FADD (DD)

Full Name:
Protein FADD
Alternative Names:
GIG3, MORT1, MGC8528
Function:
Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
Death Domain Subfamily & Boundary Region:
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq Q13158external db link DIP-286Nexternal db link Q13158external db link hsa:8772external db link Q13158external db link MINT-91814external db link
Mouse Seq Q61160external db link DIP-34771Nexternal db link Q61160external db link mmu:14082external db link Q61160external db link MINT-1514991external db link
Rat Seq Q8R2E7external db link Q8R2E7external db link rno:266610external db link
Structure Species Residue Method Expression Purification Column Final condition Final concentration Reference
1E41 Download Human 93-192 NMR E.coli Gel filtration Superose 12 50mM potassium phosphate(pH6.2), 150mM NaCl, 1mM DTT 2mM 10964568
2GF5 Download Human 2-191 NMR E.coli Affinity chromatography 50mM potassium phosphate(pH4.0), 150mM NaCl, 1mM DTT 1.5mM 16762833
3EZQ Download Human 93-208 X-ray E.coli Ion Exchange SourceQ 10/10 10mM Hepes(pH7.8), 300mM NaCl, 2mM DTT 10mg/ml 19118384
3OQ9 Download Human 93-184 X-ray E.coli Gel filtration Superdex 200 20935634
1FAD Download Mouse 89-183 NMR E.coli Gel filtration 10mg/ml 10347191
Disease Mutation in disease Reference
Infections, recurrent with encephalopathy, hepatic dysfunction and cardiovasuclar malforamtions C105W 21109225
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD FADD Link ANK3 GST fusion protein pull-down Not specified Not specified Not specified Rat Not specified Not specified 14694156
DD FADD Link DR4 Affinity purification-mass spectrometry H460cell lyaste H460cell lyaste 19427028
DD FADD Link DR4 GST fusion protein pull-down HeLa lysate Not specified 269-468 E.coli 12920112
DD FADD Link DR4 Yeast two-hybrid Not specified Full length Yeast Not specified 272-469 Yeast 15452120
DD FADD Link DR5 GST fusion protein pull-down Jurkat lysate Not specified 191-411 E.coli 12721308
DD FADD Link DR5 GST fusion protein pull-down HeLa lysate Not specified 209-411 E.coli 12920112
DD FADD Link DR5 Yeast two-hybrid Not specified Full length Yeast Not specified 209-412 Yeast 15173180
DD FADD Link DR5 Yeast two-hybrid Not specified Full length Yeast Not specified 209-412 Yeast 15452120
DD FADD Link Fas Affinity purification-mass spectrometry Human 93-184 E.coli Mouse 210-310 E.coli 20935634
DD FADD Link Fas Affinity purification-mass spectrometry Human 93-191 E.coli Human 218-318 E.coli 20935634
DD FADD Link Fas GST fusion protein pull-down Not specified Not specified Not specified Human 175-319 Not specified 7536190
DD FADD Link Fas GST fusion protein pull-down Not specified Not specified Yeast Not specified Not specified Yeast 7538907
DD FADD Link Fas GST fusion protein pull-down Not specified Not specified In vitro translation Not specified Not specified E.coli 8875942
DD FADD Link Fas GST fusion protein pull-down Human 80-205 In vitro translation Murine 165-295 Not specified 9990010
DD FADD Link Fas GST fusion protein pull-down Not specified Not specified In vitro translation Human 174-319 E.coli 10200300
DD FADD Link Fas GST fusion protein pull-down Jurkat lysate Not specified 191-335 E.coli 12721308
DD FADD Link Fas GST fusion protein pull-down Human Full length In vitro translation Human Not specified Not specified 14573612
DD FADD Link Fas His fusion protein pull-down Human 93-208 E.coli Human 223-335 E.coli 19118384
DD FADD Link Fas His fusion protein pull-down Not specified Not specified E.coli Not specified Not specified E.coli 20935634
DD FADD Link Fas In vitro purification protein assembly(Pull down) Not specified Not specified HEK293 Not specified 1-304 E.coli 7538907
DD FADD Link Fas In vitro purification protein assembly(Size-exclusion chromatography) Human 93-208 E.coli Human 223-335 E.coli 19118384
