Myd88 (DD)

Full Name:
Myeloid differentiation primary response protein MyD88
Alternative Names:
MYD88D
Function:
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
Death Domain Subfamily & Boundary Region:
  • DD : 54-109 Seq
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq Q99836external db link DIP-31349Nexternal db link Q99836external db link hsa:4615external db link Q99836external db link MINT-97233external db link
Mouse Seq P22366external db link P22366external db link mmu:17874external db link P22366external db link
Rat Seq Q6Y1S1external db link Q6Y1S1external db link rno:301059external db link
Dog Seq F1P7V2external db link
Zebra fish Seq Q5XJ85external db link Q5XJ85external db link dre:403145external db link
Structure Species Residue Method Expression Purification Column Final condition Final concentration Reference
3MOP Download Human 20-117 X-ray E.coli Gel filtration Superdex 200 1mg/ml 20485341
Disease Mutation in disease Reference
Myd88 deficiency L93P, R196C, E52del 18669862
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD Myd88 Link IRAK2 GST fusion protein pull-down Not specified 1-296 E.coli Not specified Full length In vitro translation 12659850
DD Myd88 Link IRAK2 GST fusion protein pull-down Not specified 1-109 E.coli Not specified Full length In vitro translation 12659850
DD Myd88 Link IRAK2 GST fusion protein pull-down Not specified 1-155 E.coli Not specified Full length In vitro translation 12659850
DD Myd88 Link IRAK4 His fusion protein pull-down Human 1-157 E.coli Human 1-185 E.coli 19167362
DD Myd88 Link IRAK4 In vitro purification protein assembly(chemical crosslinking) Human 1-110 E.coli Human 1-152 E.coli 19592493
DD Myd88 Link IRAK4 In vitro purification protein assembly(Size-exclusion chromatography) Human 1-110 E.coli Human 1-152 E.coli 19592493
DD Myd88 Link IRAK4 In vitro purification protein assembly(Size-exclusion chromatography) Human 1-157 E.coli Human 1-117 E.coli 20966070
DD Myd88 Link IRAK4 Yeast two-hybrid Not specified Full length Yeast Not specified Full length Yeast 12538665
DD Myd88 Link IRAK4 Yeast two-hybrid Not specified 1-172 Yeast Not specified Full length Yeast 12538665
DD Myd88 Link Myd88 GST fusion protein pull-down Mouse Full length Not specified Mouse Full length In vitro translation 9575168
DD Myd88 Link Myd88 Yeast two-hybrid Mouse Full length Yeast Mouse Full length Yeast 9575168
DD Myd88 Link Myd88 Yeast two-hybrid Not specified Full length Yeast Not specified Full length Yeast 11544529
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD Myd88 Link FADD Co-immunoprecipitation HEK293 Human Full length Not used 10880445
DD Myd88 Link FADD Co-immunoprecipitation HEK293 Human 1-151 Not used 10880445
DD Myd88 Link FADD Co-immunoprecipitation Drosophila S2 Drosophila 1-237 Drosophila Not specified 11606776
DD Myd88 Link FADD Co-immunoprecipitation BALB/C Mouse peritoneal macrophage 21131052
DD Myd88 Link IRAK1 Co-immunoprecipitation HEK293 Not specified 1-151 Not specified Full length 9430229
DD Myd88 Link IRAK1 Co-immunoprecipitation HEK293 Not specified 1-151 Not specified Full length 10383454
DD Myd88 Link IRAK1 Co-immunoprecipitation THP-1 10811644
DD Myd88 Link IRAK1 Co-immunoprecipitation HEK293 Not specified 1-172 Not specified Not specified 10854325
DD Myd88 Link IRAK1 Co-immunoprecipitation HEK293 Human Full length Human 1-215 11960013
DD Myd88 Link IRAK1 Co-immunoprecipitation HEK293 11976320
DD Myd88 Link IRAK1 Co-immunoprecipitation PC12 12034707
DD Myd88 Link IRAK1 Co-immunoprecipitation HEK293 Human Not specified Human Not specified 16024789
DD Myd88 Link IRAK1 Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 17276401
DD Myd88 Link IRAK1 Co-immunoprecipitation HEK293 Not specified Not specified Human 2-109 17276401
DD Myd88 Link IRAK1 Co-immunoprecipitation Rat thoracic aortas derived vascular smooth muscle cell Not specified 1-306 Not specified 1-710 17510469
DD Myd88 Link IRAK1 Co-immunoprecipitation MT2 17920759
DD Myd88 Link IRAK1 Co-immunoprecipitation HUT102 17920759
DD Myd88 Link IRAK1 Co-immunoprecipitation MT4 17920759
DD Myd88 Link IRAK1 Co-immunoprecipitation HEK293 Not specified Not specified Human 2-712 18524972
DD Myd88 Link IRAK1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 19679662
DD Myd88 Link IRAK1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified 27-72 19679662
DD Myd88 Link IRAK1 Co-immunoprecipitation HEK293 Not specified 27-72 Not specified Not specified 19679662
DD Myd88 Link IRAK1 Co-immunoprecipitation GCB DLBCL line BAJB Not specified Not specified Not used 21179087
DD Myd88 Link IRAK2 Co-immunoprecipitation HEK293 Human Full length Human Full length 9374458
DD Myd88 Link IRAK2 Co-immunoprecipitation HEK293 Not specified 1-151 Not specified Full length 10383454
DD Myd88 Link IRAK2 His fusion protein pull-down E.coli Human 20-117 Human 1-112 20485341
DD Myd88 Link IRAK3 Co-immunoprecipitation HEK293 Not specified 1-151 Human Full length 10383454
DD Myd88 Link IRAK4 Co-immunoprecipitation HEK293 Human Full length Human 1-215 11960013
DD Myd88 Link IRAK4 Co-immunoprecipitation HEK293 Not specified Not specified Human Not specified 15905496
DD Myd88 Link IRAK4 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 19679662
DD Myd88 Link IRAK4 Co-immunoprecipitation HEK293 Human 1-157 Human 1-117 20966070
DD Myd88 Link IRAK4 Co-immunoprecipitation GCB DLBCL line BAJB Not specified Not specified Not used 21179087
DD Myd88 Link IRAK4 His fusion protein pull-down E.coli Human 20-117 Human 4-106 20485341
DD Myd88 Link Myd88 Co-immunoprecipitation HEK293 Mouse 1-296 Mouse 1-296 11909531
DD Myd88 Link Myd88 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 19679662
DD Myd88 Link Myd88 Co-immunoprecipitation HEK293 Human 1-157 Human 1-157 20966070
DD Myd88 Link Myd88 Size-exclusion chromatography HEK293 Mouse Full length Mouse Full length 9575168
DD Myd88 Link NGFR Co-immunoprecipitation PC12 12034707
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DD Myd88 Link IRAK1 Co-immunoprecipitation E52A and E53A, Y58A, K95A (Myd88) 19679662
DD Myd88 Link IRAK2 Structure 6:4:4 3MOP 20485341
DD Myd88 Link IRAK4 Co-immunoprecipitation E52A and E53A, Y58A, K95A (Myd88) 19679662
DD Myd88 Link IRAK4 Co-immunoprecipitation S34Y, R98C (Myd88) 20966070
DD Myd88 Link IRAK4 Structure 6:4:4 N16E and R20E, E69K, T76E, N78E, R12A (IRAK4) V43D, E52K, I61A, Y58A and R62A, R62E, A44E (Myd88) 3MOP 20485341
DD Myd88 Link IRAK4 Gel filtration 4:7, 4:8 19592493
DD Myd88 Link IRAK4 Gel filtration 7:4, 8:4 19592493
DD Myd88 Link IRAK4 In vitro purification protein assembly(Size-exclusion chromatography) S34Y, R98C (Myd88) 20966070
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DD Myd88 Link IRAK1 Luciferase assay NF-kB activation 9430229
DD Myd88 Link IRAK1 Luciferase assay NF-kB-activation 19679662
DD Myd88 Link IRAK2 Luciferase assay NF-kB activation (Myddosome) 9374458
DD Myd88 Link IRAK4 Luciferase assay NF-kB activation (Myddosome) 20966070
DD Myd88 Link IRAK4 Kinase assay IRAK4 mediated IRAK1 phosphorylation 12538665
(Link: click this icon to show interactions only between the two corresponding DDs)