TRADD (DD)

Full Name:
Tumor necrosis factor receptor type 1-associated DEATH domain protein
Alternative Names:
Hs.89862, MGC11078
Function:
Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.
Death Domain Subfamily & Boundary Region:
  • DD : 179-289 Seq
Species External DB Questionmark
Uniprot DIP STRING KEGG IntAct MINT
Human Seq Q15628external db link DIP-285Nexternal db link Q15628external db link hsa:8717external db link Q15628external db link MINT-99115external db link
Mouse Seq Q3U0V2external db link DIP-38039Nexternal db link Q3U0V2external db link mmu:71609external db link Q3U0V2external db link
Rat Seq D3ZZC5external db link rno:246756external db link
Dog Seq E2REX3external db link
Zebra fish Seq Q9I9N5external db link Q9I9N5external db link
Structure Species Residue Method Expression Purification Column Final condition Final concentration Reference
http://www.pdb.org/pdb/search/structidSearch.do?structureId= External Human 196-301 NMR 17922260
No information
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD TRADD Link DR3 GST fusion protein pull-down Not specified Not specified In vitro translation Human 215-393 E.coli 8875942
DD TRADD Link FADD GST fusion protein pull-down Not specified 243-455 In vitro translation Not specified Full length E.coli 16006552
DD TRADD Link FADD Yeast two-hybrid Not specified Full length Yeast Not specified Full length Yeast 8565075
DD TRADD Link FADD Yeast two-hybrid Not specified 55-312 Yeast Not specified Full length Yeast 8565075
DD TRADD Link FADD Yeast two-hybrid Not specified 195-312 Yeast Not specified Full length Yeast 8565075
DD TRADD Link FADD Yeast two-hybrid Not specified 195-312 Yeast Not specified 92-208 Yeast 8642271
DD TRADD Link FADD Yeast two-hybrid Human Full length Yeast Human 80-208 Yeast 11464214
DD TRADD Link FADD Yeast two-hybrid Not specified Full length Yeast Not specified Full length Yeast 12107169
DD TRADD Link MADD Yeast two-hybrid Not specified 222-289 Yeast Not specified 1281-1356 Yeast 9115275
DD TRADD Link RIP Yeast two-hybrid Not specified 195-312 Yeast Not specified 261-372 Yeast 8642271
DD TRADD Link RIP Yeast two-hybrid Human Full length Yeast Human 580-671 Yeast 10755617
DD TRADD Link TNFR1 GST fusion protein pull-down Human Full length In vitro translation Human Not specified Not specified 7758105
DD TRADD Link TNFR1 GST fusion protein pull-down Human 1-305 In vitro translation Human Not specified Not specified 7758105
DD TRADD Link TNFR1 GST fusion protein pull-down Human 195-312 In vitro translation Human Not specified Not specified 7758105
DD TRADD Link TNFR1 GST fusion protein pull-down Human 196-312 In vitro translation Human 214-426 Not specified 8621670
DD TRADD Link TNFR1 GST fusion protein pull-down Not specified Not specified In vitro translation Not specified Not specified E.coli 8875942
DD TRADD Link TNFR1 GST fusion protein pull-down Not specified Not specified E.coli Not specified 326-426 E.coli 11231290
DD TRADD Link TNFR1 GST fusion protein pull-down Jurkat lysate Not specified 237-455 E.coli 12721308
DD TRADD Link TNFR1 GST fusion protein pull-down Not specified 243-455 In vitro translation Not specified 195-312 E.coli 16006552
DD TRADD Link TNFR1 Yeast two-hybrid Human Full length Yeast Human 214-426 Yeast 7758105
DD TRADD Link TNFR1 Yeast two-hybrid Not specified 195-312 Yeast Not specified 326-426 Yeast 8642271
DD TRADD Link TRADD GST fusion protein pull-down Human 196-312 In vitro translation Human 214-426 Not specified 8621670
DD TRADD Link TRADD Yeast two-hybrid Not specified 195-312 Yeast Not specified 195-312 Yeast 8642271
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD TRADD Link DR3 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 8934525
DD TRADD Link DR3 Co-immunoprecipitation HEK293 Not specified Not specified Human Not specified 9430227
DD TRADD Link DR3 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 9714541
DD TRADD Link DR4 Co-immunoprecipitation HEK293 Human 121-312 Human 106-468 9430227
DD TRADD Link DR4 Co-immunoprecipitation HEK293 Not specified Not specified Not specified 261-465 9430228
DD TRADD Link DR5 Co-immunoprecipitation HEK293 Human 121-312 Human 51-411 9430227
DD TRADD Link DR5 Co-immunoprecipitation HEK293 Not specified Not specified Not specified 261-465 9430228
DD TRADD Link DR5 Co-immunoprecipitation HEK293 Not specified Not specified Not specified 232-439 9430228
