Apaf1 (CARD)

Full Name:
Apoptotic protease-activating factor 1
Alternative Names:
CED4, APAF-1, DKFZp781B1145
Function:
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Death Domain Subfamily & Boundary Region:
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq O14727external db link DIP-27624Nexternal db link O14727external db link hsa:317external db link O14727external db link MINT-96751external db link
Mouse Seq O88879external db link O88879external db link O88879external db link
Rat Seq Q9EPV5external db link Q9EPV5external db link rno:78963external db link MINT-3370020external db link
Dog Seq Q9GL23external db link Q9GL23external db link
Zebra fish Seq Q9I9H8external db link Q9I9H8external db link dre:58131external db link
Structure Species Residue Method Expression Purification Column Final condition Final concentration Reference
1C15 Download Human 1-97 NMR E.coli Gel filtration Sephadex G-50 50 mM NaN3 10500165
1CWW Download Human 1-97 NMR E.coli Gel filtration Superdex 75 20mM sodium phosphate(pH6.7), 75mM NaCl, 2mM DTT, 0.04% sodium azide 1.5mM 10578182
1CY5 Download Human 1-97 X-ray Ion Exchange 10543941
1Z6T Download Human 1-591 X-ray E.coli Ion Exchange source15Q 20mM HEPES(pH7.8), 250mM NaCl, 2mM DTT 3mg/ml 15829969
2P1H Download Human 1-92 X-ray E.coli 10mM Tris(pH7.5), 10mM NaCl 5mg/ml 17408690
2YGS Download Human 1-92 X-ray E.coli Gel filtration Superdex 75 10376594
3IZA Download Human 1-1248 Electron microscopy 20462491
3YGS Download Human 1-95 X-ray E.coli Gel filtration Superdex 75 20mg/ml 10376594
3SFZ Download Mouse 1-1249 X-ray Sf21 Gel filtration Superdex 200 50mM HEPES(pH7.5, 100mM NaCl, 2mM DTT, 1mM MgCl2 15mg/ml 21827944
No information
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
CARD Apaf1 Link Apaf1 In vitro purification protein assembly(Size-exclusion chromatography) Not specified Not specified Not specified Not specified Not specified Not specified 10944114
CARD Apaf1 Link Caspase9 GST fusion protein pull-down Not specified 1-97 E.coli Not specified Not specified In vitro translation 9390557
CARD Apaf1 Link Caspase9 GST fusion protein pull-down Not specified 1-97 E.coli Not specified Not specified In vitro translation 9922454
CARD Apaf1 Link Caspase9 GST fusion protein pull-down Not specified 1-420 In vitro translation Not specified Not specified E.coli 11408476
CARD Apaf1 Link Caspase9 GST fusion protein pull-down Not specified Not specified E.coli Not specified Not specified In vitro translation 11821383
CARD Apaf1 Link Caspase9 GST fusion protein pull-down Human THP-1 lysate Not specified 1-130 E.coli 14993223
CARD Apaf1 Link Caspase9 GST fusion protein pull-down Not specified Not specified E.coli Not specified D32cell lysate 15542838
CARD Apaf1 Link Caspase9 GST fusion protein pull-down Not specified D32cell lysate Not specified Not specified E.coli 15542838
CARD Apaf1 Link Caspase9 In vitro purification protein assembly(Size-exclusion chromatography) Human Full length HeLa Not specified Full length HeLa 10206961
CARD Apaf1 Link Caspase9 In vitro purification protein assembly(Size-exclusion chromatography) Human 1-97 E.coli Human 1-112 E.coli 10376594
CARD Apaf1 Link Caspase9 In vitro purification protein assembly(Size-exclusion chromatography) Not specified THP-1 lysate Not specified THP-1 lysate 11230124
CARD Apaf1 Link Caspase9 In vitro purification protein assembly(Size-exclusion chromatography) Not specified 1-97 E.coli Not specified Full length E.coli 11904389
CARD Apaf1 Link Caspase9 In vitro purification protein assembly(Size-exclusion chromatography) Not specified 1-97 E.coli Not specified 1-132 E.coli 11904389
