RAIDD (DD)

Full Name:
Death domain-containing protein CRADD
Alternative Names:
MGC9163, CRADD
Function:
Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex.
Death Domain Subfamily & Boundary Region:
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq P78560external db link DIP-34550Nexternal db link P78560external db link hsa:8738external db link P78560external db link MINT-1520976external db link
Mouse Seq O88843external db link O88843external db link mmu:12905external db link
Rat Seq D3ZB14external db link DIP-48601Nexternal db link
Dog Seq E2R247external db link
Zebra fish Seq Q5XJP2external db link Q5XJP2external db link dre:450046external db link
Structure Species Residue Method Expression Purification Column Final condition Final concentration Reference
2O71 Download Human 109-199 X-ray E.coli Gel filtration 6mg/ml 16434054
2OF5 Download Human 94-199 X-ray E.coli Gel filtration Superdex 200 12mg/ml 17289572
No information
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD RAIDD Link PIDD In vitro purification protein assembly(Native gel-electrophoresis) Human 94-199 E.coli Human 777-883 E.coli 20406701
DD RAIDD Link PIDD In vitro purification protein assembly(Native gel-electrophoresis) Human Full length E.coli Human 777-883 E.coli 20872280
DD RAIDD Link PIDD In vitro purification protein assembly(Size-exclusion chromatography) Not specified 101-199 HEK293 Not specified Full length HEK293 15073321
DD RAIDD Link PIDD In vitro purification protein assembly(Size-exclusion chromatography) Human 94-199 E.coli Human 777-883 E.coli 17289572
DD RAIDD Link PIDD In vitro purification protein assembly(Size-exclusion chromatography) Human 94-199 E.coli Human 777-883 E.coli 17329820
DD RAIDD Link PIDD In vitro purification protein assembly(Size-exclusion chromatography) Human 94-199 E.coli Human 777-883 E.coli 20406701
DD RAIDD Link PIDD In vitro purification protein assembly(Size-exclusion chromatography) Human Full length E.coli Human 777-883 E.coli 20872280
DD RAIDD Link RIP GST fusion protein pull-down Human Not specified E.coli Human Not specified In vitro translation 9044836
(Link: click this icon to show interactions only between the two corresponding DDs)
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In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD RAIDD Link PIDD Co-Immunofluorescence staining HCT116 16652156
DD RAIDD Link PIDD Co-immunoprecipitation HEK293 Not specified 101-199 Not specified Full length 15073321
DD RAIDD Link PIDD Co-immunoprecipitation Jurkat Not used Not specified Not specified 16360037
DD RAIDD Link PIDD Co-immunoprecipitation HCT116 16652156
DD RAIDD Link PIDD Co-immunoprecipitation HEK293 Not specified Not specified Not specified 588-910 17159900
DD RAIDD Link PIDD Co-immunoprecipitation HEK293 Human 94-199 Not specified 777-883 17289572
DD RAIDD Link PIDD Co-immunoprecipitation Hippocampal CA1 subregion 18845684
DD RAIDD Link PIDD Co-immunoprecipitation HEK293 Not used Not specified Not specified 20966961
DD RAIDD Link PIDD Size-exclusion chromatography HEK293 Not used Not specified Not specified 20966961
DD RAIDD Link RIP Co-immunoprecipitation Mouse brain cell 11978827
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DD RAIDD Link PIDD Gel filtration 5:7 2OF5 17289572
DD RAIDD Link PIDD Gel filtration 7:5 2OF5 17289572
DD RAIDD Link PIDD Gel filtration 5:7 20872280
DD RAIDD Link PIDD Gel filtration 7:5 20872280
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DD RAIDD Link PIDD Trypan blue staining Apoptosis (PIDDosome) 15073321
DD RAIDD Link PIDD Caspase cleaved assay Caspase2 activation (PIDDosome) 17159900
DD RAIDD Link PIDD TUNEL assay Apoptosis (PIDDosome) 18845684
(Link: click this icon to show interactions only between the two corresponding DDs)