RIP (DD)

Full Name:
Receptor-interacting serine/threonine-protein kinase 1
Alternative Names:
RIP1, FLJ39204, RIPK1
Function:
Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains.
Death Domain Subfamily & Boundary Region:
  • DD : 583-669 Seq
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq Q13546external db link DIP-433Nexternal db link Q13546external db link hsa:8737external db link Q13546external db link MINT-128219external db link
Mouse Seq Q60855external db link DIP-34962Nexternal db link Q60855external db link mmu:19766external db link Q60855external db link MINT-144784external db link
Rat Seq D3ZYL0external db link rno:306886external db link
Dog Seq F1PQX4external db link
Not solved yet
No information
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD RIP Link FADD Yeast two-hybrid Not specified 261-372 Yeast Not specified 92-208 Yeast 8642271
DD RIP Link FADD Yeast two-hybrid Human 580-671 Yeast Human Full length Yeast 10755617
DD RIP Link FADD Yeast two-hybrid Human Full length Yeast Human 80-208 Yeast 11464214
DD RIP Link Fas Yeast two-hybrid Human Full length Yeast Human 192-329 Yeast 7538908
DD RIP Link Fas Yeast two-hybrid Not specified 261-372 Yeast Not specified 210-319 Yeast 8642271
DD RIP Link RAIDD GST fusion protein pull-down Human Not specified In vitro translation Human Not specified E.coli 9044836
DD RIP Link RIP Yeast two-hybrid Not specified 261-372 Yeast Not specified 261-372 Yeast 8642271
DD RIP Link RIP Yeast two-hybrid Human 580-671 Yeast Human Full length Yeast 10755617
DD RIP Link TNFR1 GST fusion protein pull-down Jurkat lysate Not specified 237-455 E.coli 12721308
DD RIP Link TNFR1 Yeast two-hybrid Not specified 261-372 Yeast Not specified 326-426 Yeast 8642271
DD RIP Link TRADD Yeast two-hybrid Not specified 261-372 Yeast Not specified 195-312 Yeast 8642271
DD RIP Link TRADD Yeast two-hybrid Human 580-671 Yeast Human Full length Yeast 10755617
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD RIP Link DR4 Co-immunoprecipitation HEK293 Human 559-671 Human 1-351 10958661
DD RIP Link DR4 His fusion protein pull-down HEK293 Human Not specified Human 106-468 9430227
DD RIP Link DR5 Co-immunoprecipitation HEK293 Human Not specified Human 51-411 9430227
DD RIP Link FADD Co-Immunofluorescence staining HEK293 Human Not specified Not specified Not specified 11002422
DD RIP Link FADD Co-immunoprecipitation HeLa Not specified 261-372 Not specified 19-753 8642271
DD RIP Link PIDD Co-immunoprecipitation HEK293 Not used Not specified Not specified 16360037
DD RIP Link PIDD Co-immunoprecipitation HEK293 Not specified Not specified Not specified 446-910 17159900
DD RIP Link RAIDD Co-immunoprecipitation Mouse brain cell 11978827
DD RIP Link TNFR1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 8612133
DD RIP Link TNFR1 Co-immunoprecipitation HEK293 Not specified Full length Not specified Not specified 8612133
DD RIP Link TNFR1 Co-immunoprecipitation HEK293 Not specified 240-671 Not specified Not specified 8612133
DD RIP Link TNFR1 Co-immunoprecipitation HEK293 Not specified 559-671 Not specified Not specified 8612133
DD RIP Link TNFR1 Co-immunoprecipitation HEK293 Not specified 37-205 Not specified Not specified 9082980
DD RIP Link TNFR1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified 37-205 9082980
DD RIP Link TNFR1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 9714541
DD RIP Link TNFR1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 10358032
DD RIP Link TNFR1 Co-immunoprecipitation HEK293 Human Not specified Not used 10755617
DD RIP Link TNFR1 Co-immunoprecipitation Fibroblast 10795740
DD RIP Link TNFR1 Co-immunoprecipitation Jurkat Human Not specified Not specified Not specified 11002422
DD RIP Link TNFR1 Co-immunoprecipitation U937 11734559
DD RIP Link TNFR1 Co-immunoprecipitation Jurkat 12167698
DD RIP Link TNFR1 Co-immunoprecipitation Jurkat 16611992
DD RIP Link TNFR1 Co-immunoprecipitation Mice peritoneal macrophage lysate 18641653
DD RIP Link TNFR1 Co-immunoprecipitation HT1080 18939944
DD RIP Link TNFR1 Co-immunoprecipitation Jurkat 19524513
DD RIP Link TNFR1 Co-immunoprecipitation H2009 20547836
DD RIP Link TRADD Co-immunoprecipitation HEK293 Not specified Full length Not specified Not specified 8612133
DD RIP Link TRADD Co-immunoprecipitation HEK293 Not specified 240-671 Not specified Not specified 8612133
DD RIP Link TRADD Co-immunoprecipitation HEK293 Not specified 559-671 Not specified Not specified 8612133
DD RIP Link TRADD Co-immunoprecipitation HeLa Not specified 261-372 Not specified 195-312 8642271
DD RIP Link TRADD Co-immunoprecipitation HeLa 10521396
DD RIP Link TRADD Co-immunoprecipitation HEK293 Not used Not specified Not specified 10521396
DD RIP Link TRADD Co-immunoprecipitation Rat brain hippocampus 12786973
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DD RIP Link DR4 IKK assay, JNK assay IkB activation, JNK activation 10958661
DD RIP Link FADD Propidium iodide staining Apoptosis (DISC) 11002422
DD RIP Link PIDD NEMO sumoylation, Ubquitination assay NF-kB activation 16360037
DD RIP Link PIDD Kinase assay NF-kB activation 17159900
DD RIP Link TNFR1 Data not shown Apoptosis (DISC) 8612133
DD RIP Link TNFR1 IKK assay NF-kB activation (DISC) 10795740
DD RIP Link TNFR1 Propidium iodide staining Apoptosis (DISC) 11002422
DD RIP Link TNFR1 Propidium iodide staining Apoptosis (DISC) NF-kB activation (DISC) 16611992
(Link: click this icon to show interactions only between the two corresponding DDs)