FADD (DED)

Full Name:
Protein FADD
Alternative Names:
GIG3, MORT1, MGC8528
Function:
Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
Death Domain Subfamily & Boundary Region:
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq Q13158external db link DIP-286Nexternal db link Q13158external db link hsa:8772external db link Q13158external db link MINT-91814external db link
Mouse Seq Q61160external db link DIP-34771Nexternal db link Q61160external db link mmu:14082external db link Q61160external db link MINT-1514991external db link
Rat Seq Q8R2E7external db link Q8R2E7external db link rno:266610external db link
Structure Species Residue Method Expression Purification Column Final condition Final concentration Reference
1A1W Download Human 1-83 NMR E.coli Affinity chromatography 50mM sodium acetate(pH4.0), 50mM nacl 0.7mM 9582077
2GF5 Download Human 2-191 NMR E.coli 50mM potassium phosphate(pH4.0), 150mM NaCl, 1mM DTT 1.5mM 10964568
Disease Mutation in disease Reference
Infections, recurrent with encephalopathy, hepatic dysfunction and cardiovasuclar malforamtions C105W 21109225
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DED FADD Link Caspase10 GST fusion protein pull-down Not specified Full length E.coli HuH-7 lysate 12407100
DED FADD Link Caspase10 His fusion protein pull-down Not specified Not specified Not specified Human Not specified In vitro translation 9045686
DED FADD Link Caspase8 Fluorescence resonance energy transfer ` 11717445
DED FADD Link Caspase8 GST fusion protein pull-down Not specified Full length E.coli HuH-7 lysate 12407100
DED FADD Link Caspase8 His fusion protein pull-down Not specified Full length Not specified Not specified 1-479 In vitro translation 8681377
DED FADD Link Caspase8 His fusion protein pull-down Not specified Not specified Not specified Human Not specified In vitro translation 9045686
DED FADD Link Caspase8 His fusion protein pull-down Not specified Not specified In vitro translation Human 1-479 In vitro translation 12010809
DED FADD Link Caspase8 In vitro purification protein assembly(2D gel electrophoresis) Not specified Full length HEK293 Not specified 1-479 In vitro translation 8681377
DED FADD Link Caspase8 Yeast two-hybrid Human Full length Yeast Human Not specified Yeast 8681376
DED FADD Link Caspase8 Yeast two-hybrid Human 1-79 Yeast Human Not specified Yeast 8681376
DED FADD Link Caspase8 Yeast two-hybrid Mouse 1-96 Yeast Mouse 1-202 Yeast 10526240
DED FADD Link Caspase8 Yeast two-hybrid Not specified Not specified Yeast Human Full length Yeast 15782135
DED FADD Link DEDD GST fusion protein pull-down Not specified Full length In vitro translation Human 1-318 In vitro translation 9774341
DED FADD Link DEDD GST fusion protein pull-down Not specified 1-88 In vitro translation Human 1-318 In vitro translation 9774341
DED FADD Link FLIP GST fusion protein pull-down Not specified 1-79 In vitro translation Human Full length Not specified 9228018
DED FADD Link FLIP GST fusion protein pull-down Not specified Full length E.coli HuH-7 lysate 12407100
DED FADD Link FLIP Yeast two-hybrid Human Full length Yeast Human 1-215 Yeast 9208847
DED FADD Link FLIP Yeast two-hybrid Not specified 1-79 Yeast Human 1-160 Yeast 9228018
DED FADD Link FLIP Yeast two-hybrid Not specified Not specified Yeast Human Full length Yeast 9289491
DED FADD Link FLIP Yeast two-hybrid Not specified 1-208 In vitro translation Human 1-180 Not specified 9326610
DED FADD Link PEA15 GST fusion protein pull-down Not specified Not specified In vitro translation Not specified Not specified In vitro translation 10493725
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DED FADD Link Caspase10 Co-immunoprecipitation HEK293 Not specified Not specified Human Not specified 9045686