DD FADD Link Fas In vitro purification protein assembly(Size-exclusion chromatography) Human 93-184 E.coli Human 210-310 E.coli 20935634
DD FADD Link Fas In vitro purification protein assembly(Surface plasmon resonance) Not specified 89-183 E.coli Human Not specified E.coli 10347191
DD FADD Link Fas Yeast two-hybrid Not specified 130-245 Yeast Human 175-319 Yeast 7536190
DD FADD Link Fas Yeast two-hybrid Not specified 130-245 Yeast Human 200-319 Yeast 7536190
DD FADD Link Fas Yeast two-hybrid Not specified Not specified In vitro translation Not specified 1-304 E.coli 7538907
DD FADD Link Fas Yeast two-hybrid Not specified 92-208 Yeast Not specified 210-319 Yeast 8642271
DD FADD Link Fas Yeast two-hybrid Human 80-208 Yeast Human 177-335 Yeast 11464214
DD FADD Link Fas Yeast two-hybrid Not specified Full length Yeast Human 191-313 Yeast 12107169
DD FADD Link Fas Yeast two-hybrid Not specified Not specified Yeast Not specified 177-335 Yeast 15173180
DD FADD Link RIP Yeast two-hybrid Not specified 92-208 Yeast Not specified 261-372 Yeast 8642271
DD FADD Link RIP Yeast two-hybrid Human Full length Yeast Human 580-671 Yeast 10755617
DD FADD Link RIP Yeast two-hybrid Human 80-208 Yeast Human Full length Yeast 11464214
DD FADD Link RIP1 GST fusion protein pull-down Human 93-184 E.coli Not specified 583-663 E.coli 22922561
DD FADD Link RIP1 In vitro purification protein assembly(Size-exclusion chromatography) Human 93-184 E.coli Not specified 583-663 E.coli 22922561
DD FADD Link RIP1 In vitro purification protein assembly(Surface plasmon resonance) Human 93-184 E.coli Not specified 583-663 E.coli 22922561
DD FADD Link TNFR1 Yeast two-hybrid Not specified 92-208 Yeast Not specified 326-426 Yeast 8642271
DD FADD Link TRADD GST fusion protein pull-down Not specified Full length E.coli Not specified 243-455 In vitro translation 16006552
DD FADD Link TRADD Yeast two-hybrid Not specified Full length Yeast Not specified Full length Yeast 8565075
DD FADD Link TRADD Yeast two-hybrid Not specified Full length Yeast Not specified 55-312 Yeast 8565075
DD FADD Link TRADD Yeast two-hybrid Not specified Full length Yeast Not specified 195-312 Yeast 8565075
DD FADD Link TRADD Yeast two-hybrid Not specified 92-208 Yeast Not specified 195-312 Yeast 8642271
DD FADD Link TRADD Yeast two-hybrid Human 80-208 Yeast Human Full length Yeast 11464214
DD FADD Link TRADD Yeast two-hybrid Not specified Full length Yeast Not specified Full length Yeast 12107169
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD FADD Link ANK3 Co-Immunofluorescence staining MDCK Not specified Not specified Rat Not specified 14694156
DD FADD Link ANK3 Co-immunoprecipitation MDCK Not specified Not specified Rat Not specified 14694156
DD FADD Link DAPK1 Co-Immunofluorescence staining Rat brain cell 12911633
DD FADD Link DAPK1 Co-immunoprecipitation Rat brain cell 12911633
DD FADD Link DAPK1 Co-immunoprecipitation Human Epilepsy brain lysate 15048887
DD FADD Link DAPK1 Co-immunoprecipitation MBA-MB-231 21168190
DD FADD Link DR3 Co-immunoprecipitation HEK293 Not specified Not specified Human 332-413 9052839
DD FADD Link DR3 Co-immunoprecipitation HEK293 Human Not specified Human 27-418 9430227
DD FADD Link DR4 Co-immunoprecipitation HEK293 Human Not specified Human 106-468 9430227
DD FADD Link DR4 Co-immunoprecipitation HEK293 Not specified 80-208 Not specified 261-465 9430228
DD FADD Link DR4 Co-immunoprecipitation BAJB Not specified Full length Not specified 272-469 15452120
DD FADD Link DR4 Co-immunoprecipitation HeLa 16980609
DD FADD Link DR4 Co-immunoprecipitation SW948 21272366
DD FADD Link DR5 Co-immunoprecipitation HEK293 Human Not specified Human 51-411 9430227
DD FADD Link DR5 Co-immunoprecipitation HEK293 Not specified 80-208 Not specified 232-439 9430228
DD FADD Link DR5 Co-immunoprecipitation BJAB 15173180
DD FADD Link DR5 Co-immunoprecipitation HeLa Not specified 272-469 Not specified 79-208 15173180