CARD Apaf1 Link Caspase9 In vitro purification protein assembly(Size-exclusion chromatography) Not specified Not specified E.coli Not specified Not specified E.coli 19494828
CARD Apaf1 Link Caspase9 Yeast two-hybrid Human 1-92 Yeast Human 1-97 Yeast 19337385
CARD Apaf1 Link NLRP1 GST fusion protein pull-down Not specified Not specified In vitro translation Not specified Not specified E.coli 11113115
(Link: click this icon to show interactions only between the two corresponding DDs)
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In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD Apaf1 Link Apaf1 Co-immunoprecipitation HEK293 Not specified Full length Not specified 1-559 9837928
CARD Apaf1 Link Apaf1 Co-immunoprecipitation HEK293 Not specified 1-559 Not specified 1-559 9837928
CARD Apaf1 Link Apaf1 Co-immunoprecipitation HEK293 Not specified 1-559 Not specified 1-455 9837928
CARD Apaf1 Link Apaf1 Co-immunoprecipitation HEK293 Human Not specified Human Not specified 10944114
CARD Apaf1 Link Caspase2 Size-exclusion chromatography HeLa 12460989
CARD Apaf1 Link Caspase4 Co-immunoprecipitation HEK293 Not specified Full length Human Not specified 9539746
CARD Apaf1 Link Caspase9 Co-immunoprecipitation HeLa S-100 9390557
CARD Apaf1 Link Caspase9 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 9488720
CARD Apaf1 Link Caspase9 Co-immunoprecipitation HEK293 Not specified 1-412 Not specified Full length 9488720
CARD Apaf1 Link Caspase9 Co-immunoprecipitation HEK293 Not specified 1-412 Not specified 1-168 9488720
CARD Apaf1 Link Caspase9 Co-immunoprecipitation HEK293 Not specified Full length Human Not specified 9539746
CARD Apaf1 Link Caspase9 Co-immunoprecipitation HEK293 Not specified 1-559 Human Not specified 9539746
CARD Apaf1 Link Caspase9 Co-immunoprecipitation Rat cardiomyocyte 15253891
CARD Apaf1 Link Caspase9 Size-exclusion chromatography HEK293 10617566
CARD Apaf1 Link Caspase9 Size-exclusion chromatography Human monocytic tumor cell 10692394
CARD Apaf1 Link Caspase9 Size-exclusion chromatography THP-1 11550094
CARD Apaf1 Link Caspase9 Size-exclusion chromatography HeLa 12460989
CARD Apaf1 Link Caspase9 Size-exclusion chromatography Rat cortex 14602082
CARD Apaf1 Link NLRP1 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 11113115
CARD Apaf1 Link NLRP1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 17418785
(Link: click this icon to show interactions only between the two corresponding DDs)
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Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
CARD Apaf1 Link Caspase9 Structure 1:1 D27A, E40A (Apaf1) R13A, R56A (Caspase9) 3YGS 10376594
CARD Apaf1 Link Caspase9 Gel filtration 1:1 11904389
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
CARD Apaf1 Link Apaf1 Caspase cleaved assay Caspase9 activation 9837928
CARD Apaf1 Link Apaf1 Caspase cleaved assay Caspase9 activation 10944114
CARD Apaf1 Link Caspase9 β-galactosidase staining Apoptosis (Apoptosome) 9390557
CARD Apaf1 Link Caspase9 DAPI staining Apoptosis (Apoptosome) 9488720
CARD Apaf1 Link Caspase9 β-galactosidase staining Apoptosis (Apoptosome) 9539746
CARD Apaf1 Link Caspase9 DAPI staining Apoptosis (Apoptosome) 11408476
CARD Apaf1 Link Caspase9 Caspase cleaved assay Caspase9 activation 10206961
CARD Apaf1 Link Caspase9 Caspase cleaved assay Caspase9 activation (Apoptosome) 11904389
CARD Apaf1 Link Caspase9 Caspase cleaved assay Caspase9 activation 10617566
CARD Apaf1 Link NLRP1 DAPI staining Apoptosis 11113115
(Link: click this icon to show interactions only between the two corresponding DDs)