CARD16 (CARD)

Full Name:
Caspase recruitment domain-containing protein 16
Alternative Names:
COP, COP1, PSEUDO-ICE
Function:
Caspase inhibitor. Acts as a regulator of procaspase-1/CASP1 activation implicated in the regulation of the proteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release of IL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro-inflammatory cytokine response. Also able to inhibit CASP1-mediated neuronal cell death, TNF-alpha, hypoxia-, UV-, and staurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspases CASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2.
Death Domain Subfamily & Boundary Region:
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq Q5EG05external db link Q5EG05external db link hsa:114769external db link
Not solved yet
No information
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
CARD CARD16 Link ASC GST fusion protein pull-down Human Full length E.coli Human 105-195 In vitro translation 11967258
CARD CARD16 Link ASC GST fusion protein pull-down Human Full length E.coli Human Full length In vitro translation 11967258
CARD CARD16 Link CARD18 GST fusion protein pull-down Human 1-106 E.coli Human Full length In vitro translation 11536016
CARD CARD16 Link CARD8 GST fusion protein pull-down Not specified Not specified In vitro translation Not specified Full length E.coli 11821383
CARD CARD16 Link Caspase1 GST fusion protein pull-down Human 1-106 E.coli Not specified Not specified In vitro translation 11536016
CARD CARD16 Link Caspase1 GST fusion protein pull-down Not specified Not specified E.coli Not specified Not specified In vitro translation 11821383
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD CARD16 Link CARD16 Co-immunoprecipitation HEK293 Human Not specified Human Not specified 11432859
CARD CARD16 Link CARD17 Co-immunoprecipitation HEK293 Human 1-106 Human Not specified 15383541
CARD CARD16 Link CARD18 Co-immunoprecipitation HEK293 Human 1-106 Human Full length 11536016
CARD CARD16 Link CARD8 Co-immunoprecipitation HEK293 Not specified Full length Not specified Not specified 11821383
CARD CARD16 Link CARD8 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Full length 11821383
CARD CARD16 Link Caspase1 Co-immunoprecipitation HEK293 Human Not specified Not specified Full length 11432859
CARD CARD16 Link Caspase1 Co-immunoprecipitation HEK293 Human Not specified Not specified 1-120 11432859
CARD CARD16 Link Caspase1 Co-immunoprecipitation HEK293 Human 1-106 Not specified Not specified 11536016
CARD CARD16 Link RIPK2 Co-immunoprecipitation HEK293 Human Not specified Not specified Full length 11432859
CARD CARD16 Link RIPK2 Co-immunoprecipitation HEK293 Human 1-106 Not specified Not specified 11536016
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
CARD CARD16 Link Caspase1 Immunoprecipitation, ELISA Rip mediated procaspase1 oligomerization inhibition, IL-1b secretion decrease 11432859
CARD CARD16 Link Caspase1 Immunoprecipitation Caspase1-RIPK2 complex interfere 11536016
CARD CARD16 Link RIPK2 Immunoprecipitation Caspase1-RIPK2 complex interfere 11536016
(Link: click this icon to show interactions only between the two corresponding DDs)