J Biol Chem. 2001 Feb 16;276(7):4812-8. Epub 2000 Nov 21.

Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-kappaB.External

Ogura, Y., Inohara, N., Benito, A., Chen, F. F., Yamaoka, S., Nunez, G.,
--- - Department of Pathology and Comprehensive Cancer Center, the University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Apaf-1 and Nod1 are members of a protein family, each of which contains a caspase recruitment domain (CARD) linked to a nucleotide-binding domain, which regulate apoptosis and/or NF-kappaB activation. Nod2, a third member of the family, was identified. Nod2 is composed of two N-terminal CARDs, a nucleotide-binding domain, and multiple C-terminal leucine-rich repeats. Although Nod1 and Apaf-1 were broadly expressed in tissues, the expression of Nod2 was highly restricted to monocytes. Nod2 induced nuclear factor kappaB (NF-kappaB) activation, which required IKKgamma and was inhibited by dominant negative mutants of IkappaBalpha, IKKalpha, IKKbeta, and IKKgamma. Nod2 interacted with the serine-threonine kinase RICK via a homophilic CARD-CARD interaction. Furthermore, NF-kappaB activity induced by Nod2 correlated with its ability to interact with RICK and was specifically inhibited by a truncated mutant form of RICK containing its CARD. The identification of Nod2 defines a subfamily of Apaf-1-like proteins that function through RICK to activate a NF-kappaB signaling pathway.
PMID: 11087742External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD NOD2 Link RIPK2 Co-immunoprecipitation HEK293 Human Full length Not specified Full length 11087742
CARD NOD2 Link RIPK2 Co-immunoprecipitation HEK293 Human 1-744 Not specified Full length 11087742
CARD NOD2 Link RIPK2 Co-immunoprecipitation HEK293 Human 1-301 Not specified Full length 11087742
CARD NOD2 Link RIPK2 Co-immunoprecipitation HEK293 Human Full length Not specified 374-540 11087742
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
CARD NOD2 Link RIPK2 Luciferase assay NF-kB activation(Nodosome) 11087742
(Link: click this icon to show interactions only between the two corresponding DDs)