IRAK4 (DD)

Full Name:
Interleukin-1 receptor-associated kinase 4
Alternative Names:
IPD1, REN64, NY-REN-64
Function:
Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.
Death Domain Subfamily & Boundary Region:
  • DD : 20-104 Seq
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq Q9NWZ3external db link DIP-31351Nexternal db link Q9NWZ3external db link hsa:51135external db link Q9NWZ3external db link MINT-1383671external db link
Mouse Seq Q8R4K2external db link DIP-48996Nexternal db link Q8R4K2external db link mmu:266632external db link Q8R4K2external db link
Rat Seq D4A7K4external db link
Dog Seq E2RDL0external db link
Zebra fish Seq Q6IWL3external db link Q6IWL3external db link
Structure Species Residue Method Expression Purification Column Final condition Final concentration Reference
3MOP Download Human 4-106 X-ray E.coli Gel filtration Superdex 200 1mg/ml 20485341
1WH4 Download Mouse NMR
2A9I Download Mouse 1-113 X-ray E.coli Affinity chromatography 20mM HEPES(pH7.5), 125mM KCl 10mg/ml 16177054
Disease Mutation in disease Reference
Invasive pneumococcal disease 16950813
Irak4 deficiency Q293X, 287del 12637671
Irak4 deficiency Q293X 17114497
Irak4 deficiency R12C 17878374
Irak4 deficiency Q293X 12925671
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD IRAK4 Link Myd88 His fusion protein pull-down Human 1-185 E.coli Human 1-157 E.coli 19167362
DD IRAK4 Link Myd88 In vitro purification protein assembly(chemical crosslinking) Human 1-152 E.coli Human 1-110 E.coli 19592493
DD IRAK4 Link Myd88 In vitro purification protein assembly(Size-exclusion chromatography) Human 1-152 E.coli Human 1-110 E.coli 19592493
DD IRAK4 Link Myd88 In vitro purification protein assembly(Size-exclusion chromatography) Human 1-117 E.coli Human 1-157 E.coli 20966070
DD IRAK4 Link Myd88 Yeast two-hybrid Not specified Full length Yeast Not specified Full length Yeast 12538665
DD IRAK4 Link Myd88 Yeast two-hybrid Not specified Full length Yeast Not specified 1-172 Yeast 12538665
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD IRAK4 Link IRAK1 Co-immunoprecipitation HEK293 Not specified Full length Not specified Not specified 12538665
DD IRAK4 Link IRAK1 Co-immunoprecipitation HEK293 Human Not specified Not used 15905496
DD IRAK4 Link IRAK1 Co-immunoprecipitation HEK293 Human Not specified Human Not specified 16024789
DD IRAK4 Link IRAK1 Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 17276401
DD IRAK4 Link IRAK1 Co-immunoprecipitation HEK293 Not specified Not specified Human 2-109 17276401
DD IRAK4 Link IRAK1 Co-immunoprecipitation HEK293 Not specified Not specified Human 2-712 18524972
DD IRAK4 Link IRAK2 His fusion protein pull-down E.coli Human 4-106 Human 1-112 20485341
DD IRAK4 Link Myd88 Co-immunoprecipitation HEK293 Human 1-215 Human Full length 11960013
DD IRAK4 Link Myd88 Co-immunoprecipitation HEK293 Human Not specified Not specified Not specified 15905496
DD IRAK4 Link Myd88 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 19679662
DD IRAK4 Link Myd88 Co-immunoprecipitation HEK293 Human 1-117 Human 1-157 20966070
DD IRAK4 Link Myd88 Co-immunoprecipitation GCB DLBCL line BAJB Not used Not specified Not specified 21179087
DD IRAK4 Link Myd88 His fusion protein pull-down E.coli Human 4-106 Human 20-117 20485341
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DD IRAK4 Link IRAK2 Structure 6:4:4 Y6D, W62D, M66D, R67D (IRAK2) 3MOP 20485341
DD IRAK4 Link Myd88 Co-immunoprecipitation E52A and E53A, Y58A, K95A (Myd88) 19679662
DD IRAK4 Link Myd88 Co-immunoprecipitation S34Y, R98C (Myd88) 20966070
DD IRAK4 Link Myd88 Structure 6:4:4 N16E and R20E, E69K, T76E, N78E, R12A (IRAK4) V43D, E52K, I61A, Y58A and R62A, R62E, A44E (Myd88) 3MOP 20485341
DD IRAK4 Link Myd88 Gel filtration 4:7, 4:8 19592493
DD IRAK4 Link Myd88 Gel filtration 7:4, 8:4 19592493
DD IRAK4 Link Myd88 In vitro purification protein assembly(Size-exclusion chromatography) S34Y, R98C (Myd88) 20966070
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DD IRAK4 Link IRAK1 Kinase assay IRAK1 phosphorylation 16024789
DD IRAK4 Link Myd88 Luciferase assay NF-kB activation (Myddosome) 20966070
DD IRAK4 Link Myd88 Kinase assay IRAK4 mediated IRAK1 phosphorylation 12538665
(Link: click this icon to show interactions only between the two corresponding DDs)