RIG-1 (CARD)

Full Name:
Probable ATP-dependent RNA helicase DDX58
Alternative Names:
DDX58, FLJ13599, DKFZp434J1111, DKFZp686N19181
Function:
Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV (By similarity).
Death Domain Subfamily & Boundary Region:
  • CARD : 1-87, 92-172 Seq
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq O95786external db link DIP-35444Nexternal db link O95786external db link hsa:23586external db link O95786external db link MINT-2799116external db link
Mouse Seq Q6Q899external db link Q6Q899external db link mmu:230073external db link
Dog Seq E2RMV6external db link
Structure Species Residue Method Expression Purification Column Final condition Final concentration Reference
4A2W Download Duck 1-933 X-ray BTI-TN-5B1-4 Gel filtration Superdex 200 20mM HEPES(pH 7.5), 150mM NaCl, 2.5mM MgCl2, 5mM betamercaptoethanol 7mg/ml 22000019
No information
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
CARD RIG-1 Link NOD2 His Fusion protein pull-down Not specified 1-205 E.coli Not specified 1-301 E.coli 21690088
(Link: click this icon to show interactions only between the two corresponding DDs)
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In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD RIG-1 Link Caspase12 Co-Immunofluorescence staining Mouse embryonic fibroblast Human Not specified Not specified Not specified 20818395
CARD RIG-1 Link Caspase12 GST fusion protein pull-down Mouse embryonic fibroblast Human Not specified Not specified Not specified 20818395
CARD RIG-1 Link MAVS Co-immunoprecipitation HEK293 Human 1-604 Human Not specified 16127453
CARD RIG-1 Link MAVS Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 16177806
CARD RIG-1 Link MAVS Co-immunoprecipitation HEK293 Not specified Not specified Human Not specified 17020950
CARD RIG-1 Link MAVS Co-immunoprecipitation HEK293 Not specified Not specified Not used 21419663
CARD RIG-1 Link MAVS Fluorescence resonance energy transfer Huh 7 Not specified Not specified Not specified Not specified 19036819
CARD RIG-1 Link MDA5 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 22328336
CARD RIG-1 Link NOD2 Co-Immunofluorescence staining HT29 21690088
CARD RIG-1 Link NOD2 Co-Immunofluorescence staining HEK293 Not specified Not specified Not specified Not specified 21690088
CARD RIG-1 Link NOD2 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 21690088
CARD RIG-1 Link NOD2 Co-immunoprecipitation HEK293 Not specified Full length Not specified 1-301 21690088
CARD RIG-1 Link NOD2 Co-immunoprecipitation HEK293 Not specified 1-225 Not specified 1-301 21690088
CARD RIG-1 Link NOD2 Co-immunoprecipitation HEK293 Not specified 1-225 Not specified Full length 21690088
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
CARD RIG-1 Link MAVS ELISA Type 1 interferon induced 16127453
CARD RIG-1 Link MAVS Luciferase assay IFN-b induced 16177806
CARD RIG-1 Link NOD2 Reporter gene assay NF-kB activation block, IFN-b activation block 21690088
(Link: click this icon to show interactions only between the two corresponding DDs)