Caspase1 (CARD)

Full Name:
Caspase-1
Alternative Names:
ICE, P45, IL1BC, CASP1
Function:
Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
Death Domain Subfamily & Boundary Region:
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq P29466external db link DIP-175Nexternal db link P29466external db link hsa:834external db link P29466external db link MINT-201196external db link
Mouse Seq P29452external db link DIP-34659Nexternal db link P29452external db link mmu:12362external db link P29452external db link
Rat Seq P43527external db link DIP-29840Nexternal db link P43527external db link
Dog Seq Q9MZV7external db link Q9MZV7external db link cfa:403724external db link
Not solved yet
No information
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
CARD Caspase1 Link ASC GST fusion protein pull-down Not specified Not specified E.coli Human 105-195 In vitro translation 11967258
CARD Caspase1 Link ASC GST fusion protein pull-down Not specified Not specified E.coli Human Full length In vitro translation 11967258
CARD Caspase1 Link ASC GST fusion protein pull-down Not specified Not specified In vitro translation Not specified Not specified E.coli 14634131
CARD Caspase1 Link ASC Yeast two-hybrid Human 1-91 Yeast Human 105-195 Yeast 19337385
CARD Caspase1 Link CARD12 GST fusion protein pull-down Not specified Full length Not specified Not specified 1-88 Not specified 11390368
CARD Caspase1 Link CARD16 GST fusion protein pull-down Not specified Not specified In vitro translation Human 1-106 E.coli 11536016
CARD Caspase1 Link CARD16 GST fusion protein pull-down Not specified Not specified In vitro translation Not specified Not specified E.coli 11821383
CARD Caspase1 Link CARD18 GST fusion protein pull-down Not specified Not specified In vitro translation Human Full length E.coli 11536016
CARD Caspase1 Link CARD18 In vitro purification protein assembly(immunoprecipitation) Not specified 1-92 E.coli Not specified Full length E.coli 11051551
CARD Caspase1 Link CARD8 GST fusion protein pull-down Not specified Not specified In vitro translation Not specified Full length E.coli 11821383
CARD Caspase1 Link CARD8 Yeast two-hybrid Human 1-91 Yeast Human 342-430 Yeast 19337385
CARD Caspase1 Link RIPK2 GST fusion protein pull-down Not specified 1-92 In vitro translation Not specified 454-540 E.coli 11051551
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD Caspase1 Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Human 105-195 11967258
CARD Caspase1 Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 11967258
CARD Caspase1 Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Not specified 92-195 12019269
CARD caspase1 Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 12191486
CARD Caspase1 Link ASC Co-immunoprecipitation PT67 Mouse Not specified Mouse Full length 12667444
CARD Caspase1 Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 14634131
CARD Caspase1 Link ASC Co-immunoprecipitation THP-1 14634131
CARD Caspase1 Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 16585594
CARD Caspase1 Link ASC Co-immunoprecipitation THP-1 16585594
CARD Caspase1 Link ASC Co-immunoprecipitation Rat cortical lysate 19401709
CARD Caspase1 Link ASC Co-immunoprecipitation HEK239 Human Not specified Human Not specified 21862576
CARD Caspase1 Link ASC GST fusion protein pull-down PT67 Mouse Not specified Mouse Full length 12667444
CARD Caspase1 Link ASC Size-exclusion chromatography Mice cerebral cortex 19066616
CARD Caspase1 Link CARD12 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 11374873
CARD Caspase1 Link CARD12 Co-immunoprecipitation HEK293 Human Full length Not specified Full length 11390368
CARD Caspase1 Link CARD12 Co-immunoprecipitation HEK293 Human 1-94 Not specified 1-88 11390368
CARD Caspase1 Link CARD12 Co-immunoprecipitation HEK293 Not specified Not specified Human Not specified 11472070
CARD Caspase1 Link CARD12 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 17418785
CARD Caspase1 Link CARD16 Co-immunoprecipitation HEK293 Not specified Full length Human Not specified 11432859
CARD Caspase1 Link CARD16 Co-immunoprecipitation HEK293 Not specified 1-120 Human Not specified 11432859
CARD Caspase1 Link CARD16 Co-immunoprecipitation HEK293 Not specified Not specified Human 1-106 11536016
CARD Caspase1 Link CARD17 Co-immunoprecipitation HEK293 Not specified Not specified Human Not specified 15383541
CARD Caspase1 Link CARD18 Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 11536016
