link NLRP3 Co-immunoprecipitation THP-1 15030775
PYD ASC Link NLRP3 Co-immunoprecipitation HEK293 Not specified Not specified Human 1-112 15030775
PYD ASC Link NLRP3 Co-immunoprecipitation HEK293 Human Not specified Not specified 1-87 19158675
PYD ASC Link NLRP3 Co-immunoprecipitation HMVEC-d 21575908
PYD ASC Link NLRP3 Co-immunoprecipitation HuH-7.5 21994322
PYD ASC Link NLRP3 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 22361007
PYD ASC Link NLRP3 Co-immunoprecipitation HEK293 Not specified 1-91 Not specified Full length 23066025
PYD ASC Link NLRP3 Fluorescence resonance energy transfer HEK293 Human Not specified Not specified 1-87 19158675
PYD ASC Link NLRP7 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 22361007
PYD ASC Link POP1 Co-Immunofluorescence staining COS-7 Not specified Not specified Not specified Not specified 12656673
PYD ASC Link POP1 Co-immunoprecipitation HEK293 Not specified 1-100 Human Not specified 12656673
PYD ASC Link POP1 Co-immunoprecipitation HEK293 Human Not specified Human Not specified 17178784
PYD ASC Link POP1 Co-immunoprecipitation HEK293 Not specified 1-91 Not specified 1-89 23066025
PYD ASC Link POP2 Co-Immunofluorescence staining HEK293 Human Not specified Human Not specified 17178784
PYD ASC Link POP2 Co-Immunofluorescence staining HeLa Not specified Not specified Human Not specified 17339483
PYD ASC Link POP2 Co-immunoprecipitation HEK293 Human Not specified Human Not specified 17178784
PYD ASC Link Pyrin Co-Immunofluorescence staining HeLa Not specified Not specified Not specified Not specified 11498534
PYD ASC Link Pyrin Co-Immunofluorescence staining PT67 Mouse Full length Mouse Full length 12667444
PYD ASC Link Pyrin Co-immunoprecipitation HEK293 Human Not specified Not specified Full length 12615073
PYD ASC Link Pyrin Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 16037825
PYD ASC Link Pyrin GST fusion protein pull-down PT67 Mouse Full length Mouse Not specified 12667444
PYD IFI16 Link ASC Co-Immunofluorescence staining HMVEC-d 21575908
PYD IFI16 Link ASC Co-immunoprecipitation HMVEC-d 21575908
PYD NLRP1 Link ASC Co-immunoprecipitation THP-1 17418785
PYD NLRP1 Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 17418785
PYD NLRP1 Link ASC Co-immunoprecipitation Rat cortical lysate 19401709
PYD NLRP1 Link ASC Size-exclusion chromatography Mice cerebral cortex 19066616
PYD NLRP1 Link NLRP2 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Full length 15456791
PYD NLRP10 Link ASC Co-Immunofluorescence staining HEK293 20393137
PYD NLRP10 Link ASC Co-immunoprecipitation HEK293 Human Not specified Human Not specified 15096476
PYD NLRP10 Link NLRP10 Co-immunoprecipitation HEK293 Human Not specified Human Not specified 15096476
PYD NLRP12 Link ASC Co-Immunofluorescence staining HEK293 Not specified Full length Not specified Not specified 12019269
PYD NLRP2 Link ASC Co-immunoprecipitation HEK293 Human 1-93 Not specified Not specified 15030775
PYD NLRP2 Link ASC Co-immunoprecipitation THP-1 Human Full length Not used 15030775
PYD NLRP2 Link ASC Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 15456791
PYD NLRP2 Link ASC Co-immunoprecipitation HEK293 Not specified Full length Not specified Not specified 15456791
PYD NLRP2 Link NLRP1 Co-immunoprecipitation HEK293 Not specified Full length Not specified Not specified 15456791
PYD NLRP2 Link POP2 Co-Immunofluorescence staining HEK293 Not specified Not specified Human Not specified 17178784
PYD NLRP2 Link POP2 Co-immunoprecipitation HEK293 Not specified Not specified Human Not specified 17178784
PYD NLRP2 Link POP2 Co-immunoprecipitation HEK293 Not specified Not specified Not used Not specified 17178784
PYD NLRP3 Link ASC Co-Immunofluorescence staining HEK293 Not specified Full length Not specified Not specified 11786556
