DR4 (DD)

Full Name:
Tumor necrosis factor receptor superfamily member 10A
Alternative Names:
APO2, CD261, MGC9365, TRAILR1, TRAILR-1, TNFRSF10A
Function:
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.
Death Domain Subfamily & Boundary Region:
  • DD : 365-448 Seq
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq O00220external db link DIP-33565Nexternal db link O00220external db link hsa:8797external db link O00220external db link MINT-99073external db link
Not solved yet
No information
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD DR4 Link DR5 Affinity purification-mass spectrometry H460cell lyaste H460cell lyaste 19427028
DD DR4 Link FADD Affinity purification-mass spectrometry H460cell lyaste H460cell lyaste 19427028
DD DR4 Link FADD GST fusion protein pull-down Not specified 269-468 E.coli HeLa lysate 12920112
DD DR4 Link FADD Yeast two-hybrid Not specified 272-469 Yeast Not specified Full length Yeast 15452120
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD DR4 Link DR5 Co-immunoprecipitation HeLa 16980609
DD DR4 Link FADD Co-immunoprecipitation HEK293 Human 106-468 Human Not specified 9430227
DD DR4 Link FADD Co-immunoprecipitation HEK293 Not specified 261-465 Not specified 80-208 9430228
DD DR4 Link FADD Co-immunoprecipitation BAJB Not specified 272-469 Not specified Full length 15452120
DD DR4 Link FADD Co-immunoprecipitation HeLa 16980609
DD DR4 Link FADD Co-immunoprecipitation SW948 21272366
DD DR4 Link MADD Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 15208670
DD DR4 Link MADD Co-immunoprecipitation HEK293 Not specified Not specified Human 628-1376 15208670
DD DR4 Link MADD Fluorescence resonance energy transfer HeLa Not specified Not specified Human Full length 15208670
DD DR4 Link RIP Co-immunoprecipitation HEK293 Human 1-351 Human 559-671 10958661
DD DR4 Link RIP His fusion protein pull-down HEK293 Human 106-468 Human Not specified 9430227
DD DR4 Link TRADD Co-immunoprecipitation HEK293 Human 106-468 Human 121-312 9430227
DD DR4 Link TRADD Co-immunoprecipitation HEK293 Not specified 261-465 Not specified Not specified 9430228
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DD DR4 Link FADD β-galactosidase staining Apoptosis (DISC) 9430227
DD DR4 Link FADD Cytoplasmic histone associated DNA fragment detection Apoptosis (DISC) 9430228
DD DR4 Link FADD Caspase cleaved assay Caspase8 activation 15452120
DD DR4 Link FADD Immunofluorescence staining Apoptosis (DISC) 21272366
DD DR4 Link FADD Annexin V staining Apoptosis (DISC) 12920112
DD DR4 Link MADD Immunofluorescence staining Apoptosis (DISC) 15208670
DD DR4 Link RIP IKK assay, JNK assay IkB activation, JNK activation 10958661
DD DR4 Link TRADD Luciferase assay NF-kB activation 9430227
(Link: click this icon to show interactions only between the two corresponding DDs)