DED FADD Link Caspase10 Fluorescence resonance energy transfer HEK293 Not specified Not specified Not specified Full length 11717445
DED FADD Link Caspase8 HSC3 21525013
DED FADD Link Caspase8 Co-immunoprecipitation HEK293 Human Not specified Human Not specified 8681376
DED FADD Link Caspase8 Co-immunoprecipitation T47D Human Not specified Not specified Not specified 9582077
DED FADD Link Caspase8 Co-immunoprecipitation Jurkat A3 11432804
DED FADD Link Caspase8 Co-immunoprecipitation MCF-7 Not used Human 1-276 12010809
DED FADD Link Caspase8 Co-immunoprecipitation HEK293 Human 2-208 Human 2-177 12220669
DED FADD Link Caspase8 Co-immunoprecipitation HT1080 12887920
DED FADD Link Caspase8 Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 15701651
DED FADD Link Caspase8 Co-immunoprecipitation SKW6.4 17159907
DED FADD Link Caspase8 Co-immunoprecipitation H2009 18485876
DED FADD Link Caspase8 Co-immunoprecipitation Panc-1 18485876
DED FADD Link Caspase8 Co-immunoprecipitation HT-1080 19060883
DED FADD Link Caspase8 Co-immunoprecipitation HT-29 19524512
DED FADD Link Caspase8 Co-immunoprecipitation H2009 20547836
DED FADD Link Caspase8 Co-immunoprecipitation SH-EP 22890322
DED FADD Link Caspase8 Co-immunoprecipitation LAN-5 22890322
DED FADD Link Caspase8 Fluorescence resonance energy transfer HEK293 Not specified Not specified Not specified Full length 11717445
DED FADD Link DEDD Co-immunoprecipitation HEK293 Not used Human 1-318 9774341
DED FADD Link DEDD GST fusion protein pull-down HEK293 Not specified Not specified Not specified Not specified 11741985
DED FADD Link FLIP Co-immunoprecipitation HEK293 Not specified Not specified Human 1-195 9208847
DED FADD Link FLIP Co-immunoprecipitation HEK293 Not specified Not specified Human 78-480 9208847
DED FADD Link FLIP Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 9228018
DED FADD Link FLIP Co-immunoprecipitation U937 11859150
DED FADD Link FLIP Co-immunoprecipitation Raji cell Not used Not specified Not specified 12215447
DED FADD Link FLIP Co-immunoprecipitation HEK293 Human 2-208 Human 1-171 12220669
DED FADD Link FLIP Co-immunoprecipitation HEK293 Human 2-208 Human Full length 12220669
DED FADD Link PEA15 Co-immunoprecipitation HeLa Human 1-208 Human Full length 10442631
DED FADD Link PEA15 Co-immunoprecipitation LNCaP 22179576
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DED FADD Link Caspase8 Co-immunoprecipitation F25G, F35V (FADD) 9582077
DED FADD Link Caspase8 Co-immunoprecipitation K33Q and K35Q, F25G (FADD) 12220669
DED FADD Link FLIP Co-immunoprecipitation K33Q and K35Q, F25G, F25Y, K33N and K35N (FADD) 12220669
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DED FADD Link Caspase10 DAPI staining Apoptosis (DISC) 9045686
DED FADD Link Caspase8 β-galactosidase staining Apoptosis (DISC) 8681376
DED FADD Link Caspase8 DNA fragmentation assay Apoptosis (DISC) 11432804
DED FADD Link Caspase8 Propidium iodide staining Apoptosis (DISC) 12010809
DED FADD Link Caspase8 Hoechst staining Apoptosis (DISC) 12887920
DED FADD Link Caspase8 PARP cleaved assay Apoptosis (DISC) 18485876
DED FADD Link Caspase8 MTT assay Apoptosis (DISC) 22890322
DED FADD Link Caspase8 DAPI staining Apoptosis (DISC) 9045686
DED FADD Link DEDD Data not shown Apoptosis 9774341
DED FADD Link FLIP β-galactosidase staining Apoptosis inhibition 9208847
DED FADD Link FLIP Annexin V staining Apoptosis inhibition 11859150
DED FADD Link FLIP Confocal microscopy Apoptosis inhibition 12215447
DED FADD Link PEA15 DAPI, Annexin V staining, TUNEL assay Apoptosis inhibition 10493725
(Link: click this icon to show interactions only between the two corresponding DDs)