PIDD (DD)

Full Name:
Leucine-rich repeat and death domain-containing protein
Alternative Names:
MGC16925, DKFZp434D229, LRDD
Function:
Promotes apoptosis downstream of the tumor suppressor as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis. Associates with NEMO/IKBKG and RIP1 and enhances sumoylation and ubiquitination of NEMO/IKBKG which is important for activation of the transcription factor NF-kappa-B. Associates with CASP2/caspase-2 and CRADD/RAIDD, and induces activation of CASP2 which an important regulator in apoptotic pathways.
Death Domain Subfamily & Boundary Region:
  • DD : 788-873 Seq
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq Q9HB75external db link DIP-34551Nexternal db link Q9HB75external db link hsa:55367external db link Q9HB75external db link MINT-1474528external db link
Mouse Seq Q9ERV7external db link Q9ERV7external db link mmu:57913external db link
Rat Seq D3ZZY5external db link DIP-48600Nexternal db link rno:293625external db link
Dog Seq E2RJ80external db link
Zebra fish Seq F1Q7P6external db link
Structure Species Residue Method Expression Purification Column Final condition Final concentration Reference
2OF5 Download Human 778-883 X-ray E.coli Gel filtration Superdex 200 12mg/ml 17289572
No information
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD PIDD Link RAIDD In vitro purification protein assembly(Native gel-electrophoresis) Human 777-883 E.coli Human 94-199 E.coli 20406701
DD PIDD Link RAIDD In vitro purification protein assembly(Native gel-electrophoresis) Human 777-883 E.coli Human Full length E.coli 20872280
DD PIDD Link RAIDD In vitro purification protein assembly(Size-exclusion chromatography) Not specified Full length HEK293 Not specified 101-199 HEK293 15073321
DD PIDD Link RAIDD In vitro purification protein assembly(Size-exclusion chromatography) Human 777-883 E.coli Human 94-199 E.coli 17289572
DD PIDD Link RAIDD In vitro purification protein assembly(Size-exclusion chromatography) Human 777-883 E.coli Human 94-199 E.coli 17329820
DD PIDD Link RAIDD In vitro purification protein assembly(Size-exclusion chromatography) Human 777-883 E.coli Human 94-199 E.coli 20406701
DD PIDD Link RAIDD In vitro purification protein assembly(Size-exclusion chromatography) Human 777-883 E.coli Human Full length E.coli 20872280
(Link: click this icon to show interactions only between the two corresponding DDs)
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In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD PIDD Link FADD Co-immunoprecipitation COS-7 Not specified 1-753 Not specified Not specified 10825539
DD PIDD Link FADD Co-immunoprecipitation HEK293 Not specified Not specified Not used 16360037
DD PIDD Link MADD Co-immunoprecipitation COS-7 Not specified 1-753 Not specified Not specified 10825539
DD PIDD Link RAIDD Co-Immunofluorescence staining HCT116 16652156
DD PIDD Link RAIDD Co-immunoprecipitation HEK293 Not specified Full length Not specified 101-199 15073321
DD PIDD Link RAIDD Co-immunoprecipitation Jurkat Not specified Not specified Not used 16360037
DD PIDD Link RAIDD Co-immunoprecipitation HCT116 16652156
DD PIDD Link RAIDD Co-immunoprecipitation HEK293 Not specified 588-910 Not specified Not specified 17159900
DD PIDD Link RAIDD Co-immunoprecipitation HEK293 Not specified 777-883 Human 94-199 17289572
DD PIDD Link RAIDD Co-immunoprecipitation Hippocampal CA1 subregion 18845684
DD PIDD Link RAIDD Co-immunoprecipitation HEK293 Not specified Not specified Not used 20966961
DD PIDD Link RAIDD Size-exclusion chromatography HEK293 Not specified Not specified Not used 20966961
DD PIDD Link RIP Co-immunoprecipitation HEK293 Not specified Not specified Not used 16360037
DD PIDD Link RIP Co-immunoprecipitation HEK293 Not specified 446-910 Not specified Not specified 17159900
(Link: click this icon to show interactions only between the two corresponding DDs)
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Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DD PIDD Link RAIDD Gel filtration 5:7 2OF5 17289572
DD PIDD Link RAIDD Gel filtration 7:5 2OF5 17289572
DD PIDD Link RAIDD Gel filtration 5:7 20872280
DD PIDD Link RAIDD Gel filtration 7:5 20872280
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DD PIDD Link RAIDD Trypan blue staining Apoptosis (PIDDosome) 15073321
DD PIDD Link RAIDD Caspase cleaved assay Caspase2 activation (PIDDosome) 17159900
DD PIDD Link RAIDD TUNEL assay Apoptosis (PIDDosome) 18845684
DD PIDD Link RIP NEMO sumoylation, Ubquitination assay NF-kB activation 16360037
DD PIDD Link RIP Kinase assay NF-kB activation 17159900
(Link: click this icon to show interactions only between the two corresponding DDs)