PYD NLRP3 Link ASC Co-Immunofluorescence staining HEK293 Not specified 1-739 Not specified Not specified 11786556
PYD NLRP3 Link ASC Co-Immunofluorescence staining HEK293 Not specified Full length Human Full length 16037825
PYD NLRP3 Link ASC Co-immunoprecipitation HEK293 Human 1-96 Human Not specified 12615073
PYD NLRP3 Link ASC Co-immunoprecipitation HEK293 Not specified 1-112 Not specified Not specified 15030775
PYD NLRP3 Link ASC Co-immunoprecipitation THP-1 15030775
PYD NLRP3 Link ASC Co-immunoprecipitation HEK293 Human 1-112 Not specified Not specified 15030775
PYD NLRP3 Link ASC Co-immunoprecipitation HEK293 Not specified 1-87 Human Not specified 19158675
PYD NLRP3 Link ASC Co-immunoprecipitation HMVEC-d 21575908
PYD NLRP3 Link ASC Co-immunoprecipitation HuH-7.5 21994322
PYD NLRP3 Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 22361007
PYD NLRP3 Link ASC Co-immunoprecipitation HEK293 Not specified Full length Not specified 1-91 23066025
PYD NLRP3 Link ASC Fluorescence resonance energy transfer HEK293 Not specified 1-87 Human Not specified 19158675
PYD NLRP3 Link NLRP3 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 17483456
PYD NLRP3 Link NLRP3 Size-exclusion chromatography HEK293 Not specified Full length Not specified Full length 17483456
PYD NLRP7 Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 22361007
PYD POP1 Link ASC Co-Immunofluorescence staining COS-7 Not specified Not specified Not specified Not specified 12656673
PYD POP1 Link ASC Co-immunoprecipitation HEK293 Human Not specified Not specified 1-100 12656673
PYD POP1 Link ASC Co-immunoprecipitation HEK293 Human Not specified Human Not specified 17178784
PYD POP1 Link ASC Co-immunoprecipitation HEK293 Not specified 1-89 Not specified 1-91 23066025
PYD POP2 Link ASC Co-Immunofluorescence staining HEK293 Human Not specified Human Not specified 17178784
PYD POP2 Link ASC Co-Immunofluorescence staining HeLa Human Not specified Not specified Not specified 17339483
PYD POP2 Link ASC Co-immunoprecipitation HEK293 Human Not specified Human Not specified 17178784
PYD POP2 Link NLRP2 Co-Immunofluorescence staining HEK293 Human Not specified Not specified Not specified 17178784
PYD POP2 Link NLRP2 Co-immunoprecipitation HEK293 Human Not specified Not specified Not specified 17178784
PYD POP2 Link NLRP2 Co-immunoprecipitation HEK293 Not used Not specified Not specified Not specified 17178784
PYD Pyrin Link ASC Co-Immunofluorescence staining HeLa Not specified Not specified Not specified Not specified 11498534
PYD Pyrin Link ASC Co-Immunofluorescence staining PT67 Mouse Full length Mouse Full length 12667444
PYD Pyrin Link ASC Co-immunoprecipitation HEK293 Not specified Full length Human Not specified 12615073
PYD Pyrin Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 16037825
PYD Pyrin Link ASC GST fusion protein pull-down PT67 Mouse Not specified Mouse Full length 12667444
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
PYD ASC Link ASC Co-immunoprecipitation E13A, K21A, R41A, D48A, D51A (ASC) 23066025
PYD ASC Link ASC GST fusion protein pull-down E13A, K21A, R41A, D48A, D51A (ASC) 23066025
PYD ASC Link NLRP2 Co-immunoprecipitation E13W (ASC) 15456791
PYD ASC Link NLRP3 Co-immunoprecipitation E13A, K21A, R41A, D48A, D51A (ASC) 23066025
PYD ASC Link NLRP3 GST fusion protein pull-down E13A, K21A, R41A, D48A, D51A (ASC) 23066025
PYD ASC Link POP1 Co-immunoprecipitation E13A, K21A, R41A, D48A, D51A (ASC) 23066025
PYD ASC Link POP1 GST fusion protein pull-down R3A, D10A, E13A, K21A, R38A, R41A, D48A, D51A, D54A, Q84A (ASC) 23066025
PYD ASC Link POP1 Surface plasmon resonance 1:1 4.8μM D6A and L25A, E13A and L25A, K21A, Y36A, D48A and L25A, D54A and L25A (ASC) 18362139
PYD NLRP2 Link ASC Co-immunoprecipitation E13W (ASC) 15456791
PYD NLRP3 Link ASC Co-immunoprecipitation E13A, K21A, R41A, D48A, D51A (ASC) 23066025
