FLIP (DED)

Full Name:
CASP8 and FADD-like apoptosis regulator
Alternative Names:
CASH, FLIP, MRIT, CLARP, FLAME, Casper, FLAME1, c-FLIP, FLAME-1, I-FLICE, c-FLIPL, c-FLIPR, c-FLIPS, CASP8AP1, cflar
Function:
Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
Death Domain Subfamily & Boundary Region:
  • DED : 1-73, 92-170 Seq
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq O15519external db link DIP-27629Nexternal db link O15519external db link hsa:8837external db link O15519external db link MINT-1529273external db link
Mouse Seq O35732external db link O35732external db link mmu:12633external db link O35732external db link
Rat Seq Q99MZ5external db link Q99MZ5external db link rno:117279external db link
Dog Seq E2RR61external db link
Zebra fish Seq Q1JPW7external db link Q1JPW7external db link
Structure Species Residue Method Expression Purification Column Final condition Final concentration Reference
2BBR Download Molluscum contagiosum virus subtype 1? 1-187 X-ray E.coli Gel filtration 16364918
2F1S Download Molluscum contagiosum virus subtype 1 1-183 X-ray E.coli Gel filtration Superdex S200 16317000
No information
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DED FLIP Link Caspase10 GST fusion protein pull-down Human Full length Not specified Not specified Not specified In vitro translation 9228018
DED FLIP Link Caspase10 Yeast two-hybrid Human 1-215 Yeast Human Full length Yeast 9208847
DED FLIP Link Caspase10 Yeast two-hybrid Human 1-160 Yeast Not specified 18-189 Yeast 9228018
DED FLIP Link Caspase8 GST fusion protein pull-down Human Full length Not specified Not specified 1-201 In vitro translation 9228018
DED FLIP Link Caspase8 Yeast two-hybrid Human 1-215 Yeast Human Full length Yeast 9208847
DED FLIP Link Caspase8 Yeast two-hybrid Human 1-160 Yeast Not specified 1-201 Yeast 9228018
DED FLIP Link Caspase8 Yeast two-hybrid Human Full length Yeast Not specified Not specified Yeast 9289491
DED FLIP Link Caspase8 Yeast two-hybrid Human 1-208 Not specified Not specified 1-480 In vitro translation 9326610
DED FLIP Link Caspase8 Yeast two-hybrid Human 1-180 Not specified Not specified 1-216 In vitro translation 9326610
DED FLIP Link DEDD GST fusion protein pull-down Human Full length In vitro translation Not specified Full length E.coli 11965497
DED FLIP Link DEDD2 GST fusion protein pull-down Not specified Not specified In vitro translation Human Not specified E.coli 11741985
DED FLIP Link DEDD2 GST fusion protein pull-down Human Full length In vitro translation Not specified Full length E.coli 11965497
DED FLIP Link FADD GST fusion protein pull-down Human Full length Not specified Not specified 1-79 In vitro translation 9228018
DED FLIP Link FADD GST fusion protein pull-down HuH-7 lysate Not specified Full length E.coli 12407100
DED FLIP Link FADD Yeast two-hybrid Human 1-215 Yeast Human Full length Yeast 9208847
DED FLIP Link FADD Yeast two-hybrid Human 1-160 Yeast Not specified 1-79 Yeast 9228018
DED FLIP Link FADD Yeast two-hybrid Human Full length Yeast Not specified Not specified Yeast 9289491
DED FLIP Link FADD Yeast two-hybrid Human 1-180 Not specified Not specified 1-208 In vitro translation 9326610
DED FLIP Link FLIP Yeast two-hybrid Human 1-160 Yeast Human 1-160 Yeast 9228018
DED FLIP Link FLIP Yeast two-hybrid Human Full length Yeast Human Full length Yeast 9289491
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DED FLIP Link Caspase10 Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 9211860
DED FLIP Link Caspase10 Co-immunoprecipitation HEK293 Human Full length Not specified 18-189 9228018
DED FLIP Link Caspase10 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 12887920
DED FLIP Link Caspase8 Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 9208847
DED FLIP Link Caspase8 Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 9211860
DED FLIP Link Caspase8 Co-immunoprecipitation HEK293 Human Full length Not specified 1-201 9228018
DED FLIP Link Caspase8 Co-immunoprecipitation HEK293 Not specified Not specified Human Not specified 9380701
DED FLIP Link Caspase8 Co-immunoprecipitation Raji cell Not specified Not specified Not used 12215447
DED FLIP Link Caspase8 Co-immunoprecipitation HT1080 12887920
DED FLIP Link Caspase8 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 12887920
DED FLIP Link Caspase8 Co-immunoprecipitation SKW6.4 17159907
DED FLIP Link DEDD GST fusion protein pull-down HEK293 Not specified Not specified Not specified Not specified 11741985
DED FLIP Link DEDD2 Co-Immunofluorescence staining MCF-7 Not specified Not specified Not specified Full length 11965497
DED FLIP Link DEDD2 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 11741985
DED FLIP Link DEDD2 Co-immunoprecipitation HEK293 Human Full length Not specified Full length 11965497
DED FLIP Link FADD Co-immunoprecipitation HEK293 Human 1-195 Not specified Not specified 9208847
DED FLIP Link FADD Co-immunoprecipitation HEK293 Human 78-480 Not specified Not specified 9208847
DED FLIP Link FADD Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 9228018
DED FLIP Link FADD Co-immunoprecipitation U937 11859150
DED FLIP Link FADD Co-immunoprecipitation Raji cell Not specified Not specified Not used 12215447
DED FLIP Link FADD Co-immunoprecipitation HEK293 Human 1-171 Human 2-208 12220669
DED FLIP Link FADD Co-immunoprecipitation HEK293 Human Full length Human 2-208 12220669
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DED FLIP Link FADD Co-immunoprecipitation K33Q and K35Q, F25G, F25Y, K33N and K35N (FADD) 12220669
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DED FLIP Link Caspase10 β-galactosidase staining Apoptosis inhibition 9228018
DED FLIP Link Caspase8 β-galactosidase staining Apoptosis inhibition 9228018
DED FLIP Link Caspase8 Confocal microscopy Apoptosis inhibition 12215447
DED FLIP Link FADD β-galactosidase staining Apoptosis inhibition 9208847
DED FLIP Link FADD Annexin V staining Apoptosis inhibition 11859150
DED FLIP Link FADD Confocal microscopy Apoptosis inhibition 12215447
(Link: click this icon to show interactions only between the two corresponding DDs)