RIPK2 (CARD)

Full Name:
Receptor-interacting serine/threonine-protein kinase 2
Alternative Names:
CCK, RICK, RIP2, CARD3, GIG30, CARDIAK
Function:
Activates pro-caspase-1 and pro-caspase-8. Potentiates CASP8-mediated apoptosis. Activates NF-kappa-B.
Death Domain Subfamily & Boundary Region:
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq O43353external db link DIP-27518Nexternal db link O43353external db link hsa:8767external db link O43353external db link MINT-90752external db link
Mouse Seq P58801external db link P58801external db link mmu:192656external db link
Rat Seq Q3B7U0external db link Q3B7U0external db link
Dog Seq E2QVN3external db link cfa:477936external db link
Zebra fish Seq Q4V958external db link Q4V958external db link dre:373874external db link
Not solved yet
No information
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
CARD RIPK2 Link Caspase1 GST fusion protein pull-down Not specified 454-540 E.coli Not specified 1-92 In vitro translation 11051551
CARD RIPK2 Link CIAP1 GST fusion protein pull-down Not specified Full length In vitro translation Not specified Not specified E.coli 19464198
CARD RIPK2 Link CIAP1 GST fusion protein pull-down Human Not specified In vitro translation Not specified Not specified E.coli 21931591
CARD RIPK2 Link CIAP2 GST fusion protein pull-down Not specified Full length In vitro translation Not specified Not specified E.coli 19464198
CARD RIPK2 Link CIAP2 GST fusion protein pull-down Human Not specified In vitro translation Not specified Not specified E.coli 21931591
CARD RIPK2 Link NOD1 GST fusion protein pull-down Not specified 435-528 E.coli Not specified 17-138 E.coli 22470564
CARD RIPK2 Link NOD1 Yeast two-hybrid Not specified Full length Yeast Human 1-145 Yeast 10224040
CARD RIPK2 Link NOD1 Yeast two-hybrid Not specified 384-540 Yeast Human 1-145 Yeast 10224040
CARD RIPK2 Link NOD1 Yeast two-hybrid Human 427-527 Yeast Human 1-110 Yeast 19337385
CARD RIPK2 Link NOD1 Yeast two-hybrid Human 427-527 Yeast Human 16-108 Yeast 19337385
CARD RIPK2 Link NOD2 GST fusion protein pull-down Not specified 435-528 E.coli Not specified 28-218 E.coli 22470564
CARD RIPK2 Link NOD2 Yeast two-hybrid Human 427-527 Yeast Human 1-267 Yeast 19337385
CARD RIPK2 Link RIPK2 Yeast two-hybrid Human 427-527 Yeast Human 427-527 Yeast 19337385
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD RIPK2 Link ASC Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 14634131
CARD RIPK2 Link Bcl-10 Co-immunoprecipitation HEK293 Not specified Full length Human Full length 14638696
CARD RIPK2 Link Bcl-10 Co-immunoprecipitation HEK293 Human Full length Not specified Full length 14638696
CARD RIPK2 Link CARD16 Co-immunoprecipitation HEK293 Not specified Full length Human Not specified 11432859
CARD RIPK2 Link CARD16 Co-immunoprecipitation HEK293 Not specified Not specified Human 1-106 11536016
CARD RIPK2 Link CARD18 Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 11536016
CARD RIPK2 Link CARD6 Co-immunoprecipitation HEK293 Not specified Not specified Human 1-311 12775719
CARD RIPK2 Link CARD9 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 17486093
CARD RIPK2 Link Caspase1 Co-immunoprecipitation HEK293 Human 2-92 Human 2-540 9705938
CARD RIPK2 Link Caspase1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Full length 11051551
CARD RIPK2 Link Caspase1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 11536016
CARD RIPK2 Link Caspase1 Co-immunoprecipitation HEK293 Murine Not specified Human 2-540 15039421
CARD RIPK2 Link Caspase1 Co-immunoprecipitation HEK293 Murine Not specified Human 431-540 15039421
CARD RIPK2 Link Caspase1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 16585594
CARD RIPK2 Link Caspase1 Co-immunoprecipitation THP-1 16585594
CARD RIPK2 Link Caspase1 Co-immunoprecipitation HEK239 Not specified Not specified Human Not specified 21862576
