RAIDD (CARD)

Full Name:
Death domain-containing protein CRADD
Alternative Names:
MGC9163, CRADD
Function:
Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex.
Death Domain Subfamily & Boundary Region:
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq P78560external db link DIP-34550Nexternal db link P78560external db link hsa:8738external db link P78560external db link MINT-1520976external db link
Mouse Seq O88843external db link O88843external db link mmu:12905external db link
Rat Seq D3ZB14external db link DIP-48601Nexternal db link rno:314756external db link
Dog Seq E2R247external db link
Structure Species Residue Method Expression Purification Column Final condition Final concentration Reference
3CRD Download Human 1-100 NMR E.coli Gel filtration Sephadex G-50 20mM tris(pH8.0), 5mM NaCl 1mM 9695946
No information
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
CARD RAIDD Link Bcl-10 Two-hybrid Not specified Not specified HEK293 Not specified Not specified HEK293 22323537
CARD RAIDD Link Caspase2 GST fusion protein pull-down Human Not specified E.coli Not specified Not specified In vitro translation 9044836
CARD RAIDD Link Caspase2 His fusion protein pull-down Not specified Not specified E.coli Human Full length In vitro translation 8985253
CARD RAIDD Link Caspase2 His fusion protein pull-down Not specified 1-117 E.coli Human Full length In vitro translation 8985253
CARD RAIDD Link Caspase2 His fusion protein pull-down Not specified Not specified E.coli Human 1-140 In vitro translation 8985253
CARD RAIDD Link Caspase2 In vitro purification protein assembly(Native gel-electrophoresis) Human Full length E.coli Human 31-121 E.coli 20872280
CARD RAIDD Link Caspase2 In vitro purification protein assembly(Size-exclusion chromatography) Not specified Full length HEK293 Not specified 1-104 HEK293 15073321
CARD RAIDD Link Caspase2 In vitro purification protein assembly(Size-exclusion chromatography) Human Full length E.coli Human 31-121 E.coli 20872280
CARD RAIDD Link Caspase2 Yeast two-hybrid Not specified Not specified Yeast Human Not specified Yeast 11156409
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD RAIDD Link Bcl-10 Co-immunoprecipitation HEK293 Not used Not specified Not specified 22323537
CARD RAIDD Link Caspase2 Co-Immunofluorescence staining HEK293 Human Full length Mouse Full length 10713730
CARD RAIDD Link Caspase2 Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 8985253
CARD RAIDD Link Caspase2 Co-immunoprecipitation HEK293 Human Full length Mouse Full length 10713730
CARD RAIDD Link Caspase2 Co-immunoprecipitation Namalwa Not specified Not specified Human Not specified 11156409
CARD RAIDD Link Caspase2 Co-immunoprecipitation Mouse brain cell 11978827
CARD RAIDD Link Caspase2 Co-immunoprecipitation HEK293 Not specified Full length Not specified 1-104 15073321
CARD RAIDD Link Caspase2 Co-immunoprecipitation HCL116 16652156
CARD RAIDD Link Caspase2 Co-immunoprecipitation Hippocampal CA1 subregion 18845684
CARD RAIDD Link Caspase2 Co-immunoprecipitation HEK293 Not specified Not specified Not specified 1-122 19782032
CARD RAIDD Link Caspase2 Co-immunoprecipitation rat hippocampal neuron 22515271
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
CARD RAIDD Link Bcl-10 Immunoprecipitation Bcl10-CARMA1 complex inhibition 22323537
CARD RAIDD Link Caspase2 β-galactosidase staining Apoptosis (PIDDosome) 8985253
CARD RAIDD Link Caspase2 Trypan blue staining Apoptosis (PIDDosome) 15073321
CARD RAIDD Link Caspase2 TUNEL assay Apoptosis (PIDDosome) 18845684
(Link: click this icon to show interactions only between the two corresponding DDs)