J Neurosci. 1999 Oct 1;19(19):8244-51.
Knock-out of the neural death effector domain protein PEA-15 demonstrates that its expression protects astrocytes from TNFalpha-induced apoptosis.
Kitsberg, D., Formstecher, E., Fauquet, M., Kubes, M., Cordier, J., Canton, B., Pan, G., Rolli, M., Glowinski, J., Chneiweiss, H.,
--- - Harvard Medical School, Boston, Massachusetts 02115, USA.
--- - Harvard Medical School, Boston, Massachusetts 02115, USA.
Apoptosis is a very general phenomenon, but only a few reports concern astrocytes. Indeed, astrocytes express receptors for tumor necrosis factor (TNF) alpha, a cytokine demonstrated on many cells and tissues to mediate apoptosis after recruitment of adaptor proteins containing a death effector domain (DED). PEA-15 is a DED-containing protein prominently expressed in the CNS and particularly abundant in astrocytes. This led us to investigate if PEA-15 expression could be involved in astrocytic protection against deleterious effects of TNF. In vitro assays evidence that PEA-15 may bind to DED-containing protein FADD and caspase-8 known to be apical adaptors of the TNF apoptotic signaling. After generation of PEA-15 null mutant mice, our results demonstrate that PEA-15 expression increases astrocyte survival after exposure to TNF.
PMID: 10493725

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In vitro interaction | |||||||||||||
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DD1 | DD2 | Reference | |||||||||||
Family | DD1 | DD2 | Method | Species | Region | Expression | Species | Region | Expression | ||||
DED | FADD |
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PEA15 | GST fusion protein pull-down | Not specified | Not specified | In vitro translation | Not specified | Not specified | In vitro translation | 10493725 |
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: click this icon to show interactions only between the two corresponding DDs)

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Functional Role | |||||||||||
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Family | DD1 | DD2 | Method | Death-related | Death-unrelated | Reference | |||||
DED | FADD |
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PEA15 | DAPI, Annexin V staining, TUNEL assay | Apoptosis inhibition | 10493725 |
(
: click this icon to show interactions only between the two corresponding DDs)
