J Biol Chem. 1999 Jul 2;274(27):19403-10.

IRAK-M is a novel member of the Pelle/interleukin-1 receptor-associated kinase (IRAK) family.External

Wesche, H., Gao, X., Li, X., Kirschning, C. J., Stark, G. R., Cao, Z.,
--- - Tularik Inc., South San Francisco, California 94080, USA.
The interleukin-1 receptor-associated kinase (IRAK) was first described as a signal transducer for interleukin-1 (IL-1) and has later been implicated in signal transduction of other members of the Toll/IL-1 receptor family. We now report the identification and characterization of a novel IRAK-like molecule. In contrast to the ubiquitously expressed IRAK and IRAK-2, this new IRAK-like molecule is found mainly in cells of monomyeloic origin and is, therefore, designated IRAK-M. Although IRAK-M and IRAK-2 exhibit only a negligible autophosphorylation activity, they can reconstitute the IL-1 response in a 293 mutant cell line lacking IRAK. In addition, we show for the first time that members of the IRAK family are indispensable elements of lipopolysaccharide signal transduction. The discovery of IRAK-M adds another level of complexity to our understanding of signaling by members of the Toll/IL-1 receptor family.
PMID: 10383454External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD IRAK1 Link IRAK1 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 10383454
DD IRAK1 Link IRAK2 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 10383454
DD IRAK1 Link IRAK3 Co-immunoprecipitation HEK293 Not specified Full length Human Full length 10383454
DD IRAK1 Link Myd88 Co-immunoprecipitation HEK293 Not specified Full length Not specified 1-151 10383454
DD IRAK2 Link IRAK2 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 10383454
DD IRAK2 Link IRAK3 Co-immunoprecipitation HEK293 Not specified Full length Human Full length 10383454
DD IRAK2 Link Myd88 Co-immunoprecipitation HEK293 Not specified Full length Not specified 1-151 10383454
DD IRAK3 Link IRAK3 Co-immunoprecipitation HEK293 Human Full length Human Full length 10383454
DD IRAK3 Link Myd88 Co-immunoprecipitation HEK293 Human Full length Not specified 1-151 10383454
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DD IRAK1 Link IRAK2 Kinase assay IRAK2 activation 10383454
DD IRAK1 Link IRAK3 Kinase assay IRAK3 activation 10383454
(Link: click this icon to show interactions only between the two corresponding DDs)