J Neurosci. 1999 Oct 1;19(19):8244-51.
Knock-out of the neural death effector domain protein PEA-15 demonstrates that its expression protects astrocytes from TNFalpha-induced apoptosis.
Kitsberg, D., Formstecher, E., Fauquet, M., Kubes, M., Cordier, J., Canton, B., Pan, G., Rolli, M., Glowinski, J., Chneiweiss, H.,
--- - Harvard Medical School, Boston, Massachusetts 02115, USA.
--- - Harvard Medical School, Boston, Massachusetts 02115, USA.
Apoptosis is a very general phenomenon, but only a few reports concern astrocytes. Indeed, astrocytes express receptors for tumor necrosis factor (TNF) alpha, a cytokine demonstrated on many cells and tissues to mediate apoptosis after recruitment of adaptor proteins containing a death effector domain (DED). PEA-15 is a DED-containing protein prominently expressed in the CNS and particularly abundant in astrocytes. This led us to investigate if PEA-15 expression could be involved in astrocytic protection against deleterious effects of TNF. In vitro assays evidence that PEA-15 may bind to DED-containing protein FADD and caspase-8 known to be apical adaptors of the TNF apoptotic signaling. After generation of PEA-15 null mutant mice, our results demonstrate that PEA-15 expression increases astrocyte survival after exposure to TNF.
PMID: 10493725
 In vitro interaction
In vivo interaction
Characterization
Functional role
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| In vitro interaction | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| DD1 | DD2 | Reference | |||||||||||
| Family | DD1 | DD2 | Method | Species | Region | Expression | Species | Region | Expression | ||||
| DED | FADD |
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PEA15 | GST fusion protein pull-down | Not specified | Not specified | In vitro translation | Not specified | Not specified | In vitro translation | 10493725 | ||
(: click this icon to show interactions only between the two corresponding DDs)
: click this icon to show interactions only between the two corresponding DDs)
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In vitro interaction
In vivo interaction
Characterization
Functional role
|
top
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| Functional Role | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Family | DD1 | DD2 | Method | Death-related | Death-unrelated | Reference | |||||
| DED | FADD |
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PEA15 | DAPI, Annexin V staining, TUNEL assay | Apoptosis inhibition | 10493725 | |||||
(: click this icon to show interactions only between the two corresponding DDs)
: click this icon to show interactions only between the two corresponding DDs)