DD TRADD Link DR6 Co-immunoprecipitation HEK293 Not specified Not specified Not specified 42-655 9714541
DD TRADD Link FADD Co-Immunofluorescence staining Human TLE hammacampus section 16919273
DD TRADD Link FADD Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 8565075
DD TRADD Link FADD Co-immunoprecipitation HeLa Not specified 195-312 Not specified 19-753 8642271
DD TRADD Link FADD Co-immunoprecipitation HeLa 10521396
DD TRADD Link FADD Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 10521396
DD TRADD Link FADD Co-immunoprecipitation COS-7 Not specified Not specified Not specified Not specified 12796506
DD TRADD Link FADD Co-immunoprecipitation Human TLE hammacampus lysate 16919273
DD TRADD Link FADD Co-immunoprecipitation Mouse seizure hippocampal 21760970
DD TRADD Link Fas Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 11032752
DD TRADD Link Fas Co-immunoprecipitation C57BL/6J mice macrophage 17692826
DD TRADD Link MADD Co-immunoprecipitation CCL-131 Not specified Not specified Not specified Not specified 15007167
DD TRADD Link NGFR Co-Immunofluorescence staining MCF-7 12604596
DD TRADD Link NGFR Co-immunoprecipitation MCF-7 12604596
DD TRADD Link RIP Co-immunoprecipitation HEK293 Not specified Not specified Not specified Full length 8612133
DD TRADD Link RIP Co-immunoprecipitation HEK293 Not specified Not specified Not specified 240-671 8612133
DD TRADD Link RIP Co-immunoprecipitation HEK293 Not specified Not specified Not specified 559-671 8612133
DD TRADD Link RIP Co-immunoprecipitation HeLa Not specified 195-312 Not specified 261-372 8642271
DD TRADD Link RIP Co-immunoprecipitation HeLa 10521396
DD TRADD Link RIP Co-immunoprecipitation HEK293 Not specified Not specified Not used 10521396
DD TRADD Link RIP Co-immunoprecipitation Rat brain hippocampus 12786973
DD TRADD Link TNFR1 Rat primary hepatocyte 21741934
DD TRADD Link TNFR1 Co-immunoprecipitation HEK293 Human Not specified Human Not specified 7758105
DD TRADD Link TNFR1 Co-immunoprecipitation U937 8565075
DD TRADD Link TNFR1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 8612133
DD TRADD Link TNFR1 Co-immunoprecipitation HEK293 Human 112-312 Not specified Not specified 8621670
DD TRADD Link TNFR1 Co-immunoprecipitation U937 8943045
DD TRADD Link TNFR1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified 37-205 9082980
DD TRADD Link TNFR1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 9915703
DD TRADD Link TNFR1 Co-immunoprecipitation U937 9916731
DD TRADD Link TNFR1 Co-immunoprecipitation HEK293 Not specified Not specified Not used 10187805
DD TRADD Link TNFR1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 10358032
DD TRADD Link TNFR1 Co-immunoprecipitation L929 10463949
DD TRADD Link TNFR1 Co-immunoprecipitation Fibroblast 10795740
DD TRADD Link TNFR1 Co-immunoprecipitation Jurkat Not specified Not specified Not specified Not specified 11002422
DD TRADD Link TNFR1 Co-immunoprecipitation Jurkat 12167698
DD TRADD Link TNFR1 Co-immunoprecipitation Rat brain hippocampus 12786973
DD TRADD Link TNFR1 Co-immunoprecipitation Jurkat 16611992
DD TRADD Link TNFR1 Co-immunoprecipitation L929 16798936
DD TRADD Link TNFR1 Co-immunoprecipitation Human TLE hammacampus lysate 16919273
DD TRADD Link TNFR1 Co-immunoprecipitation HT1080 18939944
DD TRADD Link TNFR1 Co-immunoprecipitation Mouse seizure hippocampal 21760970
DD TRADD Link TRADD Co-immunoprecipitation HEK293 Human 112-312 Human 1-312 8621670
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DD TRADD Link FADD GST fusion protein pull-down D246R, E255R, Q267A, R278A (TRADD) R113E, R117E, D123R, D175R (FADD) 16006552
DD TRADD Link TNFR1 GST fusion protein pull-down 196-312 substituted A(TRADD) 8621670
DD TRADD Link TNFR1 GST fusion protein pull-down Q220E, R224E, D246R, F266E, Q267A, A275D (TRADD) R341E, R437A, G403E, E406R (TNFR1) 16006552
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DD TRADD Link DR4 Luciferase assay NF-kB activation 9430227
DD TRADD Link DR5 Luciferase assay NF-kB activation 9430227
DD TRADD Link MADD Annexin V staining, Immunoprecipitation Apoptosis inhibition, TRADD-TNFR1 interaction block 15007167
DD TRADD Link NGFR Co-Immunofluorescence staining NF-kB activation 12604596
DD TRADD Link TNFR1 IKK assay NF-kB activation (DISC) 10795740
DD TRADD Link TNFR1 Propidium iodide staining Apoptosis (DISC) NF-kB activation (DISC) 16611992
(Link: click this icon to show interactions only between the two corresponding DDs)