TNFR1 (DD)

Full Name:
Tumor necrosis factor receptor superfamily member 1A
Alternative Names:
FPF, p55, p60, TBP1, TNF-R, TNFAR, p55-R, CD120a, TNFR55, TNFR60, TNF-R-I, TNF-R55, MGC19588, TNFRSF1A
Function:
Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
Death Domain Subfamily & Boundary Region:
  • DD : 356-441 Seq
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq P19438external db link DIP-407Nexternal db link P19438external db link hsa:7132external db link P19438external db link MINT-135026external db link
Mouse Seq P25118external db link DIP-34532Nexternal db link P25118external db link mmu:21937external db link P25118external db link MINT-2631249external db link
Rat Seq P22934external db link P22934external db link rno:25625external db link P22934external db link
Zebra fish Seq Q6NUU6external db link Q6NUU6external db link dre:406471external db link
Structure Species Residue Method Expression Purification Column Final condition Final concentration Reference
1ICH Download Human 316-426 NMR E.coli Gel filtration G3000SW 10mM sodium acetate(pH4.0), 10mM DTT 6mg/ml 11453696
Disease Mutation in disease Reference
Tumor necrosis factor receptor-associated periodic syndrome (TRAPS) 9529351
Tumor necrosis factor receptor-associated periodic syndrome (TRAPS) C30R, C33Y, T50M, C52F, C88R 10199409
Tumor necrosis factor receptor-associated periodic syndrome (TRAPS) C30S 10902757
Tumor necrosis factor receptor-associated periodic syndrome (TRAPS) R92P 11175303
Tumor necrosis factor receptor-associated periodic syndrome (TRAPS) H22Y, C30S, C33G, P46L, S86P, R92Q 11443543
Tumor necrosis factor receptor-associated periodic syndrome (TRAPS) C70S 13130484
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD TNFR1 Link DR3 Yeast two-hybrid Human Not specified Yeast Not specified Not specified Yeast 8934525
DD TNFR1 Link FADD Yeast two-hybrid Not specified 326-426 Yeast Not specified 92-208 Yeast 8642271
DD TNFR1 Link MADD GST fusion protein pull-down Not specified 326-413 Not specified Not specified 982-1588 Not specified 9115275
DD TNFR1 Link MADD Yeast two-hybrid Not specified 326-413 Yeast Not specified 982-1588 Yeast 9115275
DD TNFR1 Link MADD Yeast two-hybrid Not specified 326-413 Yeast Not specified 1281-1356 Yeast 9115275
DD TNFR1 Link RIP GST fusion protein pull-down Not specified 237-455 E.coli Jurkat lysate 12721308
DD TNFR1 Link RIP Yeast two-hybrid Not specified 326-426 Yeast Not specified 261-372 Yeast 8642271
DD TNFR1 Link TNFR1 GST fusion protein pull-down Human 206-426 Not specified Human 206-426 Not specified 7529234
DD TNFR1 Link TNFR1 GST fusion protein pull-down Not specified 326-426 E.coli Not specified 214-455 E.coli 11231290
DD TNFR1 Link TNFR1 Yeast two-hybrid Human 206-426 Yeast Human 206-426 Yeast 7529234
DD TNFR1 Link TNFR1 Yeast two-hybrid Human 206-426 Yeast Human 328-426 Yeast 7529234
DD TNFR1 Link TNFR1 Yeast two-hybrid Not specified 204-426 Yeast Not specified 204-426 Yeast 8077196
DD TNFR1 Link TNFR1 Yeast two-hybrid Not specified 204-426 Yeast Not specified 329-426 Yeast 8077196
DD TNFR1 Link TNFR1 Yeast two-hybrid Not specified 326-426 Yeast Not specified 326-426 Yeast 8642271
DD TNFR1 Link TRADD GST fusion protein pull-down Human Not specified Not specified Human Full length In vitro translation 7758105
DD TNFR1 Link TRADD GST fusion protein pull-down Human Not specified Not specified Human 1-305 In vitro translation 7758105
DD TNFR1 Link TRADD GST fusion protein pull-down Human Not specified Not specified Human 195-312 In vitro translation 7758105
DD TNFR1 Link TRADD GST fusion protein pull-down Human 214-426 Not specified Human 196-312 In vitro translation 8621670
DD TNFR1 Link TRADD GST fusion protein pull-down Not specified Not specified E.coli Not specified Not specified In vitro translation 8875942
DD TNFR1 Link TRADD GST fusion protein pull-down Not specified 326-426 E.coli Not specified Not specified E.coli 11231290
DD TNFR1 Link TRADD GST fusion protein pull-down Not specified 237-455 E.coli Jurkat lysate 12721308
DD TNFR1 Link TRADD GST fusion protein pull-down Not specified 195-312 E.coli Not specified 243-455 In vitro translation 16006552
DD TNFR1 Link TRADD Yeast two-hybrid Human 214-426 Yeast Human Full length Yeast 7758105
DD TNFR1 Link TRADD Yeast two-hybrid Not specified 326-426 Yeast Not specified 195-312 Yeast 8642271
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD TNFR1 Link DAPK1 Co-Immunofluorescence staining Rat brain cell 12911633
DD TNFR1 Link DAPK1 Co-immunoprecipitation Rat brain cell 12911633
DD TNFR1 Link FADD Rat primary hepatocyte 21741934
