J Biol Chem. 1999 Apr 9;274(15):10287-92.

mE10, a novel caspase recruitment domain-containing proapoptotic molecule.External

Yan, M., Lee, J., Schilbach, S., Goddard, A., Dixit, V.,
--- - Department of Molecular Oncology, Genentech, Inc., South San Francisco, California 94080, USA.
Apoptotic signaling is mediated by homophilic interactions between conserved domains present in components of the death pathway. The death domain, death effector domain, and caspase recruitment domain (CARD) are examples of such interaction motifs. We have identified a novel mammalian CARD-containing adaptor molecule termed mE10 (mammalian E10). The N-terminal CARD of mE10 exhibits significant homology (47% identity and 64% similarity) to the CARD of a gene from Equine Herpesvirus type 2. The C-terminal region is unique. Overexpression of mE10 in MCF-7 human breast carcinoma cells induces apoptosis. Mutational analysis indicates that CARD-mediated mE10 oligomerization is essential for killing activity. The C terminus of mE10 bound to the zymogen form of caspase-9 and promoted its processing to the active dimeric species. Taken together, these data suggest a model where autoproteolytic activation of pro-caspase-9 is mediated by mE10-induced oligomerization.
PMID: 10187815External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD Bcl-10 Link Bcl-10 GST fusion protein pull-down HEK293 Human Full length Human Full length 10187815
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
CARD Bcl-10 Link Bcl-10 GST fusion protein pull-down L47A, I46A, L41A, I55A (Bcl-10) 10187815
(Link: click this icon to show interactions only between the two corresponding DDs)