PYD NLRP3 Link ASC GST fusion protein pull-down E13A, K21A, R41A, D48A, D51A (ASC) 23066025
PYD POP1 Link ASC Co-immunoprecipitation E13A, K21A, R41A, D48A, D51A (ASC) 23066025
PYD POP1 Link ASC GST fusion protein pull-down R3A, D10A, E13A, K21A, R38A, R41A, D48A, D51A, D54A, Q84A (ASC) 23066025
PYD POP1 Link ASC Surface plasmon resonance 1:1 4.8μM D6A and L25A, E13A and L25A, K21A, Y36A, D48A and L25A, D54A and L25A (ASC) 18362139
PYD POP1 Link ASC Surface plasmon resonance 1:1 4.8μM D6A and L25A, E13A and L25A, K21A, Y36A, D48A and L25A, D54A and L25A (ASC) 18362139
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
PYD AIM2 Link ASC Luciferase assay NF-kB activation (Inflammasome) 19158675
PYD AIM2 Link ASC Caspase cleaved assay Caspase1 activation (Inflammasome) 19158676
PYD ASC Link AIM2 Luciferase assay NF-kB activation (Inflammasome) 19158675
PYD ASC Link AIM2 Caspase cleaved assay Caspase1 activation (Inflammasome) 19158676
PYD ASC Link NLRP10 Propidium iodide-Annexin V staining, Luciferase assay, Immunostaining Apoptosis inhibition NF-kB-activation block, ASC aggregation inhibition 20393137
PYD ASC Link NLRP10 Annexin V staining, EMSA ASC mediated Apoptosis block NF-kB-activation block 15096476
PYD ASC Link NLRP12 Luciferase assay, ELISA NF-kB activation, IL-1b secretion 12019269
PYD ASC Link NLRP2 ELISA IL-1b secretion (Inflammasome) 15456791
PYD ASC Link NLRP3 Luciferase assay NF-kB activation (Inflammasome) 11786556
PYD ASC Link NLRP3 Caspase cleaved assay Caspase1 activation (Inflammasome) 16037825
PYD ASC Link NLRP3 Luciferase assay NF-kB activation (Inflammasome) 12615073
PYD ASC Link NLRP3 Caspase cleaved assay Caspase1 activation (Inflammasome) 15030775
PYD ASC Link POP1 Luciferase assay NF-kB activation block 12656673
PYD ASC Link POP2 ELISA IL-1b secretion decrease 17178784
PYD ASC Link POP2 Immunofluorescence staining Inflammasome inhibition 17339483
PYD ASC Link Pyrin DAPI staining Apoptosis inhibition 11498534
PYD ASC Link Pyrin Apoptotic DNA ladder assay Apoptosis inhibition 12667444
PYD ASC Link Pyrin β-galactosidase staining, Luciferase assay Apoptosis inhibition NLRP3-ASC mediated NF-kB activation block 12615073
PYD ASC Link Pyrin Caspase cleaved assay Caspase1 activation (Inflammasome) 16037825
PYD NLRP10 Link ASC Propidium iodide-Annexin V staining, Luciferase assay, Immunostaining Apoptosis inhibition NF-kB-activation block, ASC aggregation inhibition 20393137
PYD NLRP10 Link ASC Annexin V staining, EMSA ASC mediated Apoptosis block NF-kB-activation block 15096476
PYD NLRP12 Link ASC Luciferase assay, ELISA NF-kB activation, IL-1b secretion 12019269
PYD NLRP2 Link ASC ELISA IL-1b secretion (Inflammasome) 15456791
PYD NLRP2 Link POP2 ELISA IL-1b secretion decrease 17178784
PYD NLRP3 Link ASC Luciferase assay NF-kB activation (Inflammasome) 11786556
PYD NLRP3 Link ASC Caspase cleaved assay Caspase1 activation (Inflammasome) 16037825
PYD NLRP3 Link ASC Luciferase assay NF-kB activation (Inflammasome) 12615073
PYD NLRP3 Link ASC Caspase cleaved assay Caspase1 activation (Inflammasome) 15030775
PYD POP1 Link ASC Luciferase assay NF-kB activation block 12656673
PYD POP2 Link ASC ELISA IL-1b secretion decrease 17178784
PYD POP2 Link ASC Immunofluorescence staining Inflammasome inhibition 17339483
PYD POP2 Link NLRP2 ELISA IL-1b secretion decrease 17178784
PYD Pyrin Link ASC DAPI staining Apoptosis inhibition 11498534
PYD Pyrin Link ASC Apoptotic DNA ladder assay Apoptosis inhibition 12667444
PYD Pyrin Link ASC β-galactosidase staining, Luciferase assay Apoptosis inhibition NLRP3-ASC mediated NF-kB activation block 12615073
PYD Pyrin Link ASC Caspase cleaved assay Caspase1 activation (Inflammasome) 16037825
(Link: click this icon to show interactions only between the two corresponding DDs)