DD TNFR1 Link FADD Co-immunoprecipitation HEK293 Not specified Not specified Not specified 2-208 8617770
DD TNFR1 Link FADD Co-immunoprecipitation HEK293 Not specified Not specified Not specified 80-208 8617770
DD TNFR1 Link FADD Co-immunoprecipitation L929 10463949
DD TNFR1 Link FADD Co-immunoprecipitation Jurkat Not specified Not specified Not specified Not specified 11002422
DD TNFR1 Link Fas Co-immunoprecipitation C57BL/6J mice macrophage 17692826
DD TNFR1 Link MADD Co-immunoprecipitation COS-7 Not used Not specified Full length 9115275
DD TNFR1 Link MADD Co-immunoprecipitation CCL-131 Not specified Not specified Not specified Not specified 15007167
DD TNFR1 Link RIP Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 8612133
DD TNFR1 Link RIP Co-immunoprecipitation HEK293 Not specified Not specified Not specified Full length 8612133
DD TNFR1 Link RIP Co-immunoprecipitation HEK293 Not specified Not specified Not specified 240-671 8612133
DD TNFR1 Link RIP Co-immunoprecipitation HEK293 Not specified Not specified Not specified 559-671 8612133
DD TNFR1 Link RIP Co-immunoprecipitation HEK293 Not specified Not specified Not specified 37-205 9082980
DD TNFR1 Link RIP Co-immunoprecipitation HEK293 Not specified 37-205 Not specified Not specified 9082980
DD TNFR1 Link RIP Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 9714541
DD TNFR1 Link RIP Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 10358032
DD TNFR1 Link RIP Co-immunoprecipitation HEK293 Not used Human Not specified 10755617
DD TNFR1 Link RIP Co-immunoprecipitation Fibroblast 10795740
DD TNFR1 Link RIP Co-immunoprecipitation Jurkat Not specified Not specified Human Not specified 11002422
DD TNFR1 Link RIP Co-immunoprecipitation U937 11734559
DD TNFR1 Link RIP Co-immunoprecipitation Jurkat 12167698
DD TNFR1 Link RIP Co-immunoprecipitation Jurkat 16611992
DD TNFR1 Link RIP Co-immunoprecipitation Mice peritoneal macrophage lysate 18641653
DD TNFR1 Link RIP Co-immunoprecipitation HT1080 18939944
DD TNFR1 Link RIP Co-immunoprecipitation Jurkat 19524513
DD TNFR1 Link RIP Co-immunoprecipitation H2009 20547836
DD TNFR1 Link TRADD Rat primary hepatocyte 21741934
DD TNFR1 Link TRADD Co-immunoprecipitation HEK293 Human Not specified Human Not specified 7758105
DD TNFR1 Link TRADD Co-immunoprecipitation U937 8565075
DD TNFR1 Link TRADD Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 8612133
DD TNFR1 Link TRADD Co-immunoprecipitation HEK293 Not specified Not specified Human 112-312 8621670
DD TNFR1 Link TRADD Co-immunoprecipitation U937 8943045
DD TNFR1 Link TRADD Co-immunoprecipitation HEK293 Not specified 37-205 Not specified Not specified 9082980
DD TNFR1 Link TRADD Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 9915703
DD TNFR1 Link TRADD Co-immunoprecipitation U937 9916731
DD TNFR1 Link TRADD Co-immunoprecipitation HEK293 Not used Not specified Not specified 10187805
DD TNFR1 Link TRADD Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 10358032
DD TNFR1 Link TRADD Co-immunoprecipitation L929 10463949
DD TNFR1 Link TRADD Co-immunoprecipitation Fibroblast 10795740
DD TNFR1 Link TRADD Co-immunoprecipitation Jurkat Not specified Not specified Not specified Not specified 11002422
DD TNFR1 Link TRADD Co-immunoprecipitation Jurkat 12167698
DD TNFR1 Link TRADD Co-immunoprecipitation Rat brain hippocampus 12786973
DD TNFR1 Link TRADD Co-immunoprecipitation Jurkat 16611992
DD TNFR1 Link TRADD Co-immunoprecipitation L929 16798936
DD TNFR1 Link TRADD Co-immunoprecipitation Human TLE hammacampus lysate 16919273
DD TNFR1 Link TRADD Co-immunoprecipitation HT1080 18939944
DD TNFR1 Link TRADD Co-immunoprecipitation Mouse seizure hippocampal 21760970
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DD TNFR1 Link DR3 Yeast two-hybrid L354A, L356A, D373A (DR3) 8934525
DD TNFR1 Link TRADD GST fusion protein pull-down 196-312 substituted A(TRADD) 8621670
DD TNFR1 Link TRADD GST fusion protein pull-down Q220E, R224E, D246R, F266E, Q267A, A275D (TRADD) R341E, R437A, G403E, E406R (TNFR1) 16006552
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DD TNFR1 Link FADD MTT assay Apoptosis (DISC) 8617770
DD TNFR1 Link RIP Data not shown Apoptosis (DISC) 8612133
DD TNFR1 Link RIP IKK assay NF-kB activation (DISC) 10795740
DD TNFR1 Link RIP Propidium iodide staining Apoptosis (DISC) 11002422
DD TNFR1 Link RIP Propidium iodide staining Apoptosis (DISC) NF-kB activation (DISC) 16611992
DD TNFR1 Link TRADD IKK assay NF-kB activation (DISC) 10795740
DD TNFR1 Link TRADD Propidium iodide staining Apoptosis (DISC) NF-kB activation (DISC) 16611992
(Link: click this icon to show interactions only between the two corresponding DDs)