DD FADD Link DR5 Co-immunoprecipitation BJAB Not specified Full length Not specified 209-412 15452120
DD FADD Link DR5 Co-immunoprecipitation HepG2 16980609
DD FADD Link DR5 Co-immunoprecipitation HeLa 17585340
DD FADD Link DR5 Co-immunoprecipitation SW948 21272366
DD FADD Link Fas Jurkat 21557211
DD FADD Link Fas Co-Immunofluorescence staining Jurkat 12702723
DD FADD Link Fas Co-Immunofluorescence staining BJAB Not specified Not specified Not specified Not specified 16498403
DD FADD Link Fas Co-immunoprecipitation HeLa Not specified Not specified Not specified Not specified 7536190
DD FADD Link Fas Co-immunoprecipitation HEK293 Not specified Not specified Not specified 1-304 7538907
DD FADD Link Fas Co-immunoprecipitation HEK293 Not specified 80-208 Not specified Not specified 8617770
DD FADD Link Fas Co-immunoprecipitation HEK293 Not specified 2-208 Not specified Not specified 8617770
DD FADD Link Fas Co-immunoprecipitation A20 Murine B cell 8649383
DD FADD Link Fas Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 9082980
DD FADD Link Fas Co-immunoprecipitation BJAB 9325306
DD FADD Link Fas Co-immunoprecipitation HEK293 Human Not specified Human 19-335 9430227
DD FADD Link Fas Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 9714541
DD FADD Link Fas Co-immunoprecipitation DT-40 chicken lymphoma B cell 9880544
DD FADD Link Fas Co-immunoprecipitation HE29 10075697
DD FADD Link Fas Co-immunoprecipitation HEK293 Not used Human 174-319 10200300
DD FADD Link Fas Co-immunoprecipitation A20 Murine B cell 10452970
DD FADD Link Fas Co-immunoprecipitation H9 10607716
DD FADD Link Fas Co-immunoprecipitation SHEP neuroblastoma cell 10919673
DD FADD Link Fas Co-immunoprecipitation CD40L-induced B blast 10975811
DD FADD Link Fas Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 11032752
DD FADD Link Fas Co-immunoprecipitation Tonsillar germinal center B cell 11181697
DD FADD Link Fas Co-immunoprecipitation HT29 11313933
DD FADD Link Fas Co-immunoprecipitation Jurkat 11729181
DD FADD Link Fas Co-immunoprecipitation SKW6.4 11729181
DD FADD Link Fas Co-immunoprecipitation SKW6.4 11739735
DD FADD Link Fas Co-immunoprecipitation H9 11739735
DD FADD Link Fas Co-immunoprecipitation Mouse brain cell 11978827
DD FADD Link Fas Co-immunoprecipitation SKW6.4 12110583
DD FADD Link Fas Co-immunoprecipitation H9 12110583
DD FADD Link Fas Co-immunoprecipitation Human Jurkat cell 12878044
DD FADD Link Fas Co-immunoprecipitation HuT-78 12878044
DD FADD Link Fas Co-immunoprecipitation MCF-7 Human Full length Not used 14573612
DD FADD Link Fas Co-immunoprecipitation HEK293 15383280
DD FADD Link Fas Co-immunoprecipitation Human neuronal progenitor cell 15545353
DD FADD Link Fas Co-immunoprecipitation SKW6.4 16498403
DD FADD Link Fas Co-immunoprecipitation SKW6.4 17159907
DD FADD Link Fas Co-immunoprecipitation T98G 18328427
DD FADD Link Fas Co-immunoprecipitation LN-18 18328427
DD FADD Link Fas Fluorescence resonance energy transfer HEK293 Human Not specified Human Full length 20935634
DD FADD Link MALT1 Co-immunoprecipitation Jurkat A3 15746428
DD FADD Link MALT1 Co-immunoprecipitation Caspase8 deficient Jurkat I9.2 15746428
DD FADD Link Myd88 Co-immunoprecipitation HEK293 Not used Human Full length 10880445
DD FADD Link Myd88 Co-immunoprecipitation HEK293 Not used Human 1-151 10880445
DD FADD Link Myd88 Co-immunoprecipitation Drosophila S2 Drosophila Not specified Drosophila 1-237 11606776
DD FADD Link Myd88 Co-immunoprecipitation BALB/C Mouse peritoneal macrophage 21131052
DD FADD Link PIDD Co-immunoprecipitation COS-7 Not specified Not specified Not specified 1-753 10825539
DD FADD Link PIDD Co-immunoprecipitation HEK293 Not used Not specified Not specified 16360037
DD FADD Link RIP Co-Immunofluorescence staining HEK293 Not specified Not specified Human Not specified 11002422
DD FADD Link RIP Co-immunoprecipitation HeLa Not specified 19-753 Not specified 261-372 8642271
DD FADD Link TNFR1 Rat primary hepatocyte 21741934
