NF-kB2 (DD)

Full Name:
Nuclear factor NF-kappa-B p100 subunit
Alternative Names:
p52, LYT10, LYT-10, NFKB2
Function:
NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo-or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.
Death Domain Subfamily & Boundary Region:
  • DD : 764-851 Seq
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq Q00653external db link DIP-24239Nexternal db link Q00653external db link hsa:4791external db link Q00653external db link MINT-1131271external db link
Mouse Seq Q9WTK5external db link Q9WTK5external db link mmu:18034external db link Q9WTK5external db link
Rat Seq Q5U2Z4external db link Q5U2Z4external db link rno:309452external db link
Dog Seq E2RLL2external db link
Zebra fish Seq Q6K198external db link Q6K198external db link
Structure Species Residue Method Expression Purification Column Final condition Final concentration Reference
2D96 Download Human NMR
No information
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD NF-kB2 Link NF-kB1 Affinity purification-mass spectrometry HEK293 lysate HEK293 lysate 14743216
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD NF-kB2 Link NF-kB1 Co-immunoprecipitation U2OS 17962807
(Link: click this icon to show interactions only between the two corresponding DDs)