POP1 (PYD)

Full Name:
Pyrin domain-containing protein 1
Alternative Names:
ASC2, PYC1, PYDC1
Function:
Associates with apoptosis-associated specklike protein containing a CARD domain (ASC) and modulates its ability to collaborate with pyrin and cryopyrin in NF-kappa-B and pro-caspase-1 activation. Suppresses kinase activity of NF-kappa-B inhibitor kinase (IKK) complex, expression of NF-kappa-B inducible genes and inhibits NF-kappa-B activation by cytokines and LPS.
Death Domain Subfamily & Boundary Region:
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq Q8WXC3external db link Q8WXC3external db link hsa:260434external db link
Structure Species Residue Method Expression Purification Column Final condition Final concentration Reference
2HM2 Download Human 1-89 NMR E.coli Affinity chromatography 10mM sodium phosphate(pH7.3), 140mM NaCl 1mM 16905547
No information
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
PYD POP1 Link ASC GST fusion protein pull-down Human Not specified In vitro translation Not specified 1-100 E.coli 12656673
PYD POP1 Link ASC GST fusion protein pull-down Not specified 1-89 In vitro translation Not specified 1-91 E.coli 23066025
PYD POP1 Link ASC In vitro purification protein assembly(Surface plasmon resonance) Not specified 1-89 E.coli Not specified 1-96 E.coli 18362139
(Link: click this icon to show interactions only between the two corresponding DDs)
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In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
PYD POP1 Link ASC Co-Immunofluorescence staining COS-7 Not specified Not specified Not specified Not specified 12656673
PYD POP1 Link ASC Co-immunoprecipitation HEK293 Human Not specified Not specified 1-100 12656673
PYD POP1 Link ASC Co-immunoprecipitation HEK293 Human Not specified Human Not specified 17178784
PYD POP1 Link ASC Co-immunoprecipitation HEK293 Not specified 1-89 Not specified 1-91 23066025
(Link: click this icon to show interactions only between the two corresponding DDs)
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Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
PYD POP1 Link ASC Co-immunoprecipitation E13A, K21A, R41A, D48A, D51A (ASC) 23066025
PYD POP1 Link ASC GST fusion protein pull-down R3A, D10A, E13A, K21A, R38A, R41A, D48A, D51A, D54A, Q84A (ASC) 23066025
PYD POP1 Link ASC Surface plasmon resonance 1:1 4.8μM D6A and L25A, E13A and L25A, K21A, Y36A, D48A and L25A, D54A and L25A (ASC) 18362139
PYD POP1 Link ASC Surface plasmon resonance 1:1 4.8μM D6A and L25A, E13A and L25A, K21A, Y36A, D48A and L25A, D54A and L25A (ASC) 18362139
(Link: click this icon to show interactions only between the two corresponding DDs)
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Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
PYD POP1 Link ASC Luciferase assay NF-kB activation block 12656673
(Link: click this icon to show interactions only between the two corresponding DDs)