MADD (DD)

Full Name:
MAP kinase-activating death domain protein
Alternative Names:
DENN, IG20, RAB3GEP, FLJ35600, FLJ36300, KIAA0358
Function:
Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows decreased. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis); isoform 1 is susceptible to inducing apoptosis, isoform 5 is resistant and isoform 3 and isoform 4 have no effect.
Death Domain Subfamily & Boundary Region:
  • DD : 1340-1415 Seq
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq Q8WXG6external db link DIP-33966Nexternal db link Q8WXG6external db link hsa:8567external db link Q8WXG6external db link
Mouse Seq Q80U28external db link Q80U28external db link mmu:228355external db link
Rat Seq O08873external db link O08873external db link rno:94193external db link
Dog Seq E2QTJ4external db link cfa:475982external db link
Zebra fish Seq E7F6X3external db link
Not solved yet
No information
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD MADD Link MADD Yeast two-hybrid Not specified 1281-1356 Yeast Not specified 1281-1356 Yeast 9115275
DD MADD Link TNFR1 GST fusion protein pull-down Not specified 982-1588 Not specified Not specified 326-413 Not specified 9115275
DD MADD Link TNFR1 Yeast two-hybrid Not specified 982-1588 Yeast Not specified 326-413 Yeast 9115275
DD MADD Link TNFR1 Yeast two-hybrid Not specified 1281-1356 Yeast Not specified 326-413 Yeast 9115275
DD MADD Link TRADD Yeast two-hybrid Not specified 1281-1356 Yeast Not specified 222-289 Yeast 9115275
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD MADD Link DR4 Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 15208670
DD MADD Link DR4 Co-immunoprecipitation HEK293 Human 628-1376 Not specified Not specified 15208670
DD MADD Link DR4 Fluorescence resonance energy transfer HeLa Human Full length Not specified Not specified 15208670
DD MADD Link DR5 Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 15208670
DD MADD Link DR5 Co-immunoprecipitation HEK293 Human 628-1376 Not specified Not specified 15208670
DD MADD Link PIDD Co-immunoprecipitation COS-7 Not specified Not specified Not specified 1-753 10825539
DD MADD Link TNFR1 Co-immunoprecipitation COS-7 Not specified Full length Not used 9115275
DD MADD Link TNFR1 Co-immunoprecipitation CCL-131 Not specified Not specified Not specified Not specified 15007167
DD MADD Link TRADD Co-immunoprecipitation CCL-131 Not specified Not specified Not specified Not specified 15007167
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DD MADD Link DR4 Immunofluorescence staining Apoptosis (DISC) 15208670
DD MADD Link DR5 Immunofluorescence staining Apoptosis (DISC) 15208670
DD MADD Link TRADD Annexin V staining, Immunoprecipitation Apoptosis inhibition, TRADD-TNFR1 interaction block 15007167
(Link: click this icon to show interactions only between the two corresponding DDs)