CARD RIPK2 Link CIAP1 Co-immunoprecipitation HEK293 Human Not specified Not specified Not specified 9642260
CARD RIPK2 Link CIAP1 Co-immunoprecipitation HEK293 Human 2-540 Not specified Not specified 9705938
CARD RIPK2 Link CIAP1 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 19464198
CARD RIPK2 Link CIAP1 Co-immunoprecipitation HEK239 Human Not specified Not specified Not specified 21931591
CARD RIPK2 Link CIAP2 Co-immunoprecipitation HEK293 Human 2-540 Not specified Not specified 9705938
CARD RIPK2 Link CIAP2 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 19464198
CARD RIPK2 Link CIAP2 Co-immunoprecipitation HEK239 Human Not specified Not specified Not specified 21931591
CARD RIPK2 Link NOD1 Co-immunoprecipitation HEK293 Not specified Full length Human Full length 10224040
CARD RIPK2 Link NOD1 Co-immunoprecipitation HEK293 Not specified 374-540 Human Full length 10329646
CARD RIPK2 Link NOD1 Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 11463746
CARD RIPK2 Link NOD1 Co-immunoprecipitation HEK293 Not specified 365-531 Not specified 1-158 12459189
CARD RIPK2 Link NOD1 Co-immunoprecipitation HEK293 Not specified Full length Human Full length 17054981
CARD RIPK2 Link NOD2 Co-immunoprecipitation HEK293 Not specified Full length Human Full length 11087742
CARD RIPK2 Link NOD2 Co-immunoprecipitation HEK293 Not specified Full length Human 1-744 11087742
CARD RIPK2 Link NOD2 Co-immunoprecipitation HEK293 Not specified Full length Human 1-301 11087742
CARD RIPK2 Link NOD2 Co-immunoprecipitation HEK293 Not specified 374-540 Human Full length 11087742
CARD RIPK2 Link NOD2 Co-immunoprecipitation HEK293 Not specified Not specified Mouse Not specified 15620648
CARD RIPK2 Link NOD2 Co-immunoprecipitation HEK293 Not specified Not specified Mouse Not specified 18775659
CARD RIPK2 Link NOD2 Co-immunoprecipitation HEK293 Not specified Full length Not specified Not specified 19464198
CARD RIPK2 Link NOD2 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 22665475
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
CARD RIPK2 Link ASC Co-immunoprecipitation L122Q(ASC) 14634131
CARD RIPK2 Link Caspase1 Co-immunoprecipitation D27G, R45D (Caspase1) 21862576
CARD RIPK2 Link NOD1 Co-immunoprecipitation V41Q(NOD1) 10329646
CARD RIPK2 Link NOD1 Co-immunoprecipitation E53K, D54K, E56K (NOD1) R444E, R483E, R488E (RIPK2) 17054981
CARD RIPK2 Link NOD1 GST fusion protein pull-down D461A, E472A, E475A, E492A (RIPK2) 22470564
CARD RIPK2 Link NOD2 GST fusion protein pull-down R38A, R86A (NOD2) D461A, E472A, D473A, E475A, D492A (RIPK2) 22470564
CARD RIPK2 Link NOD2 Yeast two-hybrid E69K, D70K (Nod2) 19337385
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
CARD RIPK2 Link ASC Immunoprecipitation Pro-caspase1-RIPK2 complex inhibition 14634131
CARD RIPK2 Link Bcl-10 Luciferase assay NF-kB activation 14638696
CARD RIPK2 Link CARD16 Immunoprecipitation Caspase1-RIPK2 complex interfere 11536016
CARD RIPK2 Link CARD6 Luciferase assay NF-kB activation block 12775719
CARD RIPK2 Link CARD9 Western blotting Bcl-10 phosphorylation increase 17486093
CARD RIPK2 Link Caspase1 Caspase cleaved assay Pro-caspase1 activation 9705938
CARD RIPK2 Link Caspase1 Caspase cleaved assay Caspase1 activation 11051551
CARD RIPK2 Link Caspase1 Reporter gene assay NF-kB activation 15039421
CARD RIPK2 Link Caspase1 Luciferase assay NF-kB activation 16585594
CARD RIPK2 Link CIAP1 β-galactosidase staining Apoptosis inhibition 9642260
CARD RIPK2 Link NOD1 Luciferase assay NF-kB activation 10329646
CARD RIPK2 Link NOD1 Reporter gene assay NF-kB activation (Nodosome) 11463746
CARD RIPK2 Link NOD2 Luciferase assay NF-kB activation(Nodosome) 11087742
CARD RIPK2 Link NOD2 ELISA NF-kB activation (Nodosome) 18775659
(Link: click this icon to show interactions only between the two corresponding DDs)