NLRP3 (PYD)

Full Name:
NACHT, LRR and PYD domains-containing protein 3
Alternative Names:
AII, AVP, FCU, MWS, FCAS, CIAS1, NALP3, C1orf7, CLR1.1, PYPAF1, AII/AVP, AGTAVPRL, FLJ95925, Cryopyrin
Function:
May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.
Death Domain Subfamily & Boundary Region:
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq Q96P20external db link DIP-41153Nexternal db link Q96P20external db link hsa:114548external db link
Mouse Seq Q8R4B8external db link Q8R4B8external db link mmu:216799external db link
Rat Seq D4A523external db link
Dog Seq E2QZH5external db link
Structure Species Residue Method Expression Purification Column Final condition Final concentration Reference
3QF2 Download Human 3-110 X-ray E.coli Gel filtration Superose 6 20mM sodium citrate(pH5.0), 150mM NaCl 8mg/ml 21880711
Disease Mutation in disease Reference
CINCA syndrome D303N, F309S 12032915
Familial cold autoinflammatory syndrome A439V, V198M, E627G 11687797
Familial cold autoinflammatory syndrome R260W 11992256
Familial cold autoinflammatory syndrome L353P 12522564
Muckle-wells syndrome A352V 11687797
Muckle-wells syndrome T348M, D303N, G569R, A439T 11992256
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
PYD NLRP3 Link ASC Co-immunoprecipitation Not specified 1-112 HEK293 THP-1 lysate 15030775
PYD NLRP3 Link ASC GST fusion protein pull-down Not specified Full length In vitro translation Not specified 1-91 E.coli 23066025
PYD NLRP3 Link ASC Two-hybrid Not specified 1-116 HEK293 Not specified 1-110 HEK293 11786556
PYD NLRP3 Link ASC Yeast two-hybrid Human 1-101 Yeast Human Full length Yeast 19337385
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
PYD NLRP3 Link ASC Co-Immunofluorescence staining HEK293 Not specified Full length Not specified Not specified 11786556
PYD NLRP3 Link ASC Co-Immunofluorescence staining HEK293 Not specified 1-739 Not specified Not specified 11786556
PYD NLRP3 Link ASC Co-Immunofluorescence staining HEK293 Not specified Full length Human Full length 16037825
PYD NLRP3 Link ASC Co-immunoprecipitation HEK293 Human 1-96 Human Not specified 12615073
PYD NLRP3 Link ASC Co-immunoprecipitation HEK293 Not specified 1-112 Not specified Not specified 15030775
PYD NLRP3 Link ASC Co-immunoprecipitation THP-1 15030775
PYD NLRP3 Link ASC Co-immunoprecipitation HEK293 Human 1-112 Not specified Not specified 15030775
PYD NLRP3 Link ASC Co-immunoprecipitation HEK293 Not specified 1-87 Human Not specified 19158675
PYD NLRP3 Link ASC Co-immunoprecipitation HMVEC-d 21575908
PYD NLRP3 Link ASC Co-immunoprecipitation HuH-7.5 21994322
PYD NLRP3 Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 22361007
PYD NLRP3 Link ASC Co-immunoprecipitation HEK293 Not specified Full length Not specified 1-91 23066025
PYD NLRP3 Link ASC Fluorescence resonance energy transfer HEK293 Not specified 1-87 Human Not specified 19158675
PYD NLRP3 Link NLRP3 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 17483456
PYD NLRP3 Link NLRP3 Size-exclusion chromatography HEK293 Not specified Full length Not specified Full length 17483456
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
PYD NLRP3 Link ASC Co-immunoprecipitation E13A, K21A, R41A, D48A, D51A (ASC) 23066025
PYD NLRP3 Link ASC GST fusion protein pull-down E13A, K21A, R41A, D48A, D51A (ASC) 23066025
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
PYD NLRP3 Link ASC Luciferase assay NF-kB activation (Inflammasome) 11786556
PYD NLRP3 Link ASC Caspase cleaved assay Caspase1 activation (Inflammasome) 16037825
PYD NLRP3 Link ASC Luciferase assay NF-kB activation (Inflammasome) 12615073
PYD NLRP3 Link ASC Caspase cleaved assay Caspase1 activation (Inflammasome) 15030775
(Link: click this icon to show interactions only between the two corresponding DDs)