IRAK1 (DD)

Full Name:
Interleukin-1 receptor-associated kinase 1
Alternative Names:
IRAK, pelle
Function:
Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.
Death Domain Subfamily & Boundary Region:
  • DD : 29-102 Seq
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq P51617external db link DIP-397Nexternal db link P51617external db link hsa:3654external db link P51617external db link MINT-97088external db link
Mouse Seq Q62406external db link DIP-31491Nexternal db link Q62406external db link mmu:16179external db link Q62406external db link
Rat Seq F1LSW4external db link
Dog Seq E2QX24external db link
Zebra fish Seq A8WIP4external db link A8WIP4external db link
Not solved yet
No information
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD IRAK1 Link IRAK1 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 10383454
DD IRAK1 Link IRAK1 Co-immunoprecipitation HEK293 Human Full length Human 2-109 17276401
DD IRAK1 Link IRAK1 Co-immunoprecipitation HEK293 Mouse 1-550 Mouse 1-550 19166926
DD IRAK1 Link IRAK2 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 10383454
DD IRAK1 Link IRAK2 Fluorescence resonance energy transfer NIH 3T3 Not specified Not specified Not specified Full length 12138165
DD IRAK1 Link IRAK3 Co-immunoprecipitation HEK293 Not specified Full length Human Full length 10383454
DD IRAK1 Link IRAK3 Co-immunoprecipitation HEK293 Mouse 1-550 Not specified Not specified 19166926
DD IRAK1 Link IRAK3 Co-immunoprecipitation Mouse RAW 264.7 macrophage Mouse 1-550 Not specified Not specified 19166926
DD IRAK1 Link IRAK3 Fluorescence resonance energy transfer NIH 3T3 Not specified Not specified Human Not specified 12138165
DD IRAK1 Link IRAK4 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Full length 12538665
DD IRAK1 Link IRAK4 Co-immunoprecipitation HEK293 Not used Human Not specified 15905496
DD IRAK1 Link IRAK4 Co-immunoprecipitation HEK293 Human Not specified Human Not specified 16024789
DD IRAK1 Link IRAK4 Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 17276401
DD IRAK1 Link IRAK4 Co-immunoprecipitation HEK293 Human 2-109 Not specified Not specified 17276401
DD IRAK1 Link IRAK4 Co-immunoprecipitation HEK293 Human 2-712 Not specified Not specified 18524972
DD IRAK1 Link Myd88 Co-immunoprecipitation HEK293 Not specified Full length Not specified 1-151 9430229
DD IRAK1 Link Myd88 Co-immunoprecipitation HEK293 Not specified Full length Not specified 1-151 10383454
DD IRAK1 Link Myd88 Co-immunoprecipitation THP-1 10811644
DD IRAK1 Link Myd88 Co-immunoprecipitation HEK293 Not specified Not specified Not specified 1-172 10854325
DD IRAK1 Link Myd88 Co-immunoprecipitation HEK293 Human 1-215 Human Full length 11960013
DD IRAK1 Link Myd88 Co-immunoprecipitation HEK293 11976320
DD IRAK1 Link Myd88 Co-immunoprecipitation PC12 12034707
DD IRAK1 Link Myd88 Co-immunoprecipitation HEK293 Human Not specified Human Not specified 16024789
DD IRAK1 Link Myd88 Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 17276401
DD IRAK1 Link Myd88 Co-immunoprecipitation HEK293 Human 2-109 Not specified Not specified 17276401
DD IRAK1 Link Myd88 Co-immunoprecipitation Rat thoracic aortas derived vascular smooth muscle cell Not specified 1-710 Not specified 1-306 17510469
DD IRAK1 Link Myd88 Co-immunoprecipitation MT2 17920759
DD IRAK1 Link Myd88 Co-immunoprecipitation HUT102 17920759
DD IRAK1 Link Myd88 Co-immunoprecipitation MT4 17920759
DD IRAK1 Link Myd88 Co-immunoprecipitation HEK293 Human 2-712 Not specified Not specified 18524972
DD IRAK1 Link Myd88 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 19679662
DD IRAK1 Link Myd88 Co-immunoprecipitation HEK293 Not specified 27-72 Not specified Not specified 19679662
DD IRAK1 Link Myd88 Co-immunoprecipitation HEK293 Not specified Not specified Not specified 27-72 19679662
DD IRAK1 Link Myd88 Co-immunoprecipitation GCB DLBCL line BAJB Not used Not specified Not specified 21179087
DD IRAK1 Link NGFR Co-immunoprecipitation PC12 12034707
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DD IRAK1 Link Myd88 Co-immunoprecipitation E52A and E53A, Y58A, K95A (Myd88) 19679662
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DD IRAK1 Link IRAK2 Kinase assay IRAK2 activation 10383454
DD IRAK1 Link IRAK3 Kinase assay IRAK3 activation 10383454
DD IRAK1 Link IRAK4 Kinase assay IRAK1 phosphorylation 16024789
DD IRAK1 Link Myd88 Luciferase assay NF-kB activation 9430229
DD IRAK1 Link Myd88 Luciferase assay NF-kB-activation 19679662
DD IRAK1 Link NGFR Luciferase assay NF-kB activation 12034707
(Link: click this icon to show interactions only between the two corresponding DDs)