ASC (CARD)

Full Name:
Apoptosis-associated speck-like protein containing a CARD
Alternative Names:
TMS, TMS1, CARD5, TMS-1, MGC10332, PYCARD
Function:
Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.
Death Domain Subfamily & Boundary Region:
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq Q9ULZ3external db link DIP-27618Nexternal db link Q9ULZ3external db link hsa:29108external db link Q9ULZ3external db link MINT-206960external db link
Mouse Seq Q9EPB4external db link DIP-27619Nexternal db link Q9EPB4external db link mmu:66824external db link
Rat Seq Q8CHK8external db link Q8CHK8external db link rno:282817external db link
Dog Seq E2RQ18external db link cfa:489925external db link
Zebra fish Seq Q9I9N6external db link Q9I9N6external db link
Structure Species Residue Method Expression Purification Column Final condition Final concentration Reference
2KN6 Download Human 1-195 NMR E.coli Ion Exchange 1mM 19759015
No information
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
CARD ASC Link ASC GST fusion protein pull-down Not specified Not specified E.coli Not specified Not specified In vitro translation 14634131
CARD ASC Link ASC Yeast two-hybrid Human 105-195 Yeast Human 105-195 Yeast 19337385
CARD ASC Link CARD12 GST fusion protein pull-down Human 105-195 In vitro translation Human 1-88 E.coli 11967258
CARD ASC Link CARD12 GST fusion protein pull-down Human Full length In vitro translation Human 1-88 E.coli 11967258
CARD ASC Link CARD12 Two-hybrid Not specified Not specified HEK293 Not specified 1-83 HEK293 11374873
CARD ASC Link CARD12 Yeast two-hybrid Human Full length Yeast Human 1-96 Yeast 19337385
CARD ASC Link CARD16 GST fusion protein pull-down Human 105-195 In vitro translation Human Full length E.coli 11967258
CARD ASC Link CARD16 GST fusion protein pull-down Human Full length In vitro translation Human Full length E.coli 11967258
CARD ASC Link Caspase1 GST fusion protein pull-down Human 105-195 In vitro translation Not specified Not specified E.coli 11967258
CARD ASC Link Caspase1 GST fusion protein pull-down Human Full length In vitro translation Not specified Not specified E.coli 11967258
CARD ASC Link Caspase1 GST fusion protein pull-down Not specified Not specified E.coli Not specified Not specified In vitro translation 14634131
CARD ASC Link Caspase1 Yeast two-hybrid Human 105-195 Yeast Human 1-91 Yeast 19337385
(Link: click this icon to show interactions only between the two corresponding DDs)
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In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD ASC Link ASC Co-immunoprecipitation COS-7 Not specified 1-100 Not specified 1-100 11162571
CARD ASC Link CARD12 Co-immunoprecipitation HEK293 Not specified Full length Not specified 1-647 12646168
CARD ASC Link CARD12 Co-immunoprecipitation HEK293 Human Not specified Human 1-457 15701651
CARD ASC Link Caspase1 Co-immunoprecipitation HEK293 Human 105-195 Not specified Not specified 11967258
CARD ASC Link Caspase1 Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 11967258
CARD ASC Link Caspase1 Co-immunoprecipitation HEK293 Not specified 92-195 Not specified Not specified 12019269
CARD ASC Link caspase1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 12191486
CARD ASC Link Caspase1 Co-immunoprecipitation PT67 Mouse Full length Mouse Not specified 12667444
CARD ASC Link Caspase1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 14634131
CARD ASC Link Caspase1 Co-immunoprecipitation THP-1 14634131
CARD ASC Link Caspase1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 16585594
CARD ASC Link Caspase1 Co-immunoprecipitation THP-1 16585594
CARD ASC Link Caspase1 Co-immunoprecipitation Rat cortical lysate 19401709
CARD ASC Link Caspase1 Co-immunoprecipitation HEK239 Human Not specified Human Not specified 21862576
CARD ASC Link Caspase1 GST fusion protein pull-down PT67 Mouse Full length Mouse Not specified 12667444
CARD ASC Link Caspase1 Size-exclusion chromatography Mice cerebral cortex 19066616
CARD ASC Link RIPK2 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 14634131
(Link: click this icon to show interactions only between the two corresponding DDs)
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Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
CARD ASC Link Caspase1 Co-immunoprecipitation L122Q(ASC) 14634131
CARD ASC Link Caspase1 Co-immunoprecipitation D27G, R45D (Caspase1) 21862576
CARD ASC Link RIPK2 Co-immunoprecipitation L122Q(ASC) 14634131
(Link: click this icon to show interactions only between the two corresponding DDs)
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Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
CARD ASC Link CARD12 β-galactosidase staining, Luciferase assay Apoptosis NF-kB activation 12646168
CARD ASC Link Caspase1 ELISA IL-1 secretion (Inflammasome) 11967258
CARD ASC Link Caspase1 ELISA IL-1b secretion (Inflammasome) 12019269
CARD ASC Link Caspase1 ELISA IL-1b secretion decrease 14634131
CARD ASC Link Caspase1 ELISA IL-1b secretion 16585594
CARD ASC Link Caspase1 Luciferase assay NF-kB activation 21862576
CARD ASC Link RIPK2 Immunoprecipitation Pro-caspase1-RIPK2 complex inhibition 14634131
(Link: click this icon to show interactions only between the two corresponding DDs)