CARD Caspase1 Link CARD8 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Full length 11821383
CARD Caspase1 Link CARD8 Co-immunoprecipitation HEK293 Not specified Full length Not specified Not specified 11821383
CARD Caspase1 Link CARD8 Co-immunoprecipitation HEK293 Not specified Not specified Not specified 320-431 15030775
CARD Caspase1 Link Caspase1 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 11432859
CARD Caspase1 Link Caspase1 Co-immunoprecipitation HEK239 Human Not specified Human Not specified 21862576
CARD Caspase1 Link Caspase12 Co-immunoprecipitation HEK293 Not specified Not specified Rodent Not specified 16625199
CARD Caspase1 Link Caspase12 Co-immunoprecipitation HEK293 Not specified Not specified Rat Full length 18332441
CARD Caspase1 Link NLRP1 Co-immunoprecipitation HEK293 Human Not specified Human Full length 17349957
CARD Caspase1 Link NLRP1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 17418785
CARD Caspase1 Link NLRP1 Co-immunoprecipitation Rat cortical lysate 19401709
CARD Caspase1 Link NLRP1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 21252346
CARD Caspase1 Link NLRP1 Size-exclusion chromatography Mice cerebral cortex 19066616
CARD Caspase1 Link NOD1 Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 10329646
CARD Caspase1 Link NOD1 Co-immunoprecipitation HEK293 Not specified 1-102 Not specified 1-158 12459189
CARD Caspase1 Link NOD2 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 18511561
CARD Caspase1 Link RIPK2 Co-immunoprecipitation HEK293 Human 2-540 Human 2-92 9705938
CARD Caspase1 Link RIPK2 Co-immunoprecipitation HEK293 Not specified Full length Not specified Not specified 11051551
CARD Caspase1 Link RIPK2 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 11536016
CARD Caspase1 Link RIPK2 Co-immunoprecipitation HEK293 Human 2-540 Murine Not specified 15039421
CARD Caspase1 Link RIPK2 Co-immunoprecipitation HEK293 Human 431-540 Murine Not specified 15039421
CARD Caspase1 Link RIPK2 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 16585594
CARD Caspase1 Link RIPK2 Co-immunoprecipitation THP-1 16585594
CARD Caspase1 Link RIPK2 Co-immunoprecipitation HEK239 Human Not specified Not specified Not specified 21862576
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
CARD Caspase1 Link ASC Co-immunoprecipitation L122Q(ASC) 14634131
CARD Caspase1 Link ASC Co-immunoprecipitation D27G, R45D (Caspase1) 21862576
CARD Caspase1 Link Caspase1 Co-immunoprecipitation D27G, R45D (Caspase1) 21862576
CARD Caspase1 Link RIPK2 Co-immunoprecipitation D27G, R45D (Caspase1) 21862576
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
CARD Caspase1 Link ASC ELISA IL-1 secretion (Inflammasome) 11967258
CARD Caspase1 Link ASC ELISA IL-1b secretion (Inflammasome) 12019269
CARD Caspase1 Link ASC ELISA IL-1b secretion decrease 14634131
CARD Caspase1 Link ASC ELISA IL-1b secretion 16585594
CARD Caspase1 Link ASC Luciferase assay NF-kB activation 21862576
CARD Caspase1 Link CARD12 β-galactosidase staining Pyroptosis (Inflammasome) 11390368
CARD Caspase1 Link CARD16 Immunoprecipitation, ELISA Rip mediated procaspase1 oligomerization inhibition, IL-1b secretion decrease 11432859
CARD Caspase1 Link CARD16 Immunoprecipitation Caspase1-RIPK2 complex interfere 11536016
CARD Caspase1 Link CARD18 Immunoprecipitation Caspase1-RIPK2 complex interfere 11536016
CARD Caspase1 Link CARD18 Caspase1 binding assay RIPK2 ability inhibition 11051551
CARD Caspase1 Link CARD8 Caspase cleaved assay, ELISA Caspase1 activation block, IL-1b secretion decrease 11821383
CARD Caspase1 Link Caspase12 Caspase cleaved assay Caspase12 activation 18332441
CARD Caspase1 Link NLRP1 Luciferase assay Caspase1 activation (Inflammasome) 17349957
CARD Caspase1 Link NLRP1 ELISA IL-1b secretion increase 17418785
CARD Caspase1 Link NLRP1 Caspase cleaved assay Caspase1 activation (Inflammasome) 21252346
CARD Caspase1 Link NOD1 Immunoprecipitation Caspase1 oligomerization enhance 12459189
CARD Caspase1 Link NOD2 Caspase cleaved assay Caspase1 activation 18511561
CARD Caspase1 Link RIPK2 Caspase cleaved assay Pro-caspase1 activation 9705938
CARD Caspase1 Link RIPK2 Caspase cleaved assay Caspase1 activation 11051551
CARD Caspase1 Link RIPK2 Reporter gene assay NF-kB activation 15039421
CARD Caspase1 Link RIPK2 Luciferase assay NF-kB activation 16585594
(Link: click this icon to show interactions only between the two corresponding DDs)