NOD2 (CARD)

Full Name:
Nucleotide-binding oligomerization domain-containing protein 2
Alternative Names:
CD, ACUG, BLAU, IBD1, NLRC2, NOD2B, CARD15, CLR16.3, PSORAS1
Function:
Induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS).
Death Domain Subfamily & Boundary Region:
  • CARD : 26-122, 126-218 Seq
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq Q9HC29external db link DIP-41998Nexternal db link Q9HC29external db link hsa:64127external db link MINT-151071external db link
Mouse Seq Q8K3Z0external db link DIP-46115Nexternal db link Q8K3Z0external db link
Rat Seq D4A5W3external db link rno:291912external db link
Zebra fish Seq Q1AMZ9external db link Q1AMZ9external db link
Not solved yet
Disease Mutation in disease Reference
Blau syndrome L469E, R334Q, R334W 11528384
Crohn's disease R675W, G881R, 980fs 11385576
Crohn's disease 1007fs 11385577
Crohn's disease 12577202
Early onset sarcoidosis R334W, H496L, M513T, T605P, N670K, D382E, A612T 15459013
Psoriatic arthritis R702W, 1007fs, G908R 12879366
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
CARD NOD2 Link NOD2 In vitro purification protein assembly(Isothermal titration calorimety) Not specified 28-119 E.coli Not specified 120-218 E.coli 22470564
CARD NOD2 Link NOD2 Yeast two-hybrid Human 1-267 Yeast Human 1-267 Yeast 19337385
CARD NOD2 Link RIG-1 His Fusion protein pull-down Not specified 1-301 E.coli Not specified 1-205 E.coli 21690088
CARD NOD2 Link RIPK2 GST fusion protein pull-down Not specified 28-218 E.coli Not specified 435-528 E.coli 22470564
CARD NOD2 Link RIPK2 Yeast two-hybrid Human 1-267 Yeast Human 427-527 Yeast 19337385
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD NOD2 Link CARD12 Co-immunoprecipitation HEK293 Not specified Not specified Human Not specified 11472070
CARD NOD2 Link Caspase1 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 18511561
CARD NOD2 Link RIG-1 Co-Immunofluorescence staining HT29 21690088
CARD NOD2 Link RIG-1 Co-Immunofluorescence staining HEK293 Not specified Not specified Not specified Not specified 21690088
CARD NOD2 Link RIG-1 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 21690088
CARD NOD2 Link RIG-1 Co-immunoprecipitation HEK293 Not specified 1-301 Not specified Full length 21690088
CARD NOD2 Link RIG-1 Co-immunoprecipitation HEK293 Not specified 1-301 Not specified 1-225 21690088
CARD NOD2 Link RIG-1 Co-immunoprecipitation HEK293 Not specified Full length Not specified 1-225 21690088
CARD NOD2 Link RIPK2 Co-immunoprecipitation HEK293 Human Full length Not specified Full length 11087742
CARD NOD2 Link RIPK2 Co-immunoprecipitation HEK293 Human 1-744 Not specified Full length 11087742
CARD NOD2 Link RIPK2 Co-immunoprecipitation HEK293 Human 1-301 Not specified Full length 11087742
CARD NOD2 Link RIPK2 Co-immunoprecipitation HEK293 Human Full length Not specified 374-540 11087742
CARD NOD2 Link RIPK2 Co-immunoprecipitation HEK293 Mouse Not specified Not specified Not specified 15620648
CARD NOD2 Link RIPK2 Co-immunoprecipitation HEK293 Mouse Not specified Not specified Not specified 18775659
CARD NOD2 Link RIPK2 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Full length 19464198
CARD NOD2 Link RIPK2 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 22665475
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
CARD NOD2 Link NOD2 Isothermal titration calorimety 1:1 1μM 22470564
CARD NOD2 Link RIPK2 GST fusion protein pull-down R38A, R86A (NOD2) D461A, E472A, D473A, E475A, D492A (RIPK2) 22470564
CARD NOD2 Link RIPK2 Yeast two-hybrid E69K, D70K (Nod2) 19337385
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
CARD NOD2 Link Caspase1 Caspase cleaved assay Caspase1 activation 18511561
CARD NOD2 Link RIG-1 Reporter gene assay NF-kB activation block, IFN-b activation block 21690088
CARD NOD2 Link RIPK2 Luciferase assay NF-kB activation(Nodosome) 11087742
CARD NOD2 Link RIPK2 ELISA NF-kB activation (Nodosome) 18775659
(Link: click this icon to show interactions only between the two corresponding DDs)