ASC (PYD)

Full Name:
Apoptosis-associated speck-like protein containing a CARD
Alternative Names:
TMS, TMS1, CARD5, TMS-1, MGC10332, PYCARD
Function:
Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.
Death Domain Subfamily & Boundary Region:
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq Q9ULZ3external db link DIP-27618Nexternal db link Q9ULZ3external db link hsa:29108external db link Q9ULZ3external db link MINT-206960external db link
Mouse Seq Q9EPB4external db link DIP-27619Nexternal db link Q9EPB4external db link mmu:66824external db link
Rat Seq Q8CHK8external db link Q8CHK8external db link rno:282817external db link
Dog Seq E2RQ18external db link cfa:489925external db link
Zebra fish Seq Q9I9N6external db link Q9I9N6external db link
Structure Species Residue Method Expression Purification Column Final condition Final concentration Reference
1UCP Download Human 1-91 NMR E.coli Affinity chromatography Phosphate buffered saline(pH3.7), 1% triton X-100, 1mM DTT, 10% glycerol, 50mM glycine 1mg/ml 14499617
2KN6 Download Human 1-195 NMR E.coli Ion Exchange 1mM 19759015
No information
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
PYD ASC Link AIM2 GST fusion protein pull-down Human Full length Not specified Human Full length Not specified 19158676
PYD ASC Link AIM2 GST fusion protein pull-down Human Not specified Not specified Human Full length Not specified 19158676
PYD ASC Link ASC Co-immunoprecipitation Not specified Not specified HEK293 THP-1 lysate 15030775
PYD ASC Link ASC GST fusion protein pull-down Human 1-100 E.coli Human 1-100 In vitro translation 12656673
PYD ASC Link ASC GST fusion protein pull-down Not specified 1-91 E.coli Not specified 1-96 In vitro translation 23066025
PYD ASC Link ASC Yeast two-hybrid Human 1-94 Yeast Human 1-94 Yeast 19337385
PYD ASC Link NLRP2 Co-immunoprecipitation THP-1 lysate Human 1-93 HEK293 15030775
PYD ASC Link NLRP3 Co-immunoprecipitation THP-1 lysate Not specified 1-112 HEK293 15030775
PYD ASC Link NLRP3 GST fusion protein pull-down Not specified 1-91 E.coli Not specified Full length In vitro translation 23066025
PYD ASC Link NLRP3 Two-hybrid Not specified 1-110 HEK293 Not specified 1-116 HEK293 11786556
PYD ASC Link NLRP3 Yeast two-hybrid Human Full length Yeast Human 1-101 Yeast 19337385
PYD ASC Link POP1 GST fusion protein pull-down Not specified 1-100 E.coli Human Not specified In vitro translation 12656673
PYD ASC Link POP1 GST fusion protein pull-down Not specified 1-91 E.coli Not specified 1-89 In vitro translation 23066025
PYD ASC Link POP1 In vitro purification protein assembly(Surface plasmon resonance) Not specified 1-96 E.coli Not specified 1-89 E.coli 18362139
PYD ASC Link POP2 GST fusion protein pull-down Human Not specified In vitro translation Not specified Human E.coli 17178784
PYD ASC Link POP2 GST fusion protein pull-down Not specified Human In vitro translation Human Not specified E.coli 17178784
PYD ASC Link POP2 Yeast two-hybrid Human Not specified Yeast Human Not specified Yeast 17178784
PYD ASC Link Pyrin His fusion protein pull-down Human Full length HEK293 Not specified Full length Sf-9 16037825
(Link: click this icon to show interactions only between the two corresponding DDs)
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In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
PYD ASC Link AIM2 Co-immunoprecipitation HEK293 Human Not specified Human 1-83 19158675
PYD ASC Link AIM2 Co-immunoprecipitation THP-1 19158676
PYD ASC Link AIM2 Co-immunoprecipitation HMVEC-d 21575908
PYD ASC Link AIM2 Co-immunoprecipitation NPC TW02 Human Full length Human Full length 22869553
PYD ASC Link AIM2 Fluorescence resonance energy transfer HEK293 Human Not specified Human Full length 19158675
PYD ASC Link AIM2 Fluorescence resonance energy transfer HEK293 Human Not specified Human 1-83 19158675
PYD ASC Link ASC Co-immunoprecipitation COS-7 Not specified 101-195 Not specified 101-195 11162571
PYD ASC Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 15030775
PYD ASC Link ASC Co-immunoprecipitation HEK293 Not specified 1-91 Not specified Full length 23066025
PYD ASC Link ASC Co-immunoprecipitation HEK293 Not specified 1-91 Not specified 1-96 23066025
PYD ASC Link IFI16 Co-Immunofluorescence staining HMVEC-d 21575908
PYD ASC Link IFI16 Co-immunoprecipitation HMVEC-d 21575908
PYD ASC Link NLRP1 Co-immunoprecipitation THP-1 17418785
PYD ASC Link NLRP1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 17418785
PYD ASC Link NLRP1 Co-immunoprecipitation Rat cortical lysate 19401709
PYD ASC Link NLRP1 Size-exclusion chromatography Mice cerebral cortex 19066616
PYD ASC Link NLRP10 Co-Immunofluorescence staining HEK293 20393137
PYD ASC Link NLRP10 Co-immunoprecipitation HEK293 Human Not specified Human Not specified 15096476
PYD ASC Link NLRP12 Co-Immunofluorescence staining HEK293 Not specified Not specified Not specified Full length 12019269
PYD ASC Link NLRP2 Co-immunoprecipitation HEK293 Not specified Not specified Human 1-93 15030775
PYD ASC Link NLRP2 Co-immunoprecipitation THP-1 Not used Human Full length 15030775
PYD ASC Link NLRP2 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 15456791
PYD ASC Link NLRP2 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Full length 15456791
PYD ASC Link NLRP3 Co-Immunofluorescence staining HEK293 Not specified Not specified Not specified Full length 11786556
PYD ASC Link NLRP3 Co-Immunofluorescence staining HEK293 Not specified Not specified Not specified 1-739 11786556
PYD ASC Link NLRP3 Co-Immunofluorescence staining HEK293 Human Full length Not specified Full length 16037825
PYD ASC Link NLRP3 Co-immunoprecipitation HEK293 Human Not specified Human 1-96 12615073
PYD ASC Link NLRP3 Co-immunoprecipitation HEK293 Not specified Not specified Not specified 1-112 15030775
PYD ASC Link NLRP3 Co-immunoprecipitation THP-1 15030775
PYD ASC Link NLRP3 Co-immunoprecipitation HEK293 Not specified Not specified Human 1-112 15030775
PYD ASC Link NLRP3 Co-immunoprecipitation HEK293 Human Not specified Not specified 1-87 19158675
PYD ASC Link NLRP3 Co-immunoprecipitation HMVEC-d 21575908
PYD ASC Link NLRP3 Co-immunoprecipitation HuH-7.5 21994322
PYD ASC Link NLRP3 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 22361007
PYD ASC Link NLRP3 Co-immunoprecipitation HEK293 Not specified 1-91 Not specified Full length 23066025
PYD ASC Link NLRP3 Fluorescence resonance energy transfer HEK293 Human Not specified Not specified 1-87 19158675
PYD ASC Link NLRP7 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 22361007
PYD ASC Link POP1 Co-Immunofluorescence staining COS-7 Not specified Not specified Not specified Not specified 12656673
PYD ASC Link POP1 Co-immunoprecipitation HEK293 Not specified 1-100 Human Not specified 12656673
PYD ASC Link POP1 Co-immunoprecipitation HEK293 Human Not specified Human Not specified 17178784
PYD ASC Link POP1 Co-immunoprecipitation HEK293 Not specified 1-91 Not specified 1-89 23066025