DD FADD Link TNFR1 Co-immunoprecipitation HEK293 Not specified 2-208 Not specified Not specified 8617770
DD FADD Link TNFR1 Co-immunoprecipitation HEK293 Not specified 80-208 Not specified Not specified 8617770
DD FADD Link TNFR1 Co-immunoprecipitation L929 10463949
DD FADD Link TNFR1 Co-immunoprecipitation Jurkat Not specified Not specified Not specified Not specified 11002422
DD FADD Link TRADD Co-Immunofluorescence staining Human TLE hammacampus section 16919273
DD FADD Link TRADD Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 8565075
DD FADD Link TRADD Co-immunoprecipitation HeLa Not specified 19-753 Not specified 195-312 8642271
DD FADD Link TRADD Co-immunoprecipitation HeLa 10521396
DD FADD Link TRADD Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 10521396
DD FADD Link TRADD Co-immunoprecipitation COS-7 Not specified Not specified Not specified Not specified 12796506
DD FADD Link TRADD Co-immunoprecipitation Human TLE hammacampus lysate 16919273
DD FADD Link TRADD Co-immunoprecipitation Mouse seizure hippocampal 21760970
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DD FADD Link Fas Mass spectrometry, MALS 5:5, 5:6, 5:7 20935634
DD FADD Link Fas Co-immunoprecipitation V238N, D244V, Q257X, A241D, I294S (Fas) 10200300
DD FADD Link Fas Co-immunoprecipitation R113E, D123R, R166E, Q169A, D175R, D106A, K125A, R142A (FADD) 14573612
DD FADD Link Fas Fluorescence resonance energy transfer D260V, D260Y, A257D, E261K, T270K, E272K, Q283K (Fas) R117E, D123R, R135E, R172E (FADD) 20935634
DD FADD Link Fas GST fusion protein pull-down T225P, Q257X, A241D, I294S, D244V, R234Q, L278X, R234P, Q260X, I238N (Fas) 10200300
DD FADD Link Fas GST fusion protein pull-down R113E, R117E, D123R, R166E, L172E, D175R, D106A, K125A, R142A, R146A (FADD) 14573612
DD FADD Link Fas Structure 4:4 L172A (FADD) 3EZQ 19118384
DD FADD Link Fas His fusion protein pull-down E261K, E272K, T270K, R250E, Q283K, K287D, R250Q, D260Y, A257D, E272K, T270K (fas) 20935634
DD FADD Link Fas Structure 5:5 3OQ9 20935634
DD FADD Link Fas Surface plasmon resonance 1.0E+07M-1 R110A, R113A, R117A, E118A, V121N, E123A (FADD) 10347191
DD FADD Link Fas Yeast two-hybrid R117A (FADD) 12107169
DD FADD Link Fas Yeast two-hybrid V108E(FADD) 15173180
DD FADD Link RIP1 GST fusion protein pull-down Q169A, N171A (FADD) G595A, K596A, E620A, D622A (RIP1) 22922561
DD FADD Link RIP1 Surface plasmon resonance 323nM 22922561
DD FADD Link TRADD GST fusion protein pull-down D246R, E255R, Q267A, R278A (TRADD) R113E, R117E, D123R, D175R (FADD) 16006552
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DD FADD Link DR4 β-galactosidase staining Apoptosis (DISC) 9430227
DD FADD Link DR4 Cytoplasmic histone associated DNA fragment detection Apoptosis (DISC) 9430228
DD FADD Link DR4 Caspase cleaved assay Caspase8 activation 15452120
DD FADD Link DR4 Immunofluorescence staining Apoptosis (DISC) 21272366
DD FADD Link DR4 Annexin V staining Apoptosis (DISC) 12920112
DD FADD Link DR5 β-galactosidase staining Apoptosis (DISC) 9430227
DD FADD Link DR5 Cytoplasmic histone associated DNA fragment detection Apoptosis (DISC) 9430228
DD FADD Link DR5 Caspase cleaved assay Caspase8 activation 15452120
DD FADD Link DR5 Immunofluorescence staining Apoptosis (DISC) 21272366
DD FADD Link DR5 Annexin V staining Apoptosis (DISC) 12920112
DD FADD Link Fas MTT assay Apoptosis (DISC) 21557211
DD FADD Link Fas MTT assay Apoptosis (DISC) 8617770
DD FADD Link Fas Propidium iodide staining Apoptosis (DISC) 8649383
DD FADD Link Fas Propidium iodide staining Apoptosis (DISC) 9325306
DD FADD Link Fas Propidium iodide staining Apoptosis (DISC) 10607716
DD FADD Link Fas Propidium iodide staining Apoptosis (DISC) 10919673
DD FADD Link RIP Propidium iodide staining Apoptosis (DISC) 11002422
DD FADD Link TNFR1 MTT assay Apoptosis (DISC) 8617770
(Link: click this icon to show interactions only between the two corresponding DDs)