NOD1 (CARD)

Full Name:
Nucleotide-binding oligomerization domain-containing protein 1
Alternative Names:
CARD4, NLRC1, CLR7.1
Function:
Enhances caspase-9-mediated apoptosis. Induces NF-kappa-B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappaB by Shigella effector proteins IpgB2 and OspB.
Death Domain Subfamily & Boundary Region:
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq Q9Y239external db link DIP-41064Nexternal db link Q9Y239external db link hsa:10392external db link Q9Y239external db link MINT-90728external db link
Mouse Seq Q8BHB0external db link Q8BHB0external db link mmu:107607external db link
Rat Seq D4ADT7external db link
Dog Seq E2R9L3external db link cfa:482382external db link
Zebra fish Seq F1QVM0external db link
Structure Species Residue Method Expression Purification Column Final condition Final concentration Reference
2B1W Download Human 15-138 NMR E.coli Gel filtration Superdex 75 25mM sodium phosphate(pH7.0), 100mM NaCl, 5mM DTT 1.4mM 17054981
2DBD Download Human NMR
2NSN Download Human 16-108 X-ray E.coli Gel filtration Superdex S75 20mM tris(pH8.0), 10mM NaCl, 5mM DTT) 20mg/ml 17173864
2NZ7 Download Human 1-106 X-ray E.coli Gel filtration Superdex S75 10mM sodium phosphate(pH6.0), 130mM NaCl, 1mM DTT 13mg/ml 18186648
No information
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
CARD NOD1 Link RIPK2 GST fusion protein pull-down Not specified 17-138 E.coli Not specified 435-528 E.coli 22470564
CARD NOD1 Link RIPK2 Yeast two-hybrid Human 1-145 Yeast Not specified Full length Yeast 10224040
CARD NOD1 Link RIPK2 Yeast two-hybrid Human 1-145 Yeast Not specified 384-540 Yeast 10224040
CARD NOD1 Link RIPK2 Yeast two-hybrid Human 1-110 Yeast Human 427-527 Yeast 19337385
CARD NOD1 Link RIPK2 Yeast two-hybrid Human 16-108 Yeast Human 427-527 Yeast 19337385
(Link: click this icon to show interactions only between the two corresponding DDs)
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In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD NOD1 Link CARD6 Co-immunoprecipitation HEK293 Not specified Not specified Human 1-311 12775719
CARD NOD1 Link Caspase1 Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 10329646
CARD NOD1 Link Caspase1 Co-immunoprecipitation HEK293 Not specified 1-158 Not specified 1-102 12459189
CARD NOD1 Link Caspase2 Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 10329646
CARD NOD1 Link Caspase4 Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 10329646
CARD NOD1 Link Caspase9 Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 10329646
CARD NOD1 Link NOD1 Co-immunoprecipitation HEK293 Human Full length Human Full length 10329646
CARD NOD1 Link NOD1 Co-immunoprecipitation HEK293 Not specified Full length Not specified 1-648 10880512
CARD NOD1 Link NOD1 Co-immunoprecipitation HEK293 Not specified 1-648 Not specified 1-648 10880512
CARD NOD1 Link RIPK2 Co-immunoprecipitation HEK293 Human Full length Not specified Full length 10224040
CARD NOD1 Link RIPK2 Co-immunoprecipitation HEK293 Human Full length Not specified 374-540 10329646
CARD NOD1 Link RIPK2 Co-immunoprecipitation HEK293 Human Full length Not specified Not specified 11463746
CARD NOD1 Link RIPK2 Co-immunoprecipitation HEK293 Not specified 1-158 Not specified 365-531 12459189
CARD NOD1 Link RIPK2 Co-immunoprecipitation HEK293 Human Full length Not specified Full length 17054981
(Link: click this icon to show interactions only between the two corresponding DDs)
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Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
CARD NOD1 Link Caspase9 Co-immunoprecipitation V41Q(NOD1) 10329646
CARD NOD1 Link NOD1 Co-immunoprecipitation V41Q(NOD1) 10880512
CARD NOD1 Link RIPK2 Co-immunoprecipitation V41Q(NOD1) 10329646
CARD NOD1 Link RIPK2 Co-immunoprecipitation E53K, D54K, E56K (NOD1) R444E, R483E, R488E (RIPK2) 17054981
CARD NOD1 Link RIPK2 GST fusion protein pull-down D461A, E472A, E475A, E492A (RIPK2) 22470564
(Link: click this icon to show interactions only between the two corresponding DDs)
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Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
CARD NOD1 Link CARD6 Luciferase assay NF-kB activation block 12775719
CARD NOD1 Link Caspase1 Immunoprecipitation Caspase1 oligomerization enhance 12459189
CARD NOD1 Link Caspase9 Caspase cleaved assay, β-galactosidase staining Caspase9 activation, Caspase9 induced Apoptosis 10329646
CARD NOD1 Link NOD1 Luciferase assay NF-kB activation(Nodosome) 10880512
CARD NOD1 Link RIPK2 Luciferase assay NF-kB activation 10329646
CARD NOD1 Link RIPK2 Reporter gene assay NF-kB activation (Nodosome) 11463746
(Link: click this icon to show interactions only between the two corresponding DDs)