J Biol Chem. 1999 May 21;274(21):14560-7.

Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-kappaB.External

Inohara, N., Koseki, T., del Peso, L., Hu, Y., Yee, C., Chen, S., Carrio, R., Merino, J., Liu, D., Ni, J., Nunez, G.,
--- - Department of Pathology and Comprehensive Cancer Center, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
Ced-4 and Apaf-1 belong to a major class of apoptosis regulators that contain caspase-recruitment (CARD) and nucleotide-binding oligomerization domains. Nod1, a protein with an NH2-terminal CARD-linked to a nucleotide-binding domain and a COOH-terminal segment with multiple leucine-rich repeats, was identified. Nod-1 was found to bind to multiple caspases with long prodomains, but specifically activated caspase-9 and promoted caspase-9-induced apoptosis. As reported for Apaf-1, Nod1 required both the CARD and P-loop for function. Unlike Apaf-1, Nod1 induced activation of nuclear factor-kappa-B (NF-kappaB) and bound RICK, a CARD-containing kinase that also induces NF-kappaB activation. Nod1 mutants inhibited NF-kappaB activity induced by RICK, but not that resulting from tumor necrosis factor-alpha stimulation. Thus, Nod1 is a leucine-rich repeat-containing Apaf-1-like molecule that can regulate both apoptosis and NF-kappaB activation pathways.
PMID: 10329646External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD Caspase1 Link NOD1 Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 10329646
CARD Caspase2 Link NOD1 Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 10329646
CARD Caspase4 Link NOD1 Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 10329646
CARD Caspase9 Link NOD1 Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 10329646
CARD NOD1 Link NOD1 Co-immunoprecipitation HEK293 Human Full length Human Full length 10329646
CARD NOD1 Link RIPK2 Co-immunoprecipitation HEK293 Human Full length Not specified 374-540 10329646
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
CARD Caspase9 Link NOD1 Co-immunoprecipitation V41Q(NOD1) 10329646
CARD NOD1 Link RIPK2 Co-immunoprecipitation V41Q(NOD1) 10329646
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
CARD Caspase9 Link NOD1 Caspase cleaved assay, β-galactosidase staining Caspase9 activation, Caspase9 induced Apoptosis 10329646
CARD NOD1 Link RIPK2 Luciferase assay NF-kB activation 10329646
(Link: click this icon to show interactions only between the two corresponding DDs)