PYD ASC Link POP2 Co-Immunofluorescence staining HEK293 Human Not specified Human Not specified 17178784
PYD ASC Link POP2 Co-Immunofluorescence staining HeLa Not specified Not specified Human Not specified 17339483
PYD ASC Link POP2 Co-immunoprecipitation HEK293 Human Not specified Human Not specified 17178784
PYD ASC Link Pyrin Co-Immunofluorescence staining HeLa Not specified Not specified Not specified Not specified 11498534
PYD ASC Link Pyrin Co-Immunofluorescence staining PT67 Mouse Full length Mouse Full length 12667444
PYD ASC Link Pyrin Co-immunoprecipitation HEK293 Human Not specified Not specified Full length 12615073
PYD ASC Link Pyrin Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 16037825
PYD ASC Link Pyrin GST fusion protein pull-down PT67 Mouse Full length Mouse Not specified 12667444
(Link: click this icon to show interactions only between the two corresponding DDs)
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Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
PYD ASC Link ASC Co-immunoprecipitation E13A, K21A, R41A, D48A, D51A (ASC) 23066025
PYD ASC Link ASC GST fusion protein pull-down E13A, K21A, R41A, D48A, D51A (ASC) 23066025
PYD ASC Link NLRP2 Co-immunoprecipitation E13W (ASC) 15456791
PYD ASC Link NLRP3 Co-immunoprecipitation E13A, K21A, R41A, D48A, D51A (ASC) 23066025
PYD ASC Link NLRP3 GST fusion protein pull-down E13A, K21A, R41A, D48A, D51A (ASC) 23066025
PYD ASC Link POP1 Co-immunoprecipitation E13A, K21A, R41A, D48A, D51A (ASC) 23066025
PYD ASC Link POP1 GST fusion protein pull-down R3A, D10A, E13A, K21A, R38A, R41A, D48A, D51A, D54A, Q84A (ASC) 23066025
PYD ASC Link POP1 Surface plasmon resonance 1:1 4.8μM D6A and L25A, E13A and L25A, K21A, Y36A, D48A and L25A, D54A and L25A (ASC) 18362139
(Link: click this icon to show interactions only between the two corresponding DDs)
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Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
PYD ASC Link AIM2 Luciferase assay NF-kB activation (Inflammasome) 19158675
PYD ASC Link AIM2 Caspase cleaved assay Caspase1 activation (Inflammasome) 19158676
PYD ASC Link NLRP10 Propidium iodide-Annexin V staining, Luciferase assay, Immunostaining Apoptosis inhibition NF-kB-activation block, ASC aggregation inhibition 20393137
PYD ASC Link NLRP10 Annexin V staining, EMSA ASC mediated Apoptosis block NF-kB-activation block 15096476
PYD ASC Link NLRP12 Luciferase assay, ELISA NF-kB activation, IL-1b secretion 12019269
PYD ASC Link NLRP2 ELISA IL-1b secretion (Inflammasome) 15456791
PYD ASC Link NLRP3 Luciferase assay NF-kB activation (Inflammasome) 11786556
PYD ASC Link NLRP3 Caspase cleaved assay Caspase1 activation (Inflammasome) 16037825
PYD ASC Link NLRP3 Luciferase assay NF-kB activation (Inflammasome) 12615073
PYD ASC Link NLRP3 Caspase cleaved assay Caspase1 activation (Inflammasome) 15030775
PYD ASC Link POP1 Luciferase assay NF-kB activation block 12656673
PYD ASC Link POP2 ELISA IL-1b secretion decrease 17178784
PYD ASC Link POP2 Immunofluorescence staining Inflammasome inhibition 17339483
PYD ASC Link Pyrin DAPI staining Apoptosis inhibition 11498534
PYD ASC Link Pyrin Apoptotic DNA ladder assay Apoptosis inhibition 12667444
PYD ASC Link Pyrin β-galactosidase staining, Luciferase assay Apoptosis inhibition NLRP3-ASC mediated NF-kB activation block 12615073
PYD ASC Link Pyrin Caspase cleaved assay Caspase1 activation (Inflammasome) 16037825
(Link: click this icon to show interactions only between the two corresponding DDs)