NLRP1 (CARD)

Full Name:
NACHT, LRR and PYD domains-containing protein 1
Alternative Names:
NAC, CARD7, NALP1, SLEV1, DEFCAP, PP1044, VAMAS1, CLR17.1, KIAA0926, DEFCAP-L/S, DKFZp586O1822
Function:
Able to form cytoplasmic structures termed death effector filaments. Enhances APAF1 and cytochrome c-dependent activation of pro-caspase-9 and consecutive apoptosis. Stimulates apoptosis through activation of caspase-3. Involved in activation of caspase-1 and caspase-5 as part of the NALP1 inflammasome complex which leads to processing and release of IL1B and IL18. Binds ATP.
Death Domain Subfamily & Boundary Region:
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq Q9C000external db link DIP-38407Nexternal db link Q9C000external db link hsa:22861external db link Q9C000external db link
Mouse Seq Q2LKU9external db link Q2LKU9external db link mmu:195046external db link
Rat Seq D9I2G4external db link
Dog Seq E2R3A5external db link
Structure Species Residue Method Expression Purification Column Final condition Final concentration Reference
3KAT Download Human X-ray
Disease Mutation in disease Reference
Vitiligo associated multiple autoimmune disease (VAMAS1) L155H 17377159
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
CARD NLRP1 Link Apaf1 GST fusion protein pull-down Not specified Not specified E.coli Not specified Not specified In vitro translation 11113115
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD NLRP1 Link Apaf1 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 11113115
CARD NLRP1 Link Apaf1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 17418785
CARD NLRP1 Link CARD12 Co-immunoprecipitation HEK293 Not specified Not specified Human Not specified 11472070
CARD NLRP1 Link CARD6 Co-immunoprecipitation HEK293 Not specified Full length Human 1-311 12775719
CARD NLRP1 Link Caspase1 Co-immunoprecipitation HEK293 Human Full length Human Not specified 17349957
CARD NLRP1 Link Caspase1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 17418785
CARD NLRP1 Link Caspase1 Co-immunoprecipitation Rat cortical lysate 19401709
CARD NLRP1 Link Caspase1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 21252346
CARD NLRP1 Link Caspase1 Size-exclusion chromatography Mice cerebral cortex 19066616
CARD NLRP1 Link Caspase2 Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 11076957
CARD NLRP1 Link Caspase2 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 12191486
CARD NLRP1 Link Caspase4 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 12191486
CARD NLRP1 Link Caspase5 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 12191486
CARD NLRP1 Link Caspase5 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 17418785
CARD NLRP1 Link NLRP1 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 11113115
CARD NLRP1 Link NLRP1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 21252346
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
CARD NLRP1 Link Apaf1 DAPI staining Apoptosis 11113115
CARD NLRP1 Link Caspase1 Luciferase assay Caspase1 activation (Inflammasome) 17349957
CARD NLRP1 Link Caspase1 ELISA IL-1b secretion increase 17418785
CARD NLRP1 Link Caspase1 Caspase cleaved assay Caspase1 activation (Inflammasome) 21252346
CARD NLRP1 Link Caspase5 Caspase cleaved assay Caspase5 activation (Inflammasome) 12191486
CARD NLRP1 Link Caspase5 ELISA IL-1b secretion increase 17418785
(Link: click this icon to show interactions only between the two